دورية أكاديمية
أعرض في EDS

Role of miR-2392 in driving SARS-CoV-2 infection.

التفاصيل البيبلوغرافية
العنوان: Role of miR-2392 in driving SARS-CoV-2 infection.
المؤلفون: McDonald JT; COVID-19 International Research Team; Georgetown University School of Medicine, Washington, DC 20007, USA., Enguita FJ; COVID-19 International Research Team; Instituto de Medicina Molecular João Lobo Antunes, Universidade de Lisboa, Av. Prof. Egas Moniz, 1649-028 Lisbon, Portugal., Taylor D; COVID-19 International Research Team; The Children's Hospital of Philadelphia, Philadelphia, PA 19104, USA; University of Pennsylvania, Philadelphia, PA 19104, USA., Griffin RJ; COVID-19 International Research Team; University of Arkansas for Medical Sciences, Little Rock, AK 72211, USA., Priebe W; COVID-19 International Research Team; University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA., Emmett MR; COVID-19 International Research Team; University of Texas Medical Branch, Galveston, TX 77555, USA., Sajadi MM; University of Maryland School of Medicine, Baltimore, MD 21201, USA., Harris AD; University of Maryland School of Medicine, Baltimore, MD 21201, USA., Clement J; University of Maryland School of Medicine, Baltimore, MD 21201, USA., Dybas JM; COVID-19 International Research Team; The Children's Hospital of Philadelphia, Philadelphia, PA 19104, USA., Aykin-Burns N; University of Pennsylvania, Philadelphia, PA 19104, USA., Guarnieri JW; COVID-19 International Research Team; The Children's Hospital of Philadelphia, Philadelphia, PA 19104, USA., Singh LN; COVID-19 International Research Team; The Children's Hospital of Philadelphia, Philadelphia, PA 19104, USA., Grabham P; COVID-19 International Research Team; Columbia University, New York, NY 10032, USA., Baylin SB; COVID-19 International Research Team; Johns Hopkins School of Medicine, Baltimore, MD 21287, USA., Yousey A; COVID-19 International Research Team; Morehouse School of Medicine, Atlanta, GA 30310, USA., Pearson AN; Morehouse School of Medicine, Atlanta, GA 30310, USA., Corry PM; COVID-19 International Research Team; University of Arkansas for Medical Sciences, Little Rock, AK 72211, USA., Saravia-Butler A; COVID-19 International Research Team; Logyx LLC, Mountain View, CA 94043, USA; NASA Ames Research Center, Moffett Field, CA 94035, USA., Aunins TR; University of Colorado Boulder, Boulder, CO 80303, USA., Sharma S; University of Colorado Boulder, Boulder, CO 80303, USA; Sachi Bioworks Inc., Boulder, CO 80301, USA., Nagpal P; Sachi Bioworks Inc., Boulder, CO 80301, USA; Antimicrobial Regeneration Consortium, Boulder Labs, Boulder, CO 80301, USA; Quantum Biology Inc., Boulder, CO 80301, USA., Meydan C; Weill Cornell Medicine, New York, NY 10065, USA., Foox J; Weill Cornell Medicine, New York, NY 10065, USA., Mozsary C; Weill Cornell Medicine, New York, NY 10065, USA., Cerqueira B; COVID-19 International Research Team; KBR Space & Science, San Antonio, TX 78235, USA; United States Air Force School of Aerospace Medicine, Lackland AFB, San Antonio, TX 78236, USA., Zaksas V; COVID-19 International Research Team; University of Chicago, Chicago, IL 60615, USA., Singh U; COVID-19 International Research Team; Iowa State University, Ames, IA 50011, USA., Wurtele ES; COVID-19 International Research Team; Iowa State University, Ames, IA 50011, USA., Costes SV; NASA Ames Research Center, Moffett Field, CA 94035, USA., Davanzo GG; University of Campinas, Sao Paulo, Brazil., Galeano D; COVID-19 International Research Team; Fundação Getulio Vargas, Rio de Janeiro, Brazil; National University of Asuncion, San Lorenzo, Central, Paraguay., Paccanaro A; COVID-19 International Research Team; Fundação Getulio Vargas, Rio de Janeiro, Brazil; University of London, Egham Hill, Egham, UK., Meinig SL; University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA., Hagan RS; University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA., Bowman