دورية أكاديمية
A Novel Xenograft Model Demonstrates Human Fibroblast Behavior During Skin Wound Repair and Fibrosis.
| العنوان: | A Novel Xenograft Model Demonstrates Human Fibroblast Behavior During Skin Wound Repair and Fibrosis. |
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| المؤلفون: | Borrelli MR; Division of Plastic and Reconstructive Surgery, Department of Surgery, Stanford University School of Medicine, Stanford, California, USA., Shen AH; Division of Plastic and Reconstructive Surgery, Department of Surgery, Stanford University School of Medicine, Stanford, California, USA., Griffin M; Division of Plastic and Reconstructive Surgery, Department of Surgery, Stanford University School of Medicine, Stanford, California, USA., Mascharak S; Division of Plastic and Reconstructive Surgery, Department of Surgery, Stanford University School of Medicine, Stanford, California, USA., Adem S; Division of Plastic and Reconstructive Surgery, Department of Surgery, Stanford University School of Medicine, Stanford, California, USA., Deleon NMD; Division of Plastic and Reconstructive Surgery, Department of Surgery, Stanford University School of Medicine, Stanford, California, USA., Ngaage LM; Division of Plastic and Reconstructive Surgery, Department of Surgery, Stanford University School of Medicine, Stanford, California, USA., Longaker MT; Division of Plastic and Reconstructive Surgery, Department of Surgery, Stanford University School of Medicine, Stanford, California, USA.; Department of Surgery, Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, Stanford, California, USA., Wan DC; Division of Plastic and Reconstructive Surgery, Department of Surgery, Stanford University School of Medicine, Stanford, California, USA., Lorenz HP; Division of Plastic and Reconstructive Surgery, Department of Surgery, Stanford University School of Medicine, Stanford, California, USA. |
| المصدر: | Advances in wound care [Adv Wound Care (New Rochelle)] 2022 Sep; Vol. 11 (9), pp. 455-465. Date of Electronic Publication: 2021 Oct 26. |
| نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Mary Ann Liebert, Inc Country of Publication: United States NLM ID: 101590593 Publication Model: Print-Electronic Cited Medium: Print ISSN: 2162-1918 (Print) Linking ISSN: 21621918 NLM ISO Abbreviation: Adv Wound Care (New Rochelle) Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: New Rochelle, N.Y. : Mary Ann Liebert, Inc. |
| مواضيع طبية MeSH: | Fibroblast Growth Factor 2* , Soft Tissue Injuries*, Animals ; Cicatrix ; Collagen ; Disease Models, Animal ; Fibroblasts ; Fibrosis ; Heterografts ; Humans ; Mice ; Mice, SCID |
| مستخلص: | Objective: Xenografts of human skin in immunodeficient mice provide a means of assessing human skin physiology and its response to wounding. Approach: We describe a novel xenograft model using full-thickness human neonatal foreskin to examine human skin wound repair. Full-thickness 8 mm human neonatal foreskin biopsies were sutured into the dorsum of NOD scid gamma (NSG; NOD.Cg-Prkdc scid Il2rg tm1Wjl /SzJ) pups as subcutaneous grafts. At postnatal day 21 the subcutaneous grafts were exposed to cutaneous grafts. Following maturation of 2 months, xenografts were then wounded with 5 mm linear incisions and monitored until postwound day (PWD) 14 to study skin repair and fibrosis. To explore whether our model can be used to test the efficacy of topical therapies, wounded xenografts were injected with antifibrotic fibroblast growth factor 2 (FGF2) for the first four consecutive PWDs. Xenografts were harvested for analysis by histology and fluorescence-activated cell sorting (FACS). Results: Xenografts were successfully engrafted with evidence of mouse-human anastomoses and resembled native neonatal foreskin at the gross and microscopic level. Wounded xenografted skin scarred with human collagen and an expansion of CD26-positive human fibroblasts. Collagen scar was quantitated by neural network analysis, which revealed distinct clustering of collagen fiber networks from unwounded skin and wounded skin at PWD7 and PWD14. Collagen fiber networks within FGF2-treated wounds at PWD14 resembled those in untreated wounded xenografts at PWD7, suggesting that FGF2 treatment at time of wounding can reduce fibrosis. Innovation and Conclusion: This novel xenograft model can be used to investigate acute fibrosis, fibroblast heterogeneity, and the efficacy of antifibrotic agents during wound repair in human skin. |
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| معلومات مُعتمدة: | R01 DE027346 United States DE NIDCR NIH HHS; R01 GM116892 United States GM NIGMS NIH HHS; U01 HL099776 United States HL NHLBI NIH HHS |
| فهرسة مساهمة: | Keywords: fibroblast heterogeneity; fibrosis; scarring; xenograft |
| المشرفين على المادة: | 103107-01-3 (Fibroblast Growth Factor 2) 9007-34-5 (Collagen) |
| تواريخ الأحداث: | Date Created: 20210915 Date Completed: 20220620 Latest Revision: 20230902 |
| رمز التحديث: | 20230902 |
| مُعرف محوري في PubMed: | PMC9245791 |
| DOI: | 10.1089/wound.2020.1392 |
| PMID: | 34521222 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 2162-1918 |
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| DOI: | 10.1089/wound.2020.1392 |