دورية أكاديمية
أعرض في EDS

In silico stress fibre content affects peak strain in cytoplasm and nucleus but not in the membrane for uniaxial substrate stretch.

التفاصيل البيبلوغرافية
العنوان: In silico stress fibre content affects peak strain in cytoplasm and nucleus but not in the membrane for uniaxial substrate stretch.
المؤلفون: Abdalrahman T; Division of Biomedical Engineering, Department of Human Biology, Faculty of Health Sciences, University of Cape Town, Observatory, South Africa.; Julius Wolff Institute for Biomechanics and Musculoskeletal Regeneration, Charité - Universitätsmedizin Berlin, Berlin, Germany., Davies NH; Chris Barnard Division of Cardiothoracic Surgery, Faculty of Health Sciences, University of Cape Town, Observatory, South Africa., Franz T; Division of Biomedical Engineering, Department of Human Biology, Faculty of Health Sciences, University of Cape Town, Observatory, South Africa. thomas.franz@uct.ac.za.; Bioengineering Science Research Group, University of Southampton, Southampton, UK. thomas.franz@uct.ac.za.
المصدر: Medical & biological engineering & computing [Med Biol Eng Comput] 2021 Sep; Vol. 59 (9), pp. 1933-1944. Date of Electronic Publication: 2021 Aug 14.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Springer Country of Publication: United States NLM ID: 7704869 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1741-0444 (Electronic) Linking ISSN: 01400118 NLM ISO Abbreviation: Med Biol Eng Comput Subsets: MEDLINE
أسماء مطبوعة: Publication: New York, NY : Springer
Original Publication: Stevenage, Eng., Peregrinus.
مواضيع طبية MeSH: Cell Nucleus* , Stress Fibers*, Computer Simulation ; Cytoplasm ; Humans ; Stress, Mechanical
مستخلص: Existing in silico models for single cell mechanics feature limited representations of cytoskeletal structures that contribute substantially to the mechanics of a cell. We propose a micromechanical hierarchical approach to capture the mechanical contribution of actin stress fibres. For a cell-specific fibroblast geometry with membrane, cytoplasm and nucleus, the Mori-Tanaka homogenization method was employed to describe cytoplasmic inhomogeneities and constitutive contribution of actin stress fibres. The homogenization was implemented in a finite element model of the fibroblast attached to a substrate through focal adhesions. Strain in cell membrane, cytoplasm and nucleus due to uniaxial substrate stretch was assessed for different stress fibre volume fractions and different elastic modulus of the substrate. A considerable decrease of the peak strain with increasing stress fibre content was observed in cytoplasm and nucleus but not the membrane, whereas the peak strain in cytoplasm, nucleus and membrane increased for increasing elastic modulus of the substrate. Finite element mesh of reconstructed human fibroblast and intracellular strain distribution in cell subjected to substrate stretch.
(© 2021. International Federation for Medical and Biological Engineering.)
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معلومات مُعتمدة: UID 92531 National Research Foundation (ZA); UID 93542 National Research Foundation (ZA); SIR 328148 South African Medical Research Council
فهرسة مساهمة: Keywords: Cell mechanics; Cytoskeleton; Micromechanical homogenization; Mori-Tanaka scheme
تواريخ الأحداث: Date Created: 20210815 Date Completed: 20210929 Latest Revision: 20210929
رمز التحديث: 20240628
DOI: 10.1007/s11517-021-02393-z
PMID: 34392447
قاعدة البيانات: MEDLINE
الوصف
تدمد:1741-0444
DOI:10.1007/s11517-021-02393-z