دورية أكاديمية
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Cdc42 localized in the CatSper signaling complex regulates cAMP-dependent pathways in mouse sperm.

التفاصيل البيبلوغرافية
العنوان: Cdc42 localized in the CatSper signaling complex regulates cAMP-dependent pathways in mouse sperm.
المؤلفون: Luque GM; Instituto de Biología y Medicina Experimental (IBYME-CONICET), Ciudad Autónoma de Buenos Aires, Argentina., Xu X; Department of Electrical and Computer Engineering, Colorado State University, Fort Collins, CO, USA., Romarowski A; Instituto de Biología y Medicina Experimental (IBYME-CONICET), Ciudad Autónoma de Buenos Aires, Argentina.; Department of Veterinary and Animal Science, University of Massachusetts, Amherst, MA, USA., Gervasi MG; Department of Veterinary and Animal Science, University of Massachusetts, Amherst, MA, USA., Orta G; Instituto de Biotecnología, UNAM, Cuernavaca, México., De la Vega-Beltrán JL; Instituto de Biotecnología, UNAM, Cuernavaca, México., Stival C; Instituto de Biología Molecular y Celular de Rosario (CONICET-UNR), Rosario, Santa Fe, Argentina., Gilio N; Instituto de Biología y Medicina Experimental (IBYME-CONICET), Ciudad Autónoma de Buenos Aires, Argentina., Dalotto-Moreno T; Instituto de Biología y Medicina Experimental (IBYME-CONICET), Ciudad Autónoma de Buenos Aires, Argentina., Krapf D; Instituto de Biología Molecular y Celular de Rosario (CONICET-UNR), Rosario, Santa Fe, Argentina., Visconti PE; Department of Veterinary and Animal Science, University of Massachusetts, Amherst, MA, USA., Krapf D; Department of Electrical and Computer Engineering, Colorado State University, Fort Collins, CO, USA., Darszon A; Instituto de Biotecnología, UNAM, Cuernavaca, México., Buffone MG; Instituto de Biología y Medicina Experimental (IBYME-CONICET), Ciudad Autónoma de Buenos Aires, Argentina.
المصدر: FASEB journal : official publication of the Federation of American Societies for Experimental Biology [FASEB J] 2021 Aug; Vol. 35 (8), pp. e21723.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Federation of American Societies for Experimental Biology Country of Publication: United States NLM ID: 8804484 Publication Model: Print Cited Medium: Internet ISSN: 1530-6860 (Electronic) Linking ISSN: 08926638 NLM ISO Abbreviation: FASEB J Subsets: MEDLINE
أسماء مطبوعة: Publication: 2020- : [Bethesda, Md.] : Hoboken, NJ : Federation of American Societies for Experimental Biology ; Wiley
Original Publication: [Bethesda, Md.] : The Federation, [c1987-
مواضيع طبية MeSH: Calcium Channels/*metabolism , Spermatozoa/*metabolism , cdc42 GTP-Binding Protein/*metabolism, Animals ; Calcium/metabolism ; Calcium Channels/deficiency ; Calcium Channels/genetics ; Calcium Signaling ; Cyclic AMP/metabolism ; Female ; Fertilization in Vitro ; Male ; Mice ; Mice, Inbred BALB C ; Mice, Inbred C57BL ; Mice, Knockout ; Models, Biological ; Signal Transduction ; Sperm Capacitation/physiology ; Sperm Motility/physiology ; Sperm Tail/metabolism ; Spermatozoa/drug effects ; Spermatozoa/ultrastructure ; cdc42 GTP-Binding Protein/antagonists & inhibitors
مستخلص: Sperm acquire the ability to fertilize in a process called capacitation and undergo hyperactivation, a change in the motility pattern, which depends on Ca 2+ transport by CatSper channels. CatSper is essential for fertilization and it is subjected to a complex regulation that is not fully understood. Here, we report that similar to CatSper, Cdc42 distribution in the principal piece is confined to four linear domains and this localization is disrupted in CatSper1-null sperm. Cdc42 inhibition impaired CatSper activity and other Ca 2+ -dependent downstream events resulting in a severe compromise of the sperm fertilizing potential. We also demonstrate that Cdc42 is essential for CatSper function by modulating cAMP production by soluble adenylate cyclase (sAC), providing a new regulatory mechanism for the stimulation of CatSper by the cAMP-dependent pathway. These results reveal a broad mechanistic insight into the regulation of Ca 2+ in mammalian sperm, a matter of critical importance in male infertility as well as in contraception.
(© 2021 Federation of American Societies for Experimental Biology.)
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فهرسة مساهمة: Keywords: calcium; capacitation; hyperactivation; sperm; super-resolution
المشرفين على المادة: 0 (Calcium Channels)
0 (Catsper1 protein, mouse)
0 (Cdc42 protein, mouse)
E0399OZS9N (Cyclic AMP)
EC 3.6.5.2 (cdc42 GTP-Binding Protein)
SY7Q814VUP (Calcium)
تواريخ الأحداث: Date Created: 20210705 Date Completed: 20210719 Latest Revision: 20210729
رمز التحديث: 20240829
DOI: 10.1096/fj.202002773RR
PMID: 34224609
قاعدة البيانات: MEDLINE
الوصف
تدمد:1530-6860
DOI:10.1096/fj.202002773RR