Smart Design of a pH-Responsive System Based on pHLIP-Modified Magnetite Nanoparticles for Tumor MRI.

التفاصيل البيبلوغرافية
العنوان:	Smart Design of a pH-Responsive System Based on pHLIP-Modified Magnetite Nanoparticles for Tumor MRI.
المؤلفون:	Demin AM; Postovsky Institute of Organic Synthesis, Russian Academy of Sciences (Ural Branch), 620108 Yekaterinburg, Russia., Pershina AG; Siberian State Medical University, 634050 Tomsk, Russia.; Research School of Chemical and Biomedical Engineering, National Research Tomsk Polytechnic University, 634050 Tomsk, Russia., Minin AS; Mikheev Institute of Metal Physics, Russian Academy of Sciences (Ural Branch), 620990 Yekaterinburg, Russia., Brikunova OY; Research School of Chemical and Biomedical Engineering, National Research Tomsk Polytechnic University, 634050 Tomsk, Russia., Murzakaev AM; Institute of Electrophysics, Russian Academy of Sciences (Ural Branch), 620016 Yekaterinburg, Russia.; Institute of Natural Sciences and Mathematics, Ural Federal University, 620000 Yekaterinburg, Russia., Perekucha NA; Siberian State Medical University, 634050 Tomsk, Russia., Romashchenko AV; Institute of Cytology and Genetics, Russian Academy of Sciences (Siberian Branch), 630090 Novosibirsk, Russia., Shevelev OB; Institute of Cytology and Genetics, Russian Academy of Sciences (Siberian Branch), 630090 Novosibirsk, Russia., Uimin MA; Mikheev Institute of Metal Physics, Russian Academy of Sciences (Ural Branch), 620990 Yekaterinburg, Russia., Byzov IV; Mikheev Institute of Metal Physics, Russian Academy of Sciences (Ural Branch), 620990 Yekaterinburg, Russia., Malkeyeva D; Institute of Cytology and Genetics, Russian Academy of Sciences (Siberian Branch), 630090 Novosibirsk, Russia., Kiseleva E; Institute of Cytology and Genetics, Russian Academy of Sciences (Siberian Branch), 630090 Novosibirsk, Russia., Efimova LV; Siberian State Medical University, 634050 Tomsk, Russia., Vtorushin SV; Siberian State Medical University, 634050 Tomsk, Russia.; Cancer Research Institute, Tomsk National Research Medical Center, Russian Academy of Sciences, 634050 Tomsk, Russia., Ogorodova LM; Siberian State Medical University, 634050 Tomsk, Russia., Krasnov VP; Postovsky Institute of Organic Synthesis, Russian Academy of Sciences (Ural Branch), 620108 Yekaterinburg, Russia.
المصدر:	ACS applied materials & interfaces [ACS Appl Mater Interfaces] 2021 Aug 11; Vol. 13 (31), pp. 36800-36815. Date of Electronic Publication: 2021 Jul 29.
نوع المنشور:	Journal Article
اللغة:	English
بيانات الدورية:	Publisher: American Chemical Society Country of Publication: United States NLM ID: 101504991 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1944-8252 (Electronic) Linking ISSN: 19448244 NLM ISO Abbreviation: ACS Appl Mater Interfaces Subsets: MEDLINE
أسماء مطبوعة:	Original Publication: Washington, D.C. : American Chemical Society
مواضيع طبية MeSH:	Contrast Media/chemistry , Immobilized Proteins/chemistry , Magnetite Nanoparticles/chemistry , Neoplasms/diagnostic imaging , Peptides/*chemistry, Amino Acid Sequence ; Animals ; Cell Line, Tumor ; Contrast Media/toxicity ; Female ; Humans ; Hydrogen-Ion Concentration ; Immobilized Proteins/toxicity ; Magnetic Resonance Imaging ; Magnetite Nanoparticles/toxicity ; Mice, Inbred BALB C ; Peptides/toxicity ; Silicon Dioxide/chemistry ; Silicon Dioxide/toxicity ; Mice
مستخلص:	Magnetic Fe 3 O 4 nanoparticles (MNPs) are often used to design agents enhancing contrast in magnetic resonance imaging (MRI) that can be considered as one of the efficient methods for cancer diagnostics. At present, increasing the specificity of the MRI contrast agent accumulation in tumor tissues remains an open question and attracts the attention of a wide range of researchers. One of the modern methods for enhancing the efficiency of contrast agents is the use of molecules for tumor acidic microenvironment targeting, for example, pH-low insertion peptide (pHLIP). We designed novel organosilicon MNPs covered with poly(ethylene glycol) (PEG) and covalently modified by pHLIP. To study the specific features of the binding of pHLIP-modified MNPs to cells, we also obtained nanoconjugates with Cy5 fluorescent dye embedded in the SiO 2 shell. The nanoconjugates obtained were characterized by transmission electron microscopy (TEM), attenuated total reflection (ATR), diffuse reflectance infrared Fourier transform spectroscopy (DRIFTS), dynamic light scattering (DLS), UV and fluorescence spectrometry, thermogravimetric analysis (TGA), CHN elemental analyses, and vibrating sample magnetometry. Low cytotoxicity and high specificity of cellular uptake of pHLIP-modified MNPs at pH 6.4 versus 7.4 (up to 23-fold) were demonstrated in vitro. The dynamics of the nanoconjugate accumulation in the 4T1 breast cancer orthotopically grown in BALB/c mice and MDA-MB231 xenografts was evaluated in MRI experiments. Biodistribution and biocompatibility studies of the obtained nanoconjugate showed no pathological change in organs and in the blood biochemical parameters of mice after MNP administration. A high accumulation rate of pHLIP-modified MNPs in tumor compared with PEGylated MNPs after their intravenous administration was demonstrated. Thus, we propose a promising approach to design an MRI agent with the tumor acidic microenvironment targeting ability.
فهرسة مساهمة:	Keywords: Fe3O4; MRI; magnetic nanoparticles; pH-triggered delivery; pHLIP; tumor
المشرفين على المادة:	0 (Contrast Media) 0 (Immobilized Proteins) 0 (Magnetite Nanoparticles) 0 (Peptides) 7631-86-9 (Silicon Dioxide)
تواريخ الأحداث:	Date Created: 20210729 Date Completed: 20211215 Latest Revision: 20240226
رمز التحديث:	20240226
DOI:	10.1021/acsami.1c07748
PMID:	34324807
قاعدة البيانات:	MEDLINE

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الوصف
تدمد:	1944-8252
DOI:	10.1021/acsami.1c07748