دورية أكاديمية
In vivo antimalarial activity of a probiotic bacterium Lactobacillus sakei isolated from traditionally fermented milk in BALB/c mice infected with Plasmodium berghei ANKA.
| العنوان: | In vivo antimalarial activity of a probiotic bacterium Lactobacillus sakei isolated from traditionally fermented milk in BALB/c mice infected with Plasmodium berghei ANKA. |
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| المؤلفون: | Toukam LL; Department of Microbiology and Parasitology, Faculty of Science, University of Buea, Buea, Cameroon., Tatsinkou Fossi B; Department of Microbiology and Parasitology, Faculty of Science, University of Buea, Buea, Cameroon. Electronic address: tatsinkou.fossi@ubuea.cm., Taiwe GS; Department of Zoology and Animal Physiology, Faculty of Science, University of Buea, Cameroon., Bila RB; Department of Zoology and Animal Physiology, Faculty of Science, University of Buea, Cameroon., Feugaing Sofeu DD; Department of Biochemistry and Molecular Biology, Faculty of Science, University of Buea, Cameroon., Ivo EP; Research Foundation for Tropical Diseases and Environment (REFOTDE) , Cameroon., Achidi EA; Department of Biochemistry and Molecular Biology, Faculty of Science, University of Buea, Cameroon. |
| المصدر: | Journal of ethnopharmacology [J Ethnopharmacol] 2021 Nov 15; Vol. 280, pp. 114448. Date of Electronic Publication: 2021 Jul 22. |
| نوع المنشور: | Comparative Study; Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Sequoia Country of Publication: Ireland NLM ID: 7903310 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1872-7573 (Electronic) Linking ISSN: 03788741 NLM ISO Abbreviation: J Ethnopharmacol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Limerick : Elsevier Sequoia Original Publication: Lausanne, Elsevier Sequoia. |
| مواضيع طبية MeSH: | Latilactobacillus sakei*, Antimalarials/*pharmacology , Malaria/*therapy , Plasmodium berghei/*drug effects , Probiotics/*pharmacology, Animals ; Antimalarials/administration & dosage ; Cameroon ; Chloroquine/pharmacology ; Disease Models, Animal ; Drug Combinations ; Fermented Foods ; Malaria/parasitology ; Mice ; Mice, Inbred BALB C ; Milk/microbiology ; Parasitemia/parasitology ; Parasitemia/therapy ; Probiotics/administration & dosage ; Pyrimethamine/pharmacology ; Sulfadoxine/pharmacology |
| مستخلص: | Ethnopharmacological Relevance: Milk production, processing and consumption are integral part of traditional practices in Fulani tribe of Cameroon. It has been observed that Fulani are resistant to malaria. Dairy products traditionally processed by Fulani are intensively used in the ritual treatment of malarial, inflammations and behavioural disorders. Many studies have demonstrated that fermented milk is a rich source of probiotic bacteria. However, the antimalarial activity of probiotics isolated from this natural source has not been experimentally tested. Aim of the Study: Hence, this study was therefore aimed at evaluating the antimalarial activity of a probiotic bacterium Lactobacillus sakei isolated from traditionally fermented milk in mice infected with chloroquine sensitive Plasmodium berghei ANKA. Materials and Methods: The probiotic bacterium was isolated from the Cameroonian Mborro Fulani's traditionally fermented milk and identified using the 16S r RNA gene sequencing. The schizontocidal activity of Lactobacillus sakei on established malaria infection was evaluated. Eighty-four healthy young adult Balb/c mice infected with Plasmodium berghei parasite were randomly divided into two sets of seven group of six mice each, and were given three different doses of Lactobacillus sakei, chloroquine and sulfadoxine/pyrimethamine for seven and fourteen days respectively. The level of parasitaemia, body temperature, survival time and haematological parameters were evaluated. Results: The parasite growth inhibition was observed to increase with increasing dose of probiotic bacterium with maximum suppression being 100 % at dose 3 on day 20. Also, the probiotic bacterium significantly prevented body weight loss and was associated with body temperature reduction and prevented (p<0.05) a decrease in haematological parameters compared to that untreated malaria infected mice. Conclusion: The results obtained suggest that Lactobacillus sakei is a probiotic bacterium with antimalarial activity in mice infected with chloroquine sensitive Plasmodium berghei. (Copyright © 2021 Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Fermented milk; Fulani; Haematological parameters; Malaria; Parasitaemia; Probiotics |
| المشرفين على المادة: | 0 (Antimalarials) 0 (Drug Combinations) 37338-39-9 (fanasil, pyrimethamine drug combination) 88463U4SM5 (Sulfadoxine) 886U3H6UFF (Chloroquine) Z3614QOX8W (Pyrimethamine) |
| تواريخ الأحداث: | Date Created: 20210725 Date Completed: 20220117 Latest Revision: 20221207 |
| رمز التحديث: | 20240829 |
| DOI: | 10.1016/j.jep.2021.114448 |
| PMID: | 34303805 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1872-7573 |
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| DOI: | 10.1016/j.jep.2021.114448 |