دورية أكاديمية
أعرض في EDS

Use of real-time PCR as an alternative to conventional genotyping methods for the laboratory detection of lymphogranuloma venereum (LGV).

التفاصيل البيبلوغرافية
العنوان: Use of real-time PCR as an alternative to conventional genotyping methods for the laboratory detection of lymphogranuloma venereum (LGV).
المؤلفون: Woodson EN; Laboratory Leadership Service, Centers for Disease Control and Prevention, Atlanta, GA, USA; Division of STD Prevention, National Center for HIV, Hepatitis, STD, TB Prevention, Centers for Disease Control and Prevention, Atlanta, GA, USA. Electronic address: phy2@cdc.gov., Katz SS; Division of STD Prevention, National Center for HIV, Hepatitis, STD, TB Prevention, Centers for Disease Control and Prevention, Atlanta, GA, USA., Mosley SS; Division of STD Prevention, National Center for HIV, Hepatitis, STD, TB Prevention, Centers for Disease Control and Prevention, Atlanta, GA, USA., Danavall DC; Division of STD Prevention, National Center for HIV, Hepatitis, STD, TB Prevention, Centers for Disease Control and Prevention, Atlanta, GA, USA., Bowden KE; Division of STD Prevention, National Center for HIV, Hepatitis, STD, TB Prevention, Centers for Disease Control and Prevention, Atlanta, GA, USA., Chi KH; Division of STD Prevention, National Center for HIV, Hepatitis, STD, TB Prevention, Centers for Disease Control and Prevention, Atlanta, GA, USA., Raphael BH; Division of STD Prevention, National Center for HIV, Hepatitis, STD, TB Prevention, Centers for Disease Control and Prevention, Atlanta, GA, USA.
المصدر: Diagnostic microbiology and infectious disease [Diagn Microbiol Infect Dis] 2021 Dec; Vol. 101 (4), pp. 115532. Date of Electronic Publication: 2021 Aug 27.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier Biomedical Country of Publication: United States NLM ID: 8305899 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0070 (Electronic) Linking ISSN: 07328893 NLM ISO Abbreviation: Diagn Microbiol Infect Dis Subsets: MEDLINE
أسماء مطبوعة: Original Publication: [New York, NY] : Elsevier Biomedical, [c1983-
مواضيع طبية MeSH: Chlamydia trachomatis/*isolation & purification , Lymphogranuloma Venereum/*diagnosis , Molecular Diagnostic Techniques/*methods , Real-Time Polymerase Chain Reaction/*methods, Bacterial Outer Membrane Proteins/genetics ; Chlamydia trachomatis/classification ; Chlamydia trachomatis/genetics ; DNA, Bacterial/genetics ; Genome, Bacterial/genetics ; Genotype ; Humans ; Lymphogranuloma Venereum/microbiology ; Molecular Diagnostic Techniques/standards ; Real-Time Polymerase Chain Reaction/standards ; Rectum/microbiology ; Reproducibility of Results ; Sensitivity and Specificity ; Sequence Analysis, DNA ; Serogroup
مستخلص: Lymphogranuloma venereum (LGV) can be differentiated from non-LGV chlamydial infection using Sanger sequencing or molecular assays, including those that are commercially-available internationally. Here, we describe the performance of a rapid real-time PCR (RT-PCR)-based strategy in differentiating Chlamydia trachomatis infections associated with LGV or non-LGV serovars. One hundred three rectal swabs, previously genotyped using Sanger sequencing of the ompA gene as a reference method, were tested in the RT-PCR assays. All non-LGV specimens were correctly identified, but the RT-PCR failed to detect 1 LGV specimen, resulting in a sensitivity of 87.5% for the non-LGV/LGV RT-PCR assay. Additional performance characteristics (e.g., specificity, accuracy, and reproducibility) were all between 93% and 100% with a limit of detection ≤100 copies/reaction. Thus, this rapid RT-PCR method for LGV detection in clinical specimens is comparable to the reference method.
Competing Interests: Declaration of competing interest The authors report no conflicts of interest relevant to this article.
(Published by Elsevier Inc.)
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معلومات مُعتمدة: CC999999 United States ImCDC Intramural CDC HHS
فهرسة مساهمة: Keywords: Chlamydia trachomatis; Lymphogranuloma venereum; Outer membrane protein A (ompA); Real-time PCR
المشرفين على المادة: 0 (Bacterial Outer Membrane Proteins)
0 (DNA, Bacterial)
149024-69-1 (OMPA outer membrane proteins)
تواريخ الأحداث: Date Created: 20210927 Date Completed: 20220131 Latest Revision: 20221202
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC8650105
DOI: 10.1016/j.diagmicrobio.2021.115532
PMID: 34571353
قاعدة البيانات: MEDLINE
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