دورية أكاديمية
[Applying Chitosan-Modified Nanoemulsion in Nasal Vaccine Delivery].
| العنوان: | [Applying Chitosan-Modified Nanoemulsion in Nasal Vaccine Delivery]. |
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| المؤلفون: | Hao XY; Key Laboratory of Drug-Targeting and Drug Delivery System of the Ministry of Education, West China School of Pharmacy, Sichuan University, Chengdu 610041, China., Zhang YD; Key Laboratory of Drug-Targeting and Drug Delivery System of the Ministry of Education, West China School of Pharmacy, Sichuan University, Chengdu 610041, China., Hou YY; Key Laboratory of Drug-Targeting and Drug Delivery System of the Ministry of Education, West China School of Pharmacy, Sichuan University, Chengdu 610041, China., Sun X; Key Laboratory of Drug-Targeting and Drug Delivery System of the Ministry of Education, West China School of Pharmacy, Sichuan University, Chengdu 610041, China. |
| المصدر: | Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition [Sichuan Da Xue Xue Bao Yi Xue Ban] 2021 Jul; Vol. 52 (4), pp. 592-597. |
| نوع المنشور: | Journal Article |
| اللغة: | Chinese |
| بيانات الدورية: | Publisher: Sichuan da xue xue bao (yi xue ban) bian ji bu Country of Publication: China NLM ID: 101162609 Publication Model: Print Cited Medium: Print ISSN: 1672-173X (Print) Linking ISSN: 1672173X NLM ISO Abbreviation: Sichuan Da Xue Xue Bao Yi Xue Ban Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Chengdu Shi : Sichuan da xue xue bao (yi xue ban) bian ji bu, 2003- |
| مواضيع طبية MeSH: | Chitosan* , Nanoparticles* , Vaccines*, Administration, Intranasal ; Animals ; Dogs ; Drug Delivery Systems ; Mice |
| مستخلص: | Objective: To prepare a chitosan-modified cationic nanoemulsion that could be used to prolong the residence time of nasal vaccines in the nasal cavity and improve the cellular uptake efficiency so as to enhance the immune efficacy of nasal vaccines. Methods: A nanoemulsion-based vaccine coated with chitosan was prepared, and the particle size, potential, antigen encapsulation efficiency, stability as well as cytotoxicity were examined. The uptake efficiency of vaccine on different cells and the residence time of vaccine in the nasal cavity were measured. Finally, nasal vaccine was administered on mice and the antibody levels in the serum and in the nasal lavage fluids of the immunized mice were examined. Results: The nanoemulsion-based vaccine had an average particle size of (167.2±0.75) nm, a polydispersity index (PDI) of 0.21±0.01, and an average potential of (13.7±0.85) mV. The encapsulation efficiency of antigen was 92.7%. The nanoemulsion-based vaccine had good stability and did not show obvious cytotoxicity in Madin-Darby canine kidney (MDCK) epithelial cells. The vaccine demonstrated relatively high cellular uptake of antigens on DC2.4 and MDCK cells at (49.7±3.45)% and (59.7±2.19)%, respectively. Besides, the cationic nanoemulsion also significantly increased the residence time of the antigen, and a considerable amount of nanoemulsion-based vaccine was found remaining in the nasal cavity 60 minutes after administration. Compared with free antigen and the nanoemulsion without chitosan modification, the chitosan-modified nanoemulsion vaccine induced higher systemic and mucosal antibody levels in mice after nasal immunization ( P <0.01). Conclusion: The chitosan-modified nanoemulsion vaccine prepared in the study can enhance the immune efficacy of nasal vaccines, showing great potential to be used as a delivery carrier for nasal vaccines. (Copyright© by Editorial Board of Journal of Sichuan University (Medical Sciences).) |
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| فهرسة مساهمة: | Keywords: Immune responses; Nanoemulsion; Nasal administration |
| المشرفين على المادة: | 0 (Vaccines) 9012-76-4 (Chitosan) |
| تواريخ الأحداث: | Date Created: 20210729 Date Completed: 20210730 Latest Revision: 20230829 |
| رمز التحديث: | 20231215 |
| مُعرف محوري في PubMed: | PMC10409387 |
| DOI: | 10.12182/20210760104 |
| PMID: | 34323036 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1672-173X |
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| DOI: | 10.12182/20210760104 |