دورية أكاديمية
Quercetin suppresses inflammatory cytokine production in rheumatoid arthritis fibroblast-like synoviocytes.
العنوان: | Quercetin suppresses inflammatory cytokine production in rheumatoid arthritis fibroblast-like synoviocytes. |
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المؤلفون: | Sun HT; Laboratory of New Techniques of Restoration and Reconstruction of Orthopedics and Traumatology, Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210023, P.R. China.; Department of Emergency Trauma Center, Wuxi Huishan District People's Hospital, Wuxi, Jiangsu 214187, P.R. China., Li JP; Department of Orthopedics, Tianjin Medical University, Tianjin 300070, P.R. China.; Department of Orthopedics, Tianjin Fifth Central Hospital, Tianjin 300450, P.R. China., Qian WQ; Department of Orthopedics, The Third Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210001, P.R. China., Yin MF; Department of Orthopedics, Tianjin Fifth Central Hospital, Tianjin 300450, P.R. China., Yin H; Department of Orthopedics, The Third Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210001, P.R. China., Huang GC; Laboratory of New Techniques of Restoration and Reconstruction of Orthopedics and Traumatology, The First Clinical Medical College, Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210023, P.R. China. |
المصدر: | Experimental and therapeutic medicine [Exp Ther Med] 2021 Nov; Vol. 22 (5), pp. 1260. Date of Electronic Publication: 2021 Sep 06. |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: Spandidos Pub.] Country of Publication: Greece NLM ID: 101531947 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1792-1015 (Electronic) Linking ISSN: 17920981 NLM ISO Abbreviation: Exp Ther Med Subsets: PubMed not MEDLINE |
أسماء مطبوعة: | Original Publication: [Athens : Spandidos Pub.] |
مستخلص: | Rheumatoid arthritis (RA) is a chronic, progressive and systemic autoimmune disease mainly characterized by symmetric multijoint synovitis. Quercetin has anti-inflammatory, anti-oxidation and immune regulation activities, and therefore shows high medicinal value. The present study aimed to observe the effect of quercetin on fibroblast-like synoviocytes (FLSs) in RA. Rheumatoid arthritis fibroblast-like synoviocytes (RAFLSs) were pretreated with 50 nmol/l quercetin for 2 h and were then stimulated using TNF-α for 24 h for subsequent experiments. RAFLSs were transfected with short interfering (si)-X-inactive specific transcript (XIST), microRNA (miR)-485 mimic, miR-485 inhibitor or si-PSMB8 or combination. ELISA, PCR and western blotting was used to evaluate the effect of quercetin on RAFLSs treated with TNF-α. It was revealed that quercetin inhibited the production of inflammatory cytokines and the expression of XIST in RAFLSs induced by TNF-α. Bioinformatics analysis indicated that XIST acted as a sponge for miR-485 and that proteasome subunit β type-8 (PSMB8) was a direct target of miR-485. Moreover, PSMB8 functioned as a suppressor in inflammatory cytokine production of RAFLSs induced by TNF-α. Overall, quercetin was observed to inhibit the production of inflammatory cytokines and the expression of XIST in RAFLSs induced by TNF-α. Moreover, XIST-silencing could suppress the inflammatory reaction by sponging miR-485 in cells treated with TNF-α. Altogether, quercetin could suppress the development of RA in vitro. Competing Interests: The authors declare that they have no competing interests. (Copyright: © Sun et al.) |
References: | Nat Struct Mol Biol. 2015 Jan;22(1):5-7. (PMID: 25565026) Clin Immunol. 2005 May;115(2):118-28. (PMID: 15885632) Nature. 2003 May 15;423(6937):356-61. (PMID: 12748655) Nat Prod Res. 2013;27(9):834-6. (PMID: 22799569) Nature. 1991 Jan 3;349(6304):38-44. (PMID: 1985261) Atherosclerosis. 2008 Mar;197(1):50-6. (PMID: 17880982) Lancet. 2016 Oct 22;388(10055):2023-2038. (PMID: 27156434) Nat Rev Genet. 2004 Jul;5(7):522-31. (PMID: 15211354) Oncotarget. 2017 Feb 7;8(31):50873-50888. (PMID: 28881611) Exp Gerontol. 2011 Apr;46(4):217-24. (PMID: 21111800) Nat Rev Mol Cell Biol. 2013 Nov;14(11):699-712. (PMID: 24105322) J Surg Res. 2020 Feb;246:213-223. (PMID: 31606511) Arthritis Rheum. 1996 Nov;39(11):1781-90. (PMID: 8912499) Arthritis Res Ther. 2009;11(3):229. (PMID: 19519924) Mayo Clin Proc. 2012 Jul;87(7):659-73. (PMID: 22766086) J Cell Biochem. 2018 Dec;119(12):9888-9898. (PMID: 30129228) Biochem Pharmacol. 2006 Nov 15;72(10):1304-10. (PMID: 16959220) Nat Prod Commun. 2010 Oct;5(10):1597-9. (PMID: 21121256) Arch Med Sci. 2018 Apr;14(3):569-571. (PMID: 29765444) Altern Med Rev. 2000 Jun;5(3):196-208. (PMID: 10869101) Nutrition. 2008 Jul-Aug;24(7-8):703-10. (PMID: 18549926) Arthritis Res Ther. 2017 May 31;19(1):110. (PMID: 28569176) Cell Biochem Funct. 2019 Jul;37(5):348-358. (PMID: 31066476) J Agric Food Chem. 2006 Dec 27;54(26):9966-77. (PMID: 17177529) J Pharm Sci. 2011 Jan;100(1):195-205. (PMID: 20607810) Lancet. 2009 Feb 21;373(9664):659-72. (PMID: 19157532) Methods. 2001 Dec;25(4):402-8. (PMID: 11846609) Tumour Biol. 2016 Jan;37(1):163-75. (PMID: 26586396) Arthritis Res Ther. 2005;7(1):R118-26. (PMID: 15642131) Int J Sport Nutr Exerc Metab. 2012 Dec;22(6):430-7. (PMID: 22805422) Appl Physiol Nutr Metab. 2013 Jul;38(7):760-5. (PMID: 23980734) |
فهرسة مساهمة: | Keywords: X-inactive specific transcript; microRNA-485; proteasome subunit β type-8; quercetin; rheumatoid arthritis |
تواريخ الأحداث: | Date Created: 20211004 Latest Revision: 20211005 |
رمز التحديث: | 20240628 |
مُعرف محوري في PubMed: | PMC8453329 |
DOI: | 10.3892/etm.2021.10695 |
PMID: | 34603528 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1792-1015 |
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DOI: | 10.3892/etm.2021.10695 |