دورية أكاديمية
Time Mating Guinea Pigs by Monitoring Changes to the Vaginal Membrane throughout the Estrus Cycle and with Ultrasound Confirmation.
| العنوان: | Time Mating Guinea Pigs by Monitoring Changes to the Vaginal Membrane throughout the Estrus Cycle and with Ultrasound Confirmation. |
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| المؤلفون: | Wilson RL; Center for Research in Perinatal Outcomes, College of Medicine, University of Florida, Gainesville, FL 32610, USA.; Department of Physiology and Functional Genomics, College of Medicine, University of Florida, Gainesville, FL 32610, USA., Lampe K; Center for Fetal and Placental Research, Cincinnati Children's Hospital and Medical Center, Cincinnati, OH 45229, USA., Matushewski BJ; Department of Kinesiology, Western University, London, ON N6A 5B9, Canada., Regnault TRH; Departments of Physiology and Pharmacology, and Obstetrics and Gynaecology, Schulich School of Medicine and Dentistry, Western University, London, ON N6A 5B9, Canada.; Children's Health Research Institute and Lawson Health Research Institute, London, ON N6A 5B9, Canada., Jones HN; Center for Research in Perinatal Outcomes, College of Medicine, University of Florida, Gainesville, FL 32610, USA.; Department of Physiology and Functional Genomics, College of Medicine, University of Florida, Gainesville, FL 32610, USA. |
| المصدر: | Methods and protocols [Methods Protoc] 2021 Aug 27; Vol. 4 (3). Date of Electronic Publication: 2021 Aug 27. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: MDPI AG Country of Publication: Switzerland NLM ID: 101720073 Publication Model: Electronic Cited Medium: Internet ISSN: 2409-9279 (Electronic) Linking ISSN: 24099279 NLM ISO Abbreviation: Methods Protoc Subsets: PubMed not MEDLINE |
| أسماء مطبوعة: | Original Publication: Basel, Switzerland : MDPI AG, [2018]- |
| مستخلص: | One of the greatest challenges to the development and implementation of pregnancy therapeutics is the ability to rigorously test treatments in clinically relevant animal models. Guinea pigs offer a unique advantage in studying the placenta, fetal development, and reproductive health as they have similar developmental milestones to humans, both throughout gestation and following birth. Tracking the guinea pig estrus cycle is imperative to ensuring appropriately timed mating and can be performed by monitoring the guinea pig vaginal membrane. Here, we describe a methodology to efficiently and accurately time mate guinea pigs, and provide a picture representation of changes to the guinea pig vaginal membrane throughout the estrus cycle. Utilization of this monitoring enabled a 100% pregnancy success rate on the first mating attempt in a cohort of five guinea pigs. This approach, along with early pregnancy ultrasounds as a secondary method to confirm pregnancy, offers a reliable approach to timed mating in the guinea pig. |
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| معلومات مُعتمدة: | R01HD090657 Eunice Kennedy Shriver National Institute of Child Health and Human Development |
| فهرسة مساهمة: | Keywords: guinea pig; methodology; pregnancy; reproduction; time mate |
| تواريخ الأحداث: | Date Created: 20210926 Latest Revision: 20211002 |
| رمز التحديث: | 20240628 |
| مُعرف محوري في PubMed: | PMC8482275 |
| DOI: | 10.3390/mps4030058 |
| PMID: | 34564304 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 2409-9279 |
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| DOI: | 10.3390/mps4030058 |