دورية أكاديمية
Assessment of escape from X chromosome inactivation and gene expression in single human immune cells.
| العنوان: | Assessment of escape from X chromosome inactivation and gene expression in single human immune cells. |
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| المؤلفون: | Hagen SH; Research Department Virus Immunology, Heinrich Pette Institute, Leibniz Institute for Experimental Virology, Martinistrasse 52, Hamburg 20251, Germany., Hennesen J; Technology Platform Flow Cytometry / FACS, Heinrich Pette Institute, Leibniz Institute for Experimental Virology, Martinistrasse 52, Hamburg 20251, Germany., Altfeld M; Research Department Virus Immunology, Heinrich Pette Institute, Leibniz Institute for Experimental Virology, Martinistrasse 52, Hamburg 20251, Germany. |
| المصدر: | STAR protocols [STAR Protoc] 2021 Jul 20; Vol. 2 (3), pp. 100641. Date of Electronic Publication: 2021 Jul 20 (Print Publication: 2021). |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Cell Press Country of Publication: United States NLM ID: 101769501 Publication Model: eCollection Cited Medium: Internet ISSN: 2666-1667 (Electronic) Linking ISSN: 26661667 NLM ISO Abbreviation: STAR Protoc |
| أسماء مطبوعة: | Original Publication: [Cambridge, MA] : Cell Press, [2020]- |
| مواضيع طبية MeSH: | Gene Expression* , X Chromosome Inactivation*, Leukocytes, Mononuclear/*metabolism, Alleles ; Cohort Studies ; DNA Primers ; Flow Cytometry ; Humans ; Polymorphism, Single Nucleotide ; Single-Cell Analysis/methods |
| مستخلص: | X-chromosomal genes escaping from X chromosome inactivation (XCI) in immune cells can contribute to sex-specific differences in immune responses. This protocol describes the specific steps to determine escape from XCI and to simultaneously quantify mRNA expression of multiple genes at the single immune cell level using a single-nucleotide polymorphism approach. The protocol furthermore allows the analysis of allele-specific expression of X-chromosomal genes. For complete details on the use and execution of this protocol, please refer to Hagen et al. (2020). Competing Interests: The authors declare no competing interests. (© 2021 The Authors.) |
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| فهرسة مساهمة: | Keywords: Flow Cytometry/Mass Cytometry; Gene Expression; Immunology; Molecular Biology |
| المشرفين على المادة: | 0 (DNA Primers) |
| تواريخ الأحداث: | Date Created: 20210806 Date Completed: 20220322 Latest Revision: 20220322 |
| رمز التحديث: | 20240829 |
| مُعرف محوري في PubMed: | PMC8319808 |
| DOI: | 10.1016/j.xpro.2021.100641 |
| PMID: | 34355200 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 2666-1667 |
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| DOI: | 10.1016/j.xpro.2021.100641 |