دورية أكاديمية
أعرض في EDS

Human adipose and skeletal muscle tissue DNA, RNA, and protein content.

التفاصيل البيبلوغرافية
العنوان: Human adipose and skeletal muscle tissue DNA, RNA, and protein content.
المؤلفون: Stroh AM; Human Performance Laboratory, Ball State University, Muncie, Indiana., Lynch CE; Human Performance Laboratory, Ball State University, Muncie, Indiana., Lester BE; Human Performance Laboratory, Ball State University, Muncie, Indiana., Minchev K; Human Performance Laboratory, Ball State University, Muncie, Indiana., Chambers TL; Human Performance Laboratory, Ball State University, Muncie, Indiana., Montenegro CF; Human Performance Laboratory, Ball State University, Muncie, Indiana., Chavez Martinez C; Human Performance Laboratory, Ball State University, Muncie, Indiana., Fountain WA; Human Performance Laboratory, Ball State University, Muncie, Indiana., Trappe TA; Human Performance Laboratory, Ball State University, Muncie, Indiana., Trappe SW; Human Performance Laboratory, Ball State University, Muncie, Indiana.
المصدر: Journal of applied physiology (Bethesda, Md. : 1985) [J Appl Physiol (1985)] 2021 Oct 01; Vol. 131 (4), pp. 1370-1379. Date of Electronic Publication: 2021 Aug 26.
نوع المنشور: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: American Physiological Society Country of Publication: United States NLM ID: 8502536 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1522-1601 (Electronic) Linking ISSN: 01617567 NLM ISO Abbreviation: J Appl Physiol (1985) Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Bethesda, MD : American Physiological Society, c1985-
مواضيع طبية MeSH: Muscle, Skeletal* , RNA*, Adipose Tissue ; DNA ; Exercise ; Humans
مستخلص: The purpose of this project was to provide a profile of DNA, RNA, and protein content in adipose tissue, which is relatively understudied in humans, to gain more insight into the amount of tissue that may be required for various analyses. Skeletal muscle tissue was also investigated to provide a direct comparison into potential differences between these two highly metabolically active tissues. Basal adipose and skeletal muscle tissue samples were obtained from 10 (7 M, 3 W) recreationally active participants [25 ± 1 yr; 84 ± 3 kg, maximal oxygen consumption (V̇o 2max ): 3.5 ± 0.2 L/min, body fat: 29 ± 2%]. DNA, RNA, and protein were extracted and subsequently analyzed for quantity and quality. DNA content of adipose and skeletal muscle tissue was 52 ± 14 and 189 ± 44 ng DNA·mg tissue -1 , respectively ( P < 0.05). RNA content of adipose and skeletal muscle tissue was 46 ± 14 and 537 ± 72 ng RNA·mg tissue -1 , respectively ( P < 0.05). Protein content of adipose and skeletal muscle tissue was 4 ± 1 and 177 ± 10 µg protein·mg tissue -1 , respectively ( P < 0.05). In summary, human adipose had 28% of the DNA, 9% of the RNA, and 2% of the protein found in skeletal muscle per mg of tissue. This information should be useful across a wide range of human clinical investigation designs and various laboratory analyses. NEW & NOTEWORTHY This investigation studied DNA, RNA, and protein contents of adipose and skeletal muscle tissues from young active individuals. A series of optimization steps were investigated to aid in determining the optimal approach to extract high-yield and high-quality biomolecules. These findings contribute to the knowledge gap in adipose tissue requirements for molecular biology assays, which is of increasing importance due to the growing interest in adipose tissue research involving human exercise physiology research.
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معلومات مُعتمدة: U01 AR071133 United States AR NIAMS NIH HHS; U01AR071133 United States NH NIH HHS
فهرسة مساهمة: Keywords: DNA; RNA; adipose; protein; skeletal muscle
المشرفين على المادة: 63231-63-0 (RNA)
9007-49-2 (DNA)
تواريخ الأحداث: Date Created: 20210826 Date Completed: 20211029 Latest Revision: 20221003
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC8560392
DOI: 10.1152/japplphysiol.00343.2021
PMID: 34435508
قاعدة البيانات: MEDLINE
الوصف
تدمد:1522-1601
DOI:10.1152/japplphysiol.00343.2021