دورية أكاديمية
Micropattern platform promotes extracellular matrix remodeling by human PSC-derived cardiac fibroblasts and enhances contractility of co-cultured cardiomyocytes.
العنوان: | Micropattern platform promotes extracellular matrix remodeling by human PSC-derived cardiac fibroblasts and enhances contractility of co-cultured cardiomyocytes. |
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المؤلفون: | Napiwocki BN; Department of Biomedical Engineering, University of Wisconsin-Madison, Madison, Wisconsin, USA.; Wisconsin Institute for Discovery, University of Wisconsin-Madison, Madison, Wisconsin, USA., Stempien A; Department of Biomedical Engineering, University of Wisconsin-Madison, Madison, Wisconsin, USA.; Wisconsin Institute for Discovery, University of Wisconsin-Madison, Madison, Wisconsin, USA., Lang D; Department of Medicine, Division of Cardiovascular Medicine, University of Wisconsin-Madison, Madison, Wisconsin, USA., Kruepke RA; Engineering Mechanics Program, University of Wisconsin-Madison, Madison, Wisconsin, USA., Kim G; Department of Medicine, Division of Cardiovascular Medicine, University of Wisconsin-Madison, Madison, Wisconsin, USA., Zhang J; Department of Medicine, Division of Cardiovascular Medicine, University of Wisconsin-Madison, Madison, Wisconsin, USA., Eckhardt LL; Department of Medicine, Division of Cardiovascular Medicine, University of Wisconsin-Madison, Madison, Wisconsin, USA., Glukhov AV; Department of Medicine, Division of Cardiovascular Medicine, University of Wisconsin-Madison, Madison, Wisconsin, USA., Kamp TJ; Department of Medicine, Division of Cardiovascular Medicine, University of Wisconsin-Madison, Madison, Wisconsin, USA.; Department of Cell and Regenerative Biology, University of Wisconsin-Madison, Madison, Wisconsin, USA., Crone WC; Department of Biomedical Engineering, University of Wisconsin-Madison, Madison, Wisconsin, USA.; Wisconsin Institute for Discovery, University of Wisconsin-Madison, Madison, Wisconsin, USA.; Engineering Mechanics Program, University of Wisconsin-Madison, Madison, Wisconsin, USA.; Department of Engineering Physics, University of Wisconsin-Madison, Madison, Wisconsin, USA. |
المصدر: | Physiological reports [Physiol Rep] 2021 Oct; Vol. 9 (19), pp. e15045. |
نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
اللغة: | English |
بيانات الدورية: | Publisher: published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society Country of Publication: United States NLM ID: 101607800 Publication Model: Print Cited Medium: Internet ISSN: 2051-817X (Electronic) Linking ISSN: 2051817X NLM ISO Abbreviation: Physiol Rep Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: [Malden MA] : published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society, 2013- |
مواضيع طبية MeSH: | Extracellular Matrix/*metabolism , Fibroblasts/*metabolism , Myocytes, Cardiac/*metabolism, Cell Differentiation/physiology ; Coculture Techniques ; Fibroblasts/cytology ; Humans ; Induced Pluripotent Stem Cells/cytology ; Induced Pluripotent Stem Cells/metabolism ; Myocytes, Cardiac/cytology |
مستخلص: | In native heart tissue, cardiac fibroblasts provide the structural framework of extracellular matrix (ECM) while also influencing the electrical and mechanical properties of cardiomyocytes. Recent advances in the field of stem cell differentiation have led to the availability of human pluripotent stem cell-derived cardiac fibroblasts (iPSC-CFs) in addition to cardiomyocytes (iPSC-CMs). Here we use a novel 2D in vitro micropatterned platform that provides control over ECM geometry and substrate stiffness. When cultured alone on soft micropatterned substrates, iPSC-CFs are confined to the micropatterned features and remodel the ECM into anisotropic fibers. Similar remodeling and ECM production occurs when cultured with iPSC-CMs in a co-culture model. In addition to modifications in the ECM, our results show that iPSC-CFs influence iPSC-CM function with accelerated Ca 2+ transient rise-up time and greater contractile strains in the co-culture conditions compared to when iPSC-CMs are cultured alone. These combined observations highlight the important role cardiac fibroblasts play in vivo and the need for co-culture models like the one presented here to provide more representative in vitro cardiac constructs. (© 2021 The Authors. Physiological Reports published by Wiley Periodicals LLC on behalf of The Physiological Society and the American Physiological Society.) |
