دورية أكاديمية
Conformational and Structural Characterization of Carbohydrates and their Interactions Studied by NMR.
| العنوان: | Conformational and Structural Characterization of Carbohydrates and their Interactions Studied by NMR. |
|---|---|
| المؤلفون: | Cañada FJ; Structural and Chemical Biology Department, Centro de Investigaciones Biológicas Margarita Salas, CSIC, 28040 Madrid, Spain | Centro de Investigación Biomédica en Red-Enfermedades Respiratorias (CIBERES), Avenida de Monforte de Lemos 3-5, 28029 Madrid, Spain., Canales Á; Departamento de Química Orgánica I, Facultad Ciencias Químicas, Universidad Complutense de Madrid, Avd. Complutense s/n, C.P. 28040 Madrid, Spain., Valverde P; Structural and Chemical Biology Department, Centro de Investigaciones Biológicas Margarita Salas, CSIC, 28040 Madrid, Spain., de Toro BF; Structural and Chemical Biology Department, Centro de Investigaciones Biológicas Margarita Salas, CSIC, 28040 Madrid, Spain., Martínez-Orts M; Departamento de Química Orgánica I, Facultad Ciencias Químicas, Universidad Complutense de Madrid, Avd. Complutense s/n, C.P. 28040 Madrid, Spain., Phillips PO; Structural and Chemical Biology Department, Centro de Investigaciones Biológicas Margarita Salas, CSIC, 28040 Madrid, Spain., Pereda A; Structural and Chemical Biology Department, Centro de Investigaciones Biológicas Margarita Salas, CSIC, 28040 Madrid, Spain. |
| المصدر: | Current medicinal chemistry [Curr Med Chem] 2022; Vol. 29 (7), pp. 1147-1172. |
| نوع المنشور: | Journal Article; Review |
| اللغة: | English |
| بيانات الدورية: | Publisher: Bentham Science Publishers Country of Publication: United Arab Emirates NLM ID: 9440157 Publication Model: Print Cited Medium: Internet ISSN: 1875-533X (Electronic) Linking ISSN: 09298673 NLM ISO Abbreviation: Curr Med Chem Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Saif Zone, Sharjah, U.A.E. : Bentham Science Publishers Original Publication: Schiphol, The Netherlands : Bentham Science Publishers, |
| مواضيع طبية MeSH: | Carbohydrates*/chemistry , Polysaccharides*/chemistry, Humans ; Magnetic Resonance Spectroscopy/methods ; Models, Molecular ; Molecular Conformation |
| مستخلص: | Carbohydrates, either free or as glycans conjugated with other biomolecules, participate in a plethora of essential biological processes. Their apparent simplicity in terms of chemical functionality hides an extraordinary diversity and structural complexity. Deeply deciphering at the atomic level their structures is essential to understand their biological function and activities, but it is still a challenging task in need of complementary approaches and no generalized procedures are available to address the study of such complex, natural glycans. The versatility of Nuclear Magnetic Resonance spectroscopy (NMR) often makes it the preferred choice to study glycans and carbohydrates in solution media. The most basic NMR parameters, namely chemical shifts, coupling constants, and nuclear Overhauser effects, allow defining short or repetitive chain sequences and characterize their structures and local geometries either in the free state or when interacting with other biomolecules, rendering additional information on the molecular recognition processes. The increased accessibility to carbohydrate molecules extensively or selectively labeled with 13 C is boosting the resolution and detail which analyzed glycan structures can reach. In turn, structural information derived from NMR complemented with molecular modeling and theoretical calculations can also provide dynamic information on the conformational flexibility of carbohydrate structures. Furthermore, using partially oriented media or paramagnetic perturbations, it has been possible to introduce additional longrange observables rendering structural information on longer and branched glycan chains. In this review, we provide examples of these studies and an overview of the recent and most relevant NMR applications in the glycobiology field. (Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.) |
| معلومات مُعتمدة: | PID2019-1052376B-I00, CTQ2016-76263-P, RTI2018-094751-B-C22 Agencia Estatal de Investigación; S2017/BMD-3673 Spanish Regional Government of Madrid |
| فهرسة مساهمة: | Keywords: Carbohydrates; NMR; conformational analysis; glycans; molecular recognition; structure |
| المشرفين على المادة: | 0 (Carbohydrates) 0 (Polysaccharides) |
| تواريخ الأحداث: | Date Created: 20210706 Date Completed: 20220302 Latest Revision: 20220302 |
| رمز التحديث: | 20240628 |
| DOI: | 10.2174/0929867328666210705154046 |
| PMID: | 34225601 |
| قاعدة البيانات: | MEDLINE |
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