دورية أكاديمية

Western equine encephalitis virus virus-like particles from an insect cell-baculovirus system elicit the strong immune responses in mice.

التفاصيل البيبلوغرافية
العنوان: Western equine encephalitis virus virus-like particles from an insect cell-baculovirus system elicit the strong immune responses in mice.
المؤلفون: Ma J; Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Changchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Changchun, Jilin, China.; College of Life Science and Biotechnology, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang, China., Wang H; Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Changchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Changchun, Jilin, China., Zheng X; Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Changchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Changchun, Jilin, China., Wu H; Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Changchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Changchun, Jilin, China., Yang S; Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Changchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Changchun, Jilin, China., Xia X; Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Changchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Changchun, Jilin, China.
المصدر: Biotechnology journal [Biotechnol J] 2021 Aug; Vol. 16 (8), pp. e2100008. Date of Electronic Publication: 2021 Jul 09.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Wiley-VCH Verlag Country of Publication: Germany NLM ID: 101265833 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1860-7314 (Electronic) Linking ISSN: 18606768 NLM ISO Abbreviation: Biotechnol J Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Weinheim : Wiley-VCH Verlag, c2006-
مواضيع طبية MeSH: Baculoviridae*/genetics , Encephalitis Virus, Western Equine*/genetics, Animals ; Antibodies, Viral ; Horses ; Immunity ; Immunization ; Insecta ; Mice
مستخلص: Western equine encephalitis virus (WEEV) causes lethal encephalitis in humans and equines, and it poses a serious public health threat in many countries. Therefore, the development of an efficient vaccine remains an important challenge for the prevention of WEEV infection. This study presents the first description of WEEV virus-like particles (VLPs) generated from insect cells using recombinant baculoviruses. WEEV VLPs with 206 adjuvant could trigger a strong cellular immune response; increase the levels of IL-2, IL-4 and IFN-γ; and induce a high level of neutralizing antibodies against WEEV in mice. These data showed that the insect cell-baculovirus system is suitable for the production of WEEV VLPs and that these VLPs could elicit the strong immunogenicity in mice. These results suggest a new, nonreplicating, and effective vaccine candidate against WEEV infection.
(© 2021 Wiley-VCH GmbH.)
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معلومات مُعتمدة: 2013BAD12B04 National Science and Technology Support Program of China
فهرسة مساهمة: Keywords: baculoviruses, cytokine; neutralizing antibodies, Sf9 cells, virus-like particles, western equine encephalitis virus
المشرفين على المادة: 0 (Antibodies, Viral)
تواريخ الأحداث: Date Created: 20210627 Date Completed: 20210817 Latest Revision: 20210817
رمز التحديث: 20221213
DOI: 10.1002/biot.202100008
PMID: 34176228
قاعدة البيانات: MEDLINE
الوصف
تدمد:1860-7314
DOI:10.1002/biot.202100008