References: | Front Physiol. 2012 Sep 05;3:345. (PMID: 22973235) J Mol Cell Cardiol. 2016 May;94:22-31. (PMID: 26996756) Int J Cell Biol. 2012;2012:508294. (PMID: 22649451) Nat Protoc. 2014 Feb;9(2):457-63. (PMID: 24481272) Biomaterials. 2009 Oct;30(29):5433-44. (PMID: 19595452) Am J Physiol Heart Circ Physiol. 2006 Sep;291(3):H1015-26. (PMID: 16617141) Exp Mech. 2019 Nov;59(9):1235-1248. (PMID: 31680699) Bioinformatics. 2016 Mar 15;32(6):955-7. (PMID: 26589273) J Mol Cell Cardiol. 2014 May;70:47-55. (PMID: 24625635) Nat Commun. 2017 Nov 28;8(1):1825. (PMID: 29184059) Biotechnol Bioeng. 2021 Jan;118(1):442-452. (PMID: 32990953) Pharmacol Ther. 2009 Aug;123(2):255-78. (PMID: 19460403) Stem Cells Dev. 2010 Jun;19(6):783-95. (PMID: 20001453) J Clin Invest. 1994 Jun;93(6):2372-8. (PMID: 8200970) Biosens Bioelectron. 2020 Nov 1;167:112468. (PMID: 32829174) Cell Stem Cell. 2013 Jan 3;12(1):127-37. (PMID: 23168164) Biomaterials. 2013 Jul;34(23):5813-20. (PMID: 23642535) Circulation. 2006 Jul 4;114(1 Suppl):I72-8. (PMID: 16820649) Circulation. 2011 May 17;123(19):2083-93. (PMID: 21537003) Tissue Eng Part C Methods. 2011 Apr;17(4):463-73. (PMID: 21142417) Proc Natl Acad Sci U S A. 2014 Feb 4;111(5):1807-12. (PMID: 24449852) Nat Commun. 2016 Jan 19;7:10312. (PMID: 26785135) Proc Natl Acad Sci U S A. 2015 Oct 13;112(41):12705-10. (PMID: 26417073) Sci Rep. 2019 Jun 20;9(1):8879. (PMID: 31222006) Am J Physiol Cell Physiol. 2000 Jan;278(1):C154-62. (PMID: 10644523) Nat Commun. 2019 May 20;10(1):2238. (PMID: 31110246) Circ Res. 2012 Jun 8;110(12):1556-63. (PMID: 22570367) Cell. 2019 Feb 7;176(4):913-927.e18. (PMID: 30686581) Biophys J. 2009 May 6;96(9):3873-85. (PMID: 19413993) Sci Rep. 2017 Feb 09;7:42290. (PMID: 28181589) Circulation. 2017 May 9;135(19):1832-1847. (PMID: 28167635) Circ Arrhythm Electrophysiol. 2015 Apr;8(2):409-19. (PMID: 25673629) Heart Rhythm. 2009 Nov;6(11):1641-9. (PMID: 19879544) Nat Methods. 2013 Aug;10(8):781-7. (PMID: 23793239) Stem Cell Res. 2013 Nov;11(3):1335-47. (PMID: 24095945) Nature. 2018 Apr;556(7700):239-243. (PMID: 29618819) Circ Res. 2009 Feb 27;104(4):e30-41. (PMID: 19213953) Dev Biol. 2012 Jun 15;366(2):111-24. (PMID: 22546693) J Biomed Mater Res A. 2008 Sep;86(3):713-24. (PMID: 18041719) Acta Biomater. 2012 Mar;8(3):1267-72. (PMID: 21945825) Biomaterials. 2014 May;35(15):4454-64. (PMID: 24582552) Adv Funct Mater. 2014 May 28;24(20):3027-3035. (PMID: 25484849) Circ Res. 2006 Mar 31;98(6):801-10. (PMID: 16484613) Nat Protoc. 2013 Jan;8(1):162-75. (PMID: 23257984) PLoS One. 2012;7(12):e51499. (PMID: 23240031) J Clin Invest. 2014 Jul;124(7):2921-34. (PMID: 24937432) J Acquir Immune Defic Syndr Hum Retrovirol. 1995 Nov 1;10(3):295-305. (PMID: 7552491) Development. 2016 Feb 1;143(3):387-97. (PMID: 26839342) Proc Natl Acad Sci U S A. 2010 Jan 12;107(2):565-70. (PMID: 20018748) Circ Res. 2009 Dec 4;105(12):1164-76. (PMID: 19959782) Circulation. 2016 Nov 15;134(20):1557-1567. (PMID: 27737958) Physiol Rep. 2021 Oct;9(19):e15045. (PMID: 34617673) Biotechnol Bioeng. 2019 Sep;116(9):2377-2392. (PMID: 31131875) Cardiovasc Res. 2000 Jan 1;45(1):231-7. (PMID: 10728340) Differentiation. 2012 Jul;84(1):41-53. (PMID: 22652098) J Clin Invest. 1998 Jan 15;101(2):301-10. (PMID: 9435301) Circ Res. 1979 May;44(5):701-12. (PMID: 428066) |
معلومات مُعتمدة: | T32 HL007936 United States HL NHLBI NIH HHS; R01 HL128598 United States HL NHLBI NIH HHS; L30 HL115783 United States HL NHLBI NIH HHS; R01 HL139738 United States HL NHLBI NIH HHS; U01 HL134764 United States HL NHLBI NIH HHS |
فهرسة مساهمة: | Keywords: cardiac fibroblast; cardiomyocyte; extracellular matrix; human pluripotent stem cell; micropattern |
تواريخ الأحداث: | Date Created: 20211007 Date Completed: 20220309 Latest Revision: 20240403 |
رمز التحديث: | 20240403 |
مُعرف محوري في PubMed: | PMC8496154 |
DOI: | 10.14814/phy2.15045 |
PMID: | 34617673 |
قاعدة البيانات: | MEDLINE |
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