دورية أكاديمية
A Single-Cell Atlas of Lymphocyte Adaptive Immune Repertoires and Transcriptomes Reveals Age-Related Differences in Convalescent COVID-19 Patients.
| العنوان: | A Single-Cell Atlas of Lymphocyte Adaptive Immune Repertoires and Transcriptomes Reveals Age-Related Differences in Convalescent COVID-19 Patients. |
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| المؤلفون: | Bieberich F; Department of Biosystems Science and Engineering, ETH Zurich, Basel, Switzerland., Vazquez-Lombardi R; Department of Biosystems Science and Engineering, ETH Zurich, Basel, Switzerland., Yermanos A; Department of Biosystems Science and Engineering, ETH Zurich, Basel, Switzerland.; Institute of Microbiology and Immunology, Department of Biology, ETH Zurich, Zurich, Switzerland.; Department of Pathology and Immunology, University of Geneva, Geneva, Switzerland.; Botnar Research Centre for Child Health, Basel, Switzerland., Ehling RA; Department of Biosystems Science and Engineering, ETH Zurich, Basel, Switzerland., Mason DM; Department of Biosystems Science and Engineering, ETH Zurich, Basel, Switzerland.; deepCDR Biologics AG, Basel, Switzerland., Wagner B; Department of Biosystems Science and Engineering, ETH Zurich, Basel, Switzerland., Kapetanovic E; Department of Biosystems Science and Engineering, ETH Zurich, Basel, Switzerland., Di Roberto RB; Department of Biosystems Science and Engineering, ETH Zurich, Basel, Switzerland., Weber CR; Department of Biosystems Science and Engineering, ETH Zurich, Basel, Switzerland.; deepCDR Biologics AG, Basel, Switzerland., Savic M; Department of Biomedical Engineering, University of Basel, Allschwil, Switzerland.; Department of Surgery, Oral and Cranio-Maxillofacial Surgery, University Hospital Basel, Basel, Switzerland.; Department of Health, Economics and Health Directorate, Canton Basel-Landschaft, Switzerland., Rudolf F; Department of Biosystems Science and Engineering, ETH Zurich, Basel, Switzerland., Reddy ST; Department of Biosystems Science and Engineering, ETH Zurich, Basel, Switzerland.; Botnar Research Centre for Child Health, Basel, Switzerland. |
| المصدر: | Frontiers in immunology [Front Immunol] 2021 Jul 12; Vol. 12, pp. 701085. Date of Electronic Publication: 2021 Jul 12 (Print Publication: 2021). |
| نوع المنشور: | Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Frontiers Research Foundation] Country of Publication: Switzerland NLM ID: 101560960 Publication Model: eCollection Cited Medium: Internet ISSN: 1664-3224 (Electronic) Linking ISSN: 16643224 NLM ISO Abbreviation: Front Immunol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: [Lausanne : Frontiers Research Foundation] |
| مواضيع طبية MeSH: | Aging/*immunology , B-Lymphocytes/*immunology , CD4-Positive T-Lymphocytes/*immunology , CD8-Positive T-Lymphocytes/*immunology , COVID-19/*immunology , SARS-CoV-2/*physiology, Adaptive Immunity ; Adult ; Aged ; Cells, Cultured ; Convalescence ; Female ; Humans ; Male ; Middle Aged ; Receptors, Antigen, B-Cell/genetics ; Receptors, Antigen, T-Cell/genetics ; Single-Cell Analysis ; Transcriptome ; Young Adult |
| مستخلص: | COVID-19 disease outcome is highly dependent on adaptive immunity from T and B lymphocytes, which play a critical role in the control, clearance and long-term protection against SARS-CoV-2. To date, there is limited knowledge on the composition of the T and B cell immune receptor repertoires [T cell receptors (TCRs) and B cell receptors (BCRs)] and transcriptomes in convalescent COVID-19 patients of different age groups. Here, we utilize single-cell sequencing (scSeq) of lymphocyte immune repertoires and transcriptomes to quantitatively profile the adaptive immune response in COVID-19 patients of varying age. We discovered highly expanded T and B cells in multiple patients, with the most expanded clonotypes coming from the effector CD8 + T cell population. Highly expanded CD8 + and CD4 + T cell clones show elevated markers of cytotoxicity (CD8: PRF1, GZMH, GNLY; CD4: GZMA), whereas clonally expanded B cells show markers of transition into the plasma cell state and activation across patients. By comparing young and old convalescent COVID-19 patients (mean ages = 31 and 66.8 years, respectively), we found that clonally expanded B cells in young patients were predominantly of the IgA isotype and their BCRs had incurred higher levels of somatic hypermutation than elderly patients. In conclusion, our scSeq analysis defines the adaptive immune repertoire and transcriptome in convalescent COVID-19 patients and shows important age-related differences implicated in immunity against SARS-CoV-2. Competing Interests: Author DM and CW were employed by company deepCDR Biologics AG. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2021 Bieberich, Vazquez-Lombardi, Yermanos, Ehling, Mason, Wagner, Kapetanovic, Di Roberto, Weber, Savic, Rudolf and Reddy.) |
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| فهرسة مساهمة: | Keywords: B cell; COVID-19; SARS-CoV-2; T cell; VDJ repertoire; antibody; single-cell |
| المشرفين على المادة: | 0 (Receptors, Antigen, B-Cell) 0 (Receptors, Antigen, T-Cell) |
| تواريخ الأحداث: | Date Created: 20210729 Date Completed: 20210817 Latest Revision: 20210817 |
| رمز التحديث: | 20231215 |
| مُعرف محوري في PubMed: | PMC8312723 |
| DOI: | 10.3389/fimmu.2021.701085 |
| PMID: | 34322127 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1664-3224 |
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| DOI: | 10.3389/fimmu.2021.701085 |