دورية أكاديمية
أعرض في EDS

Single-cell RNA sequencing reveals ex vivo signatures of SARS-CoV-2-reactive T cells through 'reverse phenotyping'.

التفاصيل البيبلوغرافية
العنوان: Single-cell RNA sequencing reveals ex vivo signatures of SARS-CoV-2-reactive T cells through 'reverse phenotyping'.
المؤلفون: Fischer DS; Institute of Computational Biology, Helmholtz Zentrum München, Neuherberg, München, Germany.; TUM School of Life Sciences Weihenstephan, Technical University of Munich, Freising, Germany., Ansari M; Institute of Computational Biology, Helmholtz Zentrum München, Neuherberg, München, Germany.; Institute of Lung Biology and Disease and Comprehensive Pneumology Center with the CPC-M bioArchive, Helmholtz Zentrum Muenchen, Member of the German Center for Lung Research (DZL), Munich, Germany., Wagner KI; Institute for Medical Microbiology, Immunology and Hygiene, Technische Universität München (TUM), Munich, Germany., Jarosch S; Institute for Medical Microbiology, Immunology and Hygiene, Technische Universität München (TUM), Munich, Germany., Huang Y; Institute of Virology, Technische Universität München (TUM), Munich, Germany., Mayr CH; Institute of Lung Biology and Disease and Comprehensive Pneumology Center with the CPC-M bioArchive, Helmholtz Zentrum Muenchen, Member of the German Center for Lung Research (DZL), Munich, Germany., Strunz M; Institute of Lung Biology and Disease and Comprehensive Pneumology Center with the CPC-M bioArchive, Helmholtz Zentrum Muenchen, Member of the German Center for Lung Research (DZL), Munich, Germany., Lang NJ; Institute of Lung Biology and Disease and Comprehensive Pneumology Center with the CPC-M bioArchive, Helmholtz Zentrum Muenchen, Member of the German Center for Lung Research (DZL), Munich, Germany., D'Ippolito E; Institute for Medical Microbiology, Immunology and Hygiene, Technische Universität München (TUM), Munich, Germany., Hammel M; Institute for Medical Microbiology, Immunology and Hygiene, Technische Universität München (TUM), Munich, Germany., Mateyka L; Institute for Medical Microbiology, Immunology and Hygiene, Technische Universität München (TUM), Munich, Germany., Weber S; Institute for Medical Microbiology, Immunology and Hygiene, Technische Universität München (TUM), Munich, Germany., Wolff LS; Institute of Virology, Technische Universität München (TUM), Munich, Germany., Witter K; Laboratory of Immunogenetics and Molecular Diagnostics, Department of Transfusion Medicine, Cell Therapeutic Agents and Hemostaseology, LMU Munich, Munich, Germany.; Department of Medicine V, University Hospital, LMU Munich, Comprehensive Pneumology Center Munich (CPC-M), Member of the German Center for lung research (DZL), Munich, Germany., Fernandez IE; Department of Medicine V, University Hospital, LMU Munich, Comprehensive Pneumology Center Munich (CPC-M), Member of the German Center for lung research (DZL), Munich, Germany., Leuschner G; Department of Medicine V, University Hospital, LMU Munich, Comprehensive Pneumology Center Munich (CPC-M), Member of the German Center for lung research (DZL), Munich, Germany., Milger K; Department of Medicine V, University Hospital, LMU Munich, Comprehensive Pneumology Center Munich (CPC-M), Member of the German Center for lung research (DZL), Munich, Germany., Frankenberger M; Institute of Lung Biology and Disease and Comprehensive Pneumology Center with the CPC-M bioArchive, Helmholtz Zentrum Muenchen, Member of the German Center for Lung Research (DZL), Munich, Germany.; Center for Thoracic Surgery Munich, Ludwig-Maximilians-University of Munich (LMU) and Asklepios Lung Clinic Munich-Gauting, Munich and Gauting, Munich, Germany., Nowak L; Center for Thoracic Surgery Munich, Ludwig-Maximilians-University of Munich (LMU) and Asklepios Lung Clinic Munich-Gauting, Munich and Gauting, Munich, Germany., Heinig-Menhard K; Center for Thoracic Surgery Munich, Ludwig-Maximilians-University of Munich (LMU) and Asklepios Lung Clinic Munich-Gauting, Munich and Gauting, Munich, Germany., Koch I; Institute of Lung Biology and Disease and Comprehensive Pneumology Center with the CPC-M bioArchive, Helmholtz Zentrum Muenchen, Member of the German Center for Lung Research (DZL), Munich, Germany.; Asklepios Biobank for pulmonary diseases, Gauting, Germany.; Member of the German Center for Lung Research (DZL), Center for Comprehensive Developmental Care (CDeCLMU), Department of Neonatology, Perinatal Center, Munich, Germany., Stoleriu MG; Institute of Lung Biology and Disease and Comprehensive Pneumology Center with the CPC-M bioArchive, Helmholtz Zentrum Muenchen, Member of the German Center for Lung Research (DZL), Munich, Germany.; Asklepios Biobank for pulmonary diseases, Gauting, Germany.; Member of the German Center for Lung Research (DZL), Center for Comprehensive Developmental Care (CDeCLMU), Department of Neonatology, Perinatal Center, Munich, Germany., Hilgendorff A; Institute of Lung Biology and