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PDT with genetically encoded photosensitizer miniSOG on a tumor spheroid model: A comparative study of continuous-wave and pulsed irradiation.

التفاصيل البيبلوغرافية
العنوان: PDT with genetically encoded photosensitizer miniSOG on a tumor spheroid model: A comparative study of continuous-wave and pulsed irradiation.
المؤلفون: Yuzhakova DV; Privolzhsky Research Medical University, 10/1 Minin and Pozharsky Sq., 603005 Nizhny Novgorod, Russia. Electronic address: yuzhakova-diana@mail.ru., Shirmanova MV; Privolzhsky Research Medical University, 10/1 Minin and Pozharsky Sq., 603005 Nizhny Novgorod, Russia., Klimenko VV; Saint-Petersburg Clinical Scientific and Practical Center of Specialized Types of Medical Care (Oncological), 68A Leningradskaya St., Pesochny Settlement, 197758 St. Petersburg, Russia., Lukina MM; Privolzhsky Research Medical University, 10/1 Minin and Pozharsky Sq., 603005 Nizhny Novgorod, Russia., Gavrina AI; Privolzhsky Research Medical University, 10/1 Minin and Pozharsky Sq., 603005 Nizhny Novgorod, Russia., Komarova AD; Privolzhsky Research Medical University, 10/1 Minin and Pozharsky Sq., 603005 Nizhny Novgorod, Russia., Gorbachev DA; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, 16/10 Miklukho-Maklaya St., GSP-7, 117997 Moscow, Russia., Sapogova NV; Institute of Applied Physics of the Russian Academy of Sciences, 46 Ulyanov St., 603950 Nizhny Novgorod, Russia., Lukyanov KA; Center of Life Sciences, Skolkovo Institute of Science and Technology, 30/1 Bolshoy Boulevard, 121205 Moscow, Russia., Kamensky VA; Institute of Applied Physics of the Russian Academy of Sciences, 46 Ulyanov St., 603950 Nizhny Novgorod, Russia.
المصدر: Biochimica et biophysica acta. General subjects [Biochim Biophys Acta Gen Subj] 2021 Dec; Vol. 1865 (12), pp. 129978. Date of Electronic Publication: 2021 Sep 03.
نوع المنشور: Journal Article; Comparative Study
اللغة: English
بيانات الدورية: Publisher: Elsevier Country of Publication: Netherlands NLM ID: 101731726 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1872-8006 (Electronic) Linking ISSN: 03044165 NLM ISO Abbreviation: Biochim Biophys Acta Gen Subj
أسماء مطبوعة: Original Publication: Amsterdam : Elsevier
مواضيع طبية MeSH: Photosensitizing Agents*/pharmacology , Spheroids, Cellular*/drug effects , Spheroids, Cellular*/radiation effects , Spheroids, Cellular*/pathology , Photochemotherapy*/methods, Humans ; HeLa Cells ; Singlet Oxygen/metabolism ; Apoptosis/drug effects ; Apoptosis/radiation effects ; Flavin Mononucleotide
مستخلص: Background: Therapeutic effects of PDT depend on many factors, including the amount of singlet oxygen, localization of photosensitizer and irradiation protocol. The present study was aimed to compare the cytotoxic mechanisms of PDT under continuous-wave (CW) and pulsed irradiation using a tumor spheroid model and a genetically encoded photosensitizer miniSOG.
Methods: 1 O 2 detection in miniSOG and flavin mononucleotide (FMN) solutions was performed. Photobleaching of miniSOG in solution and in HeLa tumor spheroids was analyzed. Tumor spheroid morphology and growth and the cell death mechanisms after PDT in CW and pulsed modes were assessed.
Results: We found a more rapid 1 O 2 generation and a higher photobleaching rate in miniSOG solution upon irradiation in pulsed mode compared to CW mode. Photobleaching of miniSOG in tumor spheroids was also higher after irradiation in the pulsed mode. PDT of spheroids in CW mode resulted in a moderate expansion of the necrotic core of tumor spheroids and a slight inhibition of spheroid growth. The pulsed mode was more effective in induction of cell death, including apoptosis, and suppression of spheroid growth.
Conclusions: Comparison of CW and pulsed irradiation modes in PDT with miniSOG showed more pronounced cytotoxic effects of the pulsed mode. Our results suggest that the pulsed irradiation regimen enables enhanced 1 O 2 production by photosensitizer and stimulates apoptosis.
General Significance: Our results provide more insights into the cellular mechanisms of anti-cancer PDT and open the way to improvement of light irradiation protocols.
(Copyright © 2021 Elsevier B.V. All rights reserved.)
فهرسة مساهمة: Keywords: 3D tumor spheroid model; Continuous-wave irradiation; Fluorescence imaging; PDT; Pulsed irradiation; miniSOG
المشرفين على المادة: 0 (Photosensitizing Agents)
17778-80-2 (Singlet Oxygen)
7N464URE7E (Flavin Mononucleotide)
تواريخ الأحداث: Date Created: 20210906 Date Completed: 20240724 Latest Revision: 20240724
رمز التحديث: 20240726
DOI: 10.1016/j.bbagen.2021.129978
PMID: 34487824
قاعدة البيانات: MEDLINE
الوصف
تدمد:1872-8006
DOI:10.1016/j.bbagen.2021.129978