دورية أكاديمية
Quercetin suppresses inflammatory cytokine production in rheumatoid arthritis fibroblast-like synoviocytes.
| العنوان: | Quercetin suppresses inflammatory cytokine production in rheumatoid arthritis fibroblast-like synoviocytes. |
|---|---|
| المؤلفون: | Sun HT; Laboratory of New Techniques of Restoration and Reconstruction of Orthopedics and Traumatology, Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210023, P.R. China.; Department of Emergency Trauma Center, Wuxi Huishan District People's Hospital, Wuxi, Jiangsu 214187, P.R. China., Li JP; Department of Orthopedics, Tianjin Medical University, Tianjin 300070, P.R. China.; Department of Orthopedics, Tianjin Fifth Central Hospital, Tianjin 300450, P.R. China., Qian WQ; Department of Orthopedics, The Third Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210001, P.R. China., Yin MF; Department of Orthopedics, Tianjin Fifth Central Hospital, Tianjin 300450, P.R. China., Yin H; Department of Orthopedics, The Third Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210001, P.R. China., Huang GC; Laboratory of New Techniques of Restoration and Reconstruction of Orthopedics and Traumatology, The First Clinical Medical College, Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210023, P.R. China. |
| المصدر: | Experimental and therapeutic medicine [Exp Ther Med] 2021 Nov; Vol. 22 (5), pp. 1260. Date of Electronic Publication: 2021 Sep 06. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Spandidos Pub.] Country of Publication: Greece NLM ID: 101531947 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1792-1015 (Electronic) Linking ISSN: 17920981 NLM ISO Abbreviation: Exp Ther Med Subsets: PubMed not MEDLINE |
| أسماء مطبوعة: | Original Publication: [Athens : Spandidos Pub.] |
| مستخلص: | Rheumatoid arthritis (RA) is a chronic, progressive and systemic autoimmune disease mainly characterized by symmetric multijoint synovitis. Quercetin has anti-inflammatory, anti-oxidation and immune regulation activities, and therefore shows high medicinal value. The present study aimed to observe the effect of quercetin on fibroblast-like synoviocytes (FLSs) in RA. Rheumatoid arthritis fibroblast-like synoviocytes (RAFLSs) were pretreated with 50 nmol/l quercetin for 2 h and were then stimulated using TNF-α for 24 h for subsequent experiments. RAFLSs were transfected with short interfering (si)-X-inactive specific transcript (XIST), microRNA (miR)-485 mimic, miR-485 inhibitor or si-PSMB8 or combination. ELISA, PCR and western blotting was used to evaluate the effect of quercetin on RAFLSs treated with TNF-α. It was revealed that quercetin inhibited the production of inflammatory cytokines and the expression of XIST in RAFLSs induced by TNF-α. Bioinformatics analysis indicated that XIST acted as a sponge for miR-485 and that proteasome subunit β type-8 (PSMB8) was a direct target of miR-485. Moreover, PSMB8 functioned as a suppressor in inflammatory cytokine production of RAFLSs induced by TNF-α. Overall, quercetin was observed to inhibit the production of inflammatory cytokines and the expression of XIST in RAFLSs induced by TNF-α. Moreover, XIST-silencing could suppress the inflammatory reaction by sponging miR-485 in cells treated with TNF-α. Altogether, quercetin could suppress the development of RA in vitro. Competing Interests: The authors declare that they have no competing interests. (Copyright: © Sun et al.) |
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| فهرسة مساهمة: | Keywords: X-inactive specific transcript; microRNA-485; proteasome subunit β type-8; quercetin; rheumatoid arthritis |
| تواريخ الأحداث: | Date Created: 20211004 Latest Revision: 20211005 |
| رمز التحديث: | 20240628 |
| مُعرف محوري في PubMed: | PMC8453329 |
| DOI: | 10.3892/etm.2021.10695 |
| PMID: | 34603528 |
| قاعدة البيانات: | MEDLINE |
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