دورية أكاديمية
One-shot identification of SARS-CoV-2 S RBD escape mutants using yeast screening.
| العنوان: | One-shot identification of SARS-CoV-2 S RBD escape mutants using yeast screening. |
|---|---|
| المؤلفون: | Francino-Urdaniz IM; Department of Chemical and Biological Engineering, University of Colorado, Boulder, CO 80305, USA., Steiner PJ; Department of Chemical and Biological Engineering, University of Colorado, Boulder, CO 80305, USA., Kirby MB; Department of Chemical and Biological Engineering, University of Colorado, Boulder, CO 80305, USA., Zhao F; The Scripps Research Institute, La Jolla, CA, USA., Haas CM; Department of Chemical and Biological Engineering, University of Colorado, Boulder, CO 80305, USA., Barman S; The Scripps Research Institute, La Jolla, CA, USA., Rhodes ER; Department of Chemical and Biological Engineering, University of Colorado, Boulder, CO 80305, USA., Leonard AC; Department of Chemical and Biological Engineering, University of Colorado, Boulder, CO 80305, USA., Peng L; The Scripps Research Institute, La Jolla, CA, USA., Sprenger KG; Department of Chemical and Biological Engineering, University of Colorado, Boulder, CO 80305, USA., Jardine JG; International AIDS Vaccine Initiative, New York, NY, USA., Whitehead TA; Department of Chemical and Biological Engineering, University of Colorado, Boulder, CO 80305, USA. Electronic address: timothy.whitehead@colorado.edu. |
| المصدر: | Cell reports [Cell Rep] 2021 Aug 31; Vol. 36 (9), pp. 109627. Date of Electronic Publication: 2021 Aug 10. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Cell Press Country of Publication: United States NLM ID: 101573691 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2211-1247 (Electronic) NLM ISO Abbreviation: Cell Rep Subsets: PubMed not MEDLINE; MEDLINE |
| أسماء مطبوعة: | Original Publication: [Cambridge, MA] : Cell Press, c 2012- |
| مستخلص: | The potential emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike (S) escape mutants is a threat to the efficacy of existing vaccines and neutralizing antibody (nAb) therapies. An understanding of the antibody/S escape mutation landscape is urgently needed to preemptively address this threat. Here we describe a rapid method to identify escape mutants for nAbs targeting the S receptor binding site. We identified escape mutants for five nAbs, including three from the public germline class VH3-53 elicited by natural coronavirus disease 2019 (COVID-19) infection. Escape mutations predominantly mapped to the periphery of the angiotensin-converting enzyme 2 (ACE2) recognition site on the RBD with K417, D420, Y421, F486, and Q493 as notable hotspots. We provide libraries, methods, and software as an openly available community resource to accelerate new therapeutic strategies against SARS-CoV-2. Competing Interests: Declaration of interests I.M.F.-U., P.J.S., M.B.K., C.M.H., J.G.J., and T.A.W. have a US provisional patent application “IDENTIFICATION OF SARS-COV-2 S RBD ESCAPE MUTANTS” covering some of the mutants identified in the present study. (Copyright © 2021 The Author(s). Published by Elsevier Inc. All rights reserved.) |
| التعليقات: | Update of: bioRxiv. 2021 Mar 15;:. (PMID: 33758848) |
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| معلومات مُعتمدة: | R01 AI141452 United States AI NIAID NIH HHS; T32 GM065103 United States GM NIGMS NIH HHS |
| فهرسة مساهمة: | Keywords: S RBD; SARS-CoV-2; deep mutational scanning; escape mutants; neutralizing antibodies; yeast surface display |
| تواريخ الأحداث: | Date Created: 20210820 Latest Revision: 20231107 |
| رمز التحديث: | 20240628 |
| مُعرف محوري في PubMed: | PMC8352667 |
| DOI: | 10.1016/j.celrep.2021.109627 |
| PMID: | 34416153 |
| قاعدة البيانات: | MEDLINE |
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