دورية أكاديمية
FGF18 modulates CTGF mRNA expression in cumulus-oocyte complexes and early bovine embryos: preliminary data.
| العنوان: | FGF18 modulates CTGF mRNA expression in cumulus-oocyte complexes and early bovine embryos: preliminary data. |
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| المؤلفون: | Silva ESD; Biotechnology and Animal Reproduction Laboratory, Universidade Federal de Santa Maria, Santa Maria, RS, 97105-900, Brazil., Amaral C; Biotechnology and Animal Reproduction Laboratory, Universidade Federal de Santa Maria, Santa Maria, RS, 97105-900, Brazil., Barreta M; Laboratory of Animal Reproduction Physiology, Universidade Federal de Santa Catarina, SC, 89520-000, Brazil., Antoniazzi A; Biotechnology and Animal Reproduction Laboratory, Universidade Federal de Santa Maria, Santa Maria, RS, 97105-900, Brazil., Andrade LG; Molecular and Integrative Physiology of Reproduction Laboratory, Universidade Federal do Pampa, Uruguaiana, RS, 97501-970, Brazil., Ferreira R; Department of Animal Science, Universidade do Estado de Santa Catarina, SC, 88035-901, Brazil., Mesquita F; Molecular and Integrative Physiology of Reproduction Laboratory, Universidade Federal do Pampa, Uruguaiana, RS, 97501-970, Brazil., Portela VM; Biotechnology and Animal Reproduction Laboratory, Universidade Federal de Santa Maria, Santa Maria, RS, 97105-900, Brazil., Gonçalves PB; Biotechnology and Animal Reproduction Laboratory, Universidade Federal de Santa Maria, Santa Maria, RS, 97105-900, Brazil.; Laboratory of Animal Reproduction Physiology, Universidade Federal de Santa Catarina, SC, 89520-000, Brazil.; Department of Animal Science, Universidade do Estado de Santa Catarina, SC, 88035-901, Brazil.; Molecular and Integrative Physiology of Reproduction Laboratory, Universidade Federal do Pampa, Uruguaiana, RS, 97501-970, Brazil. |
| المصدر: | Zygote (Cambridge, England) [Zygote] 2022 Apr; Vol. 30 (2), pp. 239-243. Date of Electronic Publication: 2021 Aug 18. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Cambridge University Press Country of Publication: England NLM ID: 9309124 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1469-8730 (Electronic) Linking ISSN: 09671994 NLM ISO Abbreviation: Zygote Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Cambridge ; New York, NY : Cambridge University Press, c1993- |
| مواضيع طبية MeSH: | Cumulus Cells* , In Vitro Oocyte Maturation Techniques*/veterinary, Animals ; Blastocyst ; Cattle ; Embryonic Development ; Female ; Fibroblast Growth Factors ; Oocytes ; Pregnancy ; Preliminary Data ; RNA, Messenger/genetics ; RNA, Messenger/metabolism |
| مستخلص: | The Hippo pathway is involved in the proliferation of intrafollicular cells and in early embryonic development, mainly because effectors of this pathway are key transcription regulators of genes such as CTGF and CYR61, which are involved in cell proliferation. Recent studies by our group found that fibroblast growth factor 18 (FGF18) is present in the fallopian tube during early embryonic development, leading to the hypothesis that FGF18 may have a role during embryonic development. Therefore, the aim of the following study was to determine whether FGF18 modulates the expression of Hippo pathway target genes, CTGF and CYR61, during oocyte maturation and early embryonic development. Three experiments were carried out, with in vitro maturation (IVM) of cumulus-oocyte complexes (COCs) and embryo culture. In experiment one, FGF18 (100 ng/ml) induced an increase (P < 0.05) in CTGF gene expression at 12 h post-exposure. In experiment two, FGF18 (100 ng/ml) induced a reduction (P < 0.05) in CTGF expression at 3 h post-exposure. In the third experiment, day 7 embryos exposed to FGF18 during oocyte IVM expressed greater CTGF mRNA abundance, whereas FGF18 exposure during embryo in vitro embryo culture did not alter CTGF expression in comparison with untreated controls. The preliminary data presented here show that FGF18 modulates CTGF expression in critical periods of oocyte nuclear maturation, cumulus expansion and early embryonic development in cattle. |
| فهرسة مساهمة: | Keywords: Bovine; Cell proliferation genes; Embryonic development; Fibroblast growth factor 18; Hippo Pathway |
| المشرفين على المادة: | 0 (RNA, Messenger) 0 (fibroblast growth factor 18) 62031-54-3 (Fibroblast Growth Factors) |
| تواريخ الأحداث: | Date Created: 20210818 Date Completed: 20220328 Latest Revision: 20220401 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1017/S0967199421000599 |
| PMID: | 34405788 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1469-8730 |
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| DOI: | 10.1017/S0967199421000599 |