دورية أكاديمية
Metabolic Labeling of Primary Neurons Using Carbohydrate Click Chemistry.
| العنوان: | Metabolic Labeling of Primary Neurons Using Carbohydrate Click Chemistry. |
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| المؤلفون: | Hayes JM; School of Biochemistry and Immunology, Trinity Biomedical Sciences Institute, Trinity College Dublin, Dublin, Ireland., O'Hara DM; School of Biochemistry and Immunology, Trinity Biomedical Sciences Institute, Trinity College Dublin, Dublin, Ireland., Davey GP; School of Biochemistry and Immunology, Trinity College Dublin, Dublin, Ireland. gdavey@tcd.ie. |
| المصدر: | Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2022; Vol. 2370, pp. 315-322. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Totowa, NJ : Humana Press Original Publication: Clifton, N.J. : Humana Press, |
| مواضيع طبية MeSH: | Carbohydrates* , Click Chemistry* , Neurons*/chemistry, Animals ; Glycosylation ; Polysaccharides ; Rats |
| مستخلص: | Glycans play an important role in many neuronal processes, such as neurotransmitter release and reuptake, cell-cell communication and adhesion, modulation of ion channel activity, and immune function. Carbohydrate click chemistry is a powerful technique for studying glycan function and dynamics in vitro, in vivo, and ex vivo. Here, we use commercially available synthetic tetraacetylated azido sugars, copper and copper-free click chemistry to metabolically label and analyze primary rat cortical neurons. In addition, we use high resolution confocal and STED microscopy to image and analyze different forms of glycosylation in ultrahigh resolution. We observe different patterns of GlcNAz, GalNAz, and ManNAz distribution at different stages of neuronal development. We also observe highly sialylated structures on the neuronal plasma membrane, which warrant further investigation. (© 2022. Springer Science+Business Media, LLC, part of Springer Nature.) |
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| فهرسة مساهمة: | Keywords: Click chemistry; Confocal microscopy; Glycosylation; Neurons; STED imaging; Sialylation |
| المشرفين على المادة: | 0 (Carbohydrates) 0 (Polysaccharides) |
| تواريخ الأحداث: | Date Created: 20211006 Date Completed: 20211220 Latest Revision: 20211220 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1007/978-1-0716-1685-7_16 |
| PMID: | 34611877 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1940-6029 |
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| DOI: | 10.1007/978-1-0716-1685-7_16 |