دورية أكاديمية
أعرض في EDS

Single-nucleus RNA-seq2 reveals functional crosstalk between liver zonation and ploidy.

التفاصيل البيبلوغرافية
العنوان: Single-nucleus RNA-seq2 reveals functional crosstalk between liver zonation and ploidy.
المؤلفون: Richter ML; Helmholtz Pioneer Campus (HPC), Helmholtz Zentrum München, Neuherberg, Germany., Deligiannis IK; Helmholtz Pioneer Campus (HPC), Helmholtz Zentrum München, Neuherberg, Germany., Yin K; Helmholtz Pioneer Campus (HPC), Helmholtz Zentrum München, Neuherberg, Germany.; University of Cambridge, Cancer Research UK Cambridge Institute, Robinson Way, Cambridge, United Kingdom., Danese A; Institute of Computational Biology, Helmholtz Zentrum München, Neuherberg, Germany., Lleshi E; University of Cambridge, Cancer Research UK Cambridge Institute, Robinson Way, Cambridge, United Kingdom., Coupland P; University of Cambridge, Cancer Research UK Cambridge Institute, Robinson Way, Cambridge, United Kingdom., Vallejos CA; MRC Human Genetics Unit, Institute of Genetics & Molecular Medicine, University of Edinburgh, Western General Hospital, Edinburgh, United Kingdom., Matchett KP; Centre for Inflammation Research, The Queen's Medical Research Institute, University of Edinburgh, Little France Crescent, Edinburgh, United Kingdom., Henderson NC; MRC Human Genetics Unit, Institute of Genetics & Molecular Medicine, University of Edinburgh, Western General Hospital, Edinburgh, United Kingdom.; Centre for Inflammation Research, The Queen's Medical Research Institute, University of Edinburgh, Little France Crescent, Edinburgh, United Kingdom., Colome-Tatche M; Institute of Computational Biology, Helmholtz Zentrum München, Neuherberg, Germany. maria.colome@helmholtz-muenchen.de.; TUM School of Life Sciences Weihenstephan, Technical University of Munich, Freising, Germany. maria.colome@helmholtz-muenchen.de.; Biomedical Center (BMC), Physiological Chemistry, Faculty of Medicine, LMU Munich, Munich, Germany. maria.colome@helmholtz-muenchen.de., Martinez-Jimenez CP; Helmholtz Pioneer Campus (HPC), Helmholtz Zentrum München, Neuherberg, Germany. celia.martinez@helmholtz-muenchen.de.; TUM School of Medicine, Technical University of Munich, Munich, Germany. celia.martinez@helmholtz-muenchen.de.
المصدر: Nature communications [Nat Commun] 2021 Jul 12; Vol. 12 (1), pp. 4264. Date of Electronic Publication: 2021 Jul 12.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Nature Pub. Group Country of Publication: England NLM ID: 101528555 Publication Model: Electronic Cited Medium: Internet ISSN: 2041-1723 (Electronic) Linking ISSN: 20411723 NLM ISO Abbreviation: Nat Commun Subsets: MEDLINE
أسماء مطبوعة: Original Publication: [London] : Nature Pub. Group
مواضيع طبية MeSH: Ploidies* , Sequence Analysis, RNA* , Single-Cell Analysis*, Liver/*metabolism, Animals ; Biomarkers/metabolism ; Cell Nucleus/metabolism ; Chronic Disease ; Frozen Sections ; Gene Expression Profiling ; Gene Expression Regulation ; Gene Ontology ; Hepatocytes/metabolism ; Liver/pathology ; Liver Diseases/pathology ; Mice, Inbred C57BL ; Regeneration ; Stem Cells/metabolism ; Transcription Factors/metabolism ; Transcription, Genetic ; Mice
مستخلص: Single-cell RNA-seq reveals the role of pathogenic cell populations in development and progression of chronic diseases. In order to expand our knowledge on cellular heterogeneity, we have developed a single-nucleus RNA-seq2 method tailored for the comprehensive analysis of the nuclear transcriptome from frozen tissues, allowing the dissection of all cell types present in the liver, regardless of cell size or cellular fragility. We use this approach to characterize the transcriptional profile of individual hepatocytes with different levels of ploidy, and have discovered that ploidy states are associated with different metabolic potential, and gene expression in tetraploid mononucleated hepatocytes is conditioned by their position within the hepatic lobule. Our work reveals a remarkable crosstalk between gene dosage and spatial distribution of hepatocytes.
(© 2021. The Author(s).)
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معلومات مُعتمدة: United Kingdom WT_ Wellcome Trust; 219542/Z/19/Z United Kingdom WT_ Wellcome Trust; United Kingdom CRUK_ Cancer Research UK; WT098051 United Kingdom WT_ Wellcome Trust
المشرفين على المادة: 0 (Biomarkers)
0 (Transcription Factors)
تواريخ الأحداث: Date Created: 20210713 Date Completed: 20210721 Latest Revision: 20240226
رمز التحديث: 20240226
مُعرف محوري في PubMed: PMC8275628
DOI: 10.1038/s41467-021-24543-5
PMID: 34253736
قاعدة البيانات: MEDLINE
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