Disease and Comprehensive Pneumology Center with the CPC-M bioArchive, Helmholtz Zentrum Muenchen, Member of the German Center for Lung Research (DZL), Munich, Germany.; German Center for Infection Research (DZIF), partner site Munich, Munich, Germany., Behr J; Department of Medicine V, University Hospital, LMU Munich, Comprehensive Pneumology Center Munich (CPC-M), Member of the German Center for lung research (DZL), Munich, Germany.; Center for Thoracic Surgery Munich, Ludwig-Maximilians-University of Munich (LMU) and Asklepios Lung Clinic Munich-Gauting, Munich and Gauting, Munich, Germany., Pichlmair A; Institute of Virology, Technische Universität München (TUM), Munich, Germany.; Department of Mathematics, Technical University of Munich, Garching, Germany., Schubert B; Institute of Computational Biology, Helmholtz Zentrum München, Neuherberg, München, Germany.; Focus Group 'Clinical Cell Processing and Purification', Institute for Advanced Study, TUM, Munich, Germany., Theis FJ; Institute of Computational Biology, Helmholtz Zentrum München, Neuherberg, München, Germany.; Focus Group 'Clinical Cell Processing and Purification', Institute for Advanced Study, TUM, Munich, Germany., Busch DH; Institute for Medical Microbiology, Immunology and Hygiene, Technische Universität München (TUM), Munich, Germany.; Department of Mathematics, Technical University of Munich, Garching, Germany.; Grosshadern, Hospital of the Ludwig-Maximilians University (LMU), Munich, Germany., Schiller HB; Institute of Lung Biology and Disease and Comprehensive Pneumology Center with the CPC-M bioArchive, Helmholtz Zentrum Muenchen, Member of the German Center for Lung Research (DZL), Munich, Germany. herbert.schiller@helmholtz-muenchen.de.; Institute of Lung Biology and Disease, Comprehensive Pneumology Center, Helmholtz Zentrum München, Neuherberg, München, Germany. herbert.schiller@helmholtz-muenchen.de., Schober K; Institute for Medical Microbiology, Immunology and Hygiene, Technische Universität München (TUM), Munich, Germany. kilian.schober@uk-erlangen.de.; Microbiological Institute-Institute of Clinical Microbiology, Immunology and Hygiene, University Hospital of Erlangen, Erlangen, Germany. kilian.schober@uk-erlangen.de.
المصدر: Nature communications [Nat Commun] 2021 Jul 26; Vol. 12 (1), pp. 4515. Date of Electronic Publication: 2021 Jul 26.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Nature Pub. Group Country of Publication: England NLM ID: 101528555 Publication Model: Electronic Cited Medium: Internet ISSN: 2041-1723 (Electronic) Linking ISSN: 20411723 NLM ISO Abbreviation: Nat Commun Subsets: MEDLINE
أسماء مطبوعة: Original Publication: [London] : Nature Pub. Group
مواضيع طبية MeSH: COVID-19/*immunology , Gene Expression Profiling/*methods , Sequence Analysis, RNA/*methods , Single-Cell Analysis/*methods , T-Lymphocytes/*metabolism, Aged ; Aged, 80 and over ; CD4-Positive T-Lymphocytes/metabolism ; CD4-Positive T-Lymphocytes/virology ; COVID-19/epidemiology ; COVID-19/virology ; Cells, Cultured ; Cohort Studies ; Female ; Humans ; Male ; Middle Aged ; Pandemics ; Receptors, Antigen, T-Cell/genetics ; Receptors, Antigen, T-Cell/immunology ; Receptors, Antigen, T-Cell/metabolism ; SARS-CoV-2/physiology ; T-Lymphocytes/virology
مستخلص: The in vivo phenotypic profile of T cells reactive to severe acute respiratory syndrome (SARS)-CoV-2 antigens remains poorly understood. Conventional methods to detect antigen-reactive T cells require in vitro antigenic re-stimulation or highly individualized peptide-human leukocyte antigen (pHLA) multimers. Here, we use single-cell RNA sequencing to identify and profile SARS-CoV-2-reactive T cells from Coronavirus Disease 2019 (COVID-19) patients. To do so, we induce transcriptional shifts by antigenic stimulation in vitro and take advantage of natural T cell receptor (TCR) sequences of clonally expanded T cells as barcodes for 'reverse phenotyping'. This allows identification of SARS-CoV-2-reactive TCRs and reveals phenotypic effects introduced by antigen-specific stimulation. We characterize transcriptional signatures of currently and previously activated SARS-CoV-2-reactive T cells, and show correspondence with phenotypes of T cells from the respiratory tract of patients with severe disease in the presence or absence of virus in independent cohorts. Reverse phenotyping is a powerful tool to provide an integrated insight into cellular states of SARS-CoV-2-reactive T cells across tissues and activation states.
(© 2021. The Author(s).)
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سلسلة جزيئية: figshare 10.6084/m9.figshare.12436517
المشرفين على المادة: 0 (Receptors, Antigen, T-Cell)
تواريخ الأحداث: Date Created: 20210727 Date Completed: 20210809 Latest Revision: 20240813
رمز التحديث: 20240813
مُعرف محوري في PubMed: PMC8313584
DOI: 10.1038/s41467-021-24730-4
PMID: 34312385
قاعدة البيانات: MEDLINE
الوصف
تدمد:2041-1723
DOI:10.1038/s41467-021-24730-4