دورية أكاديمية
Micropattern platform promotes extracellular matrix remodeling by human PSC-derived cardiac fibroblasts and enhances contractility of co-cultured cardiomyocytes.
| العنوان: | Micropattern platform promotes extracellular matrix remodeling by human PSC-derived cardiac fibroblasts and enhances contractility of co-cultured cardiomyocytes. |
|---|---|
| المؤلفون: | Napiwocki BN; Department of Biomedical Engineering, University of Wisconsin-Madison, Madison, Wisconsin, USA.; Wisconsin Institute for Discovery, University of Wisconsin-Madison, Madison, Wisconsin, USA., Stempien A; Department of Biomedical Engineering, University of Wisconsin-Madison, Madison, Wisconsin, USA.; Wisconsin Institute for Discovery, University of Wisconsin-Madison, Madison, Wisconsin, USA., Lang D; Department of Medicine, Division of Cardiovascular Medicine, University of Wisconsin-Madison, Madison, Wisconsin, USA., Kruepke RA; Engineering Mechanics Program, University of Wisconsin-Madison, Madison, Wisconsin, USA., Kim G; Department of Medicine, Division of Cardiovascular Medicine, University of Wisconsin-Madison, Madison, Wisconsin, USA., Zhang J; Department of Medicine, Division of Cardiovascular Medicine, University of Wisconsin-Madison, Madison, Wisconsin, USA., Eckhardt LL; Department of Medicine, Division of Cardiovascular Medicine, University of Wisconsin-Madison, Madison, Wisconsin, USA., Glukhov AV; Department of Medicine, Division of Cardiovascular Medicine, University of Wisconsin-Madison, Madison, Wisconsin, USA., Kamp TJ; Department of Medicine, Division of Cardiovascular Medicine, University of Wisconsin-Madison, Madison, Wisconsin, USA.; Department of Cell and Regenerative Biology, University of Wisconsin-Madison, Madison, Wisconsin, USA., Crone WC; Department of Biomedical Engineering, University of Wisconsin-Madison, Madison, Wisconsin, USA.; Wisconsin Institute for Discovery, University of Wisconsin-Madison, Madison, Wisconsin, USA.; Engineering Mechanics Program, University of Wisconsin-Madison, Madison, Wisconsin, USA.; Department of Engineering Physics, University of Wisconsin-Madison, Madison, Wisconsin, USA. |
| المصدر: | Physiological reports [Physiol Rep] 2021 Oct; Vol. 9 (19), pp. e15045. |
| نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society Country of Publication: United States NLM ID: 101607800 Publication Model: Print Cited Medium: Internet ISSN: 2051-817X (Electronic) Linking ISSN: 2051817X NLM ISO Abbreviation: Physiol Rep Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: [Malden MA] : published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society, 2013- |
| مواضيع طبية MeSH: | Extracellular Matrix/*metabolism , Fibroblasts/*metabolism , Myocytes, Cardiac/*metabolism, Cell Differentiation/physiology ; Coculture Techniques ; Fibroblasts/cytology ; Humans ; Induced Pluripotent Stem Cells/cytology ; Induced Pluripotent Stem Cells/metabolism ; Myocytes, Cardiac/cytology |
| مستخلص: | In native heart tissue, cardiac fibroblasts provide the structural framework of extracellular matrix (ECM) while also influencing the electrical and mechanical properties of cardiomyocytes. Recent advances in the field of stem cell differentiation have led to the availability of human pluripotent stem cell-derived cardiac fibroblasts (iPSC-CFs) in addition to cardiomyocytes (iPSC-CMs). Here we use a novel 2D in vitro micropatterned platform that provides control over ECM geometry and substrate stiffness. When cultured alone on soft micropatterned substrates, iPSC-CFs are confined to the micropatterned features and remodel the ECM into anisotropic fibers. Similar remodeling and ECM production occurs when cultured with iPSC-CMs in a co-culture model. In addition to modifications in the ECM, our results show that iPSC-CFs influence iPSC-CM function with accelerated Ca 2+ transient rise-up time and greater contractile strains in the co-culture conditions compared to when iPSC-CMs are cultured alone. These combined observations highlight the important role cardiac fibroblasts play in vivo and the need for co-culture models like the one presented here to provide more representative in vitro cardiac constructs. (© 2021 The Authors. Physiological Reports published by Wiley Periodicals LLC on behalf of The Physiological Society and the American Physiological Society.) |
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| معلومات مُعتمدة: | T32 HL007936 United States HL NHLBI NIH HHS; R01 HL128598 United States HL NHLBI NIH HHS; L30 HL115783 United States HL NHLBI NIH HHS; R01 HL139738 United States HL NHLBI NIH HHS; U01 HL134764 United States HL NHLBI NIH HHS |
| فهرسة مساهمة: | Keywords: cardiac fibroblast; cardiomyocyte; extracellular matrix; human pluripotent stem cell; micropattern |
| تواريخ الأحداث: | Date Created: 20211007 Date Completed: 20220309 Latest Revision: 20240403 |
| رمز التحديث: | 20240403 |
| مُعرف محوري في PubMed: | PMC8496154 |
| DOI: | 10.14814/phy2.15045 |
| PMID: | 34617673 |
| قاعدة البيانات: | MEDLINE |
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