دورية أكاديمية
An alternative, zeaxanthin epoxidase-independent abscisic acid biosynthetic pathway in plants.
| العنوان: | An alternative, zeaxanthin epoxidase-independent abscisic acid biosynthetic pathway in plants. |
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| المؤلفون: | Jia KP; Biological and Environmental Sciences and Engineering Division, Center for Desert Agriculture, The BioActives Lab, King Abdullah University of Science and Technology, Thuwal 23955-6900, Saudi Arabia; State Key Laboratory of Cotton Biology, Henan Joint International Laboratory for Crop Multi-Omics Research, School of Life Sciences, Henan University, Jinming Road, Kaifeng 475004, China., Mi J; Biological and Environmental Sciences and Engineering Division, Center for Desert Agriculture, The BioActives Lab, King Abdullah University of Science and Technology, Thuwal 23955-6900, Saudi Arabia., Ali S; Biological and Environmental Sciences and Engineering Division, Center for Desert Agriculture, The BioActives Lab, King Abdullah University of Science and Technology, Thuwal 23955-6900, Saudi Arabia., Ohyanagi H; Biological and Environmental Sciences and Engineering Division, Computational Bioscience Research Center, King Abdullah University of Science and Technology, Thuwal 23955-6900, Saudi Arabia., Moreno JC; Biological and Environmental Sciences and Engineering Division, Center for Desert Agriculture, The BioActives Lab, King Abdullah University of Science and Technology, Thuwal 23955-6900, Saudi Arabia., Ablazov A; Biological and Environmental Sciences and Engineering Division, Center for Desert Agriculture, The BioActives Lab, King Abdullah University of Science and Technology, Thuwal 23955-6900, Saudi Arabia., Balakrishna A; Biological and Environmental Sciences and Engineering Division, Center for Desert Agriculture, The BioActives Lab, King Abdullah University of Science and Technology, Thuwal 23955-6900, Saudi Arabia., Berqdar L; Biological and Environmental Sciences and Engineering Division, Center for Desert Agriculture, The BioActives Lab, King Abdullah University of Science and Technology, Thuwal 23955-6900, Saudi Arabia., Fiore A; Italian National Agency for New Technologies, Energy and Sustainable Economic Development, 00123 Rome, Italy., Diretto G; Italian National Agency for New Technologies, Energy and Sustainable Economic Development, 00123 Rome, Italy., Martínez C; Universidade de Vigo, Facultade de Química and CINBIO, 36310 Vigo, Spain., de Lera AR; Universidade de Vigo, Facultade de Química and CINBIO, 36310 Vigo, Spain., Gojobori T; Biological and Environmental Sciences and Engineering Division, Computational Bioscience Research Center, King Abdullah University of Science and Technology, Thuwal 23955-6900, Saudi Arabia., Al-Babili S; Biological and Environmental Sciences and Engineering Division, Center for Desert Agriculture, The BioActives Lab, King Abdullah University of Science and Technology, Thuwal 23955-6900, Saudi Arabia. Electronic address: salim.babili@kaust.edu.sa. |
| المصدر: | Molecular plant [Mol Plant] 2022 Jan 03; Vol. 15 (1), pp. 151-166. Date of Electronic Publication: 2021 Sep 20. |
| نوع المنشور: | Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Cell Press Country of Publication: England NLM ID: 101465514 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1752-9867 (Electronic) Linking ISSN: 16742052 NLM ISO Abbreviation: Mol Plant Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2015- : Cambridge, MA Cell Press Original Publication: Oxford, England : Oxford University Press, 2008- |
| مواضيع طبية MeSH: | Biosynthetic Pathways*, Abscisic Acid/*metabolism , Arabidopsis/*genetics , Arabidopsis/*metabolism , Gene Expression Regulation, Plant/*drug effects , Oxidoreductases/*genetics , Oxidoreductases/*metabolism, Genes, Plant ; Genetic Variation ; Genotype |
| مستخلص: | Abscisic acid (ABA) is an important carotenoid-derived phytohormone that plays essential roles in plant response to biotic and abiotic stresses as well as in various physiological and developmental processes. In Arabidopsis, ABA biosynthesis starts with the epoxidation of zeaxanthin by the ABA DEFICIENT 1 (ABA1) enzyme, leading to epoxycarotenoids; e.g., violaxanthin. The oxidative cleavage of 9-cis-epoxycarotenoids, a key regulatory step catalyzed by 9-CIS-EPOXYCAROTENOID DIOXYGENASE, forms xanthoxin, which is converted in further reactions mediated by ABA DEFICIENT 2 (ABA2), ABA DEFICIENT 3 (ABA3), and ABSCISIC ALDEHYDE OXIDASE 3 (AAO3) into ABA. By combining genetic and biochemical approaches, we unravel here an ABA1-independent ABA biosynthetic pathway starting upstream of zeaxanthin. We identified the carotenoid cleavage products (i.e., apocarotenoids, β-apo-11-carotenal, 9-cis-β-apo-11-carotenal, 3-OH-β-apo-11-carotenal, and 9-cis-3-OH-β-apo-11-carotenal) as intermediates of this ABA1-independent ABA biosynthetic pathway. Using labeled compounds, we showed that β-apo-11-carotenal, 9-cis-β-apo-11-carotenal, and 3-OH-β-apo-11-carotenal are successively converted into 9-cis-3-OH-β-apo-11-carotenal, xanthoxin, and finally into ABA in both Arabidopsis and rice. When applied to Arabidopsis, these β-apo-11-carotenoids exert ABA biological functions, such as maintaining seed dormancy and inducing the expression of ABA-responsive genes. Moreover, the transcriptomic analysis revealed a high overlap of differentially expressed genes regulated by β-apo-11-carotenoids and ABA, suggesting that β-apo-11-carotenoids exert ABA-independent regulatory activities. Taken together, our study identifies a biological function for the common plant metabolites, β-apo-11-carotenoids, extends our knowledge about ABA biosynthesis, and provides new insights into plant apocarotenoid metabolic networks. (Copyright © 2021 The Authors. Published by Elsevier Inc. All rights reserved.) |
| فهرسة مساهمة: | Keywords: ABA; ABA biosynthesis; ABA1; Arabidopsis thaliana.; apocarotenoids; carotenoids; seed germination; ultra-high-performance liquid chromatography-mass spectrometry |
| المشرفين على المادة: | 72S9A8J5GW (Abscisic Acid) EC 1.- (Oxidoreductases) |
| تواريخ الأحداث: | Date Created: 20210921 Date Completed: 20220131 Latest Revision: 20220131 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1016/j.molp.2021.09.008 |
| PMID: | 34547513 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1752-9867 |
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| DOI: | 10.1016/j.molp.2021.09.008 |