NM; University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA., Wolfgang MC; University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA., Altinok S; University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA., Sapoval N; Rice University, Houston, TX 77005, USA., Treangen TJ; Rice University, Houston, TX 77005, USA., Moraes-Vieira PM; COVID-19 International Research Team; University of Campinas, Sao Paulo, Brazil., Vanderburg C; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA., Wallace DC; COVID-19 International Research Team; The Children's Hospital of Philadelphia, Philadelphia, PA 19104, USA; University of Pennsylvania, Philadelphia, PA 19104, USA., Schisler JC; COVID-19 International Research Team; University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA., Mason CE; COVID-19 International Research Team; Weill Cornell Medicine, New York, NY 10065, USA; New York Genome Center, New York, NY, USA., Chatterjee A; COVID-19 International Research Team; University of Colorado Boulder, Boulder, CO 80303, USA; Sachi Bioworks Inc., Boulder, CO 80301, USA; Antimicrobial Regeneration Consortium, Boulder Labs, Boulder, CO 80301, USA., Meller R; COVID-19 International Research Team; Morehouse School of Medicine, Atlanta, GA 30310, USA., Beheshti A; COVID-19 International Research Team; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA; KBR, NASA Ames Research Center, Moffett Field, CA 94035, USA. Electronic address: afshin.beheshti@nasa.gov.
مؤلفون مشاركون: UNC COVID-19 Pathobiology Consortium; University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA.
المصدر: Cell reports [Cell Rep] 2021 Oct 19; Vol. 37 (3), pp. 109839. Date of Electronic Publication: 2021 Sep 30.
نوع المنشور: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.
اللغة: English
بيانات الدورية: Publisher: Cell Press Country of Publication: United States NLM ID: 101573691 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2211-1247 (Electronic) NLM ISO Abbreviation: Cell Rep Subsets: MEDLINE
أسماء مطبوعة: Original Publication: [Cambridge, MA] : Cell Press, c 2012-
مواضيع طبية MeSH: COVID-19/*genetics , COVID-19/*immunology , MicroRNAs/*genetics , SARS-CoV-2/*genetics, Adult ; Aged ; Aged, 80 and over ; Animals ; Antiviral Agents/pharmacology ; Biomarkers/metabolism ; Cricetinae ; Female ; Ferrets ; Gene Expression Regulation ; Glycolysis ; Healthy Volunteers ; Humans ; Hypoxia ; Inflammation ; Male ; Mice ; Middle Aged ; Proteomics/methods ; ROC Curve ; Rats ; COVID-19 Drug Treatment
مستخلص: MicroRNAs (miRNAs) are small non-coding RNAs involved in post-transcriptional gene regulation that have a major impact on many diseases and provide an exciting avenue toward antiviral therapeutics. From patient transcriptomic data, we determined that a circulating miRNA, miR-2392, is directly involved with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) machinery during host infection. Specifically, we show that miR-2392 is key in driving downstream suppression of mitochondrial gene expression, increasing inflammation, glycolysis, and hypoxia, as well as promoting many symptoms associated with coronavirus disease 2019 (COVID-19) infection. We demonstrate that miR-2392 is present in the blood and urine of patients positive for COVID-19 but is not present in patients negative for COVID-19. These findings indicate the potential for developing a minimally invasive COVID-19 detection method. Lastly, using in vitro human and in vivo hamster models, we design a miRNA-based antiviral therapeutic that targets miR-2392, significantly reduces SARS-CoV-2 viability in hamsters, and may potentially inhibit a COVID-19 disease state in humans.
Competing Interests: Declaration of interests A.C., P.N., S.V.C., and A.B. have a provisional patent based on the antiviral discovery and design. A.C., P.N., and S.S. are part of the company (Sachi Bioworks Inc.) that has filed a patent on the Nanoligomer technology.
(Copyright © 2021 The Author(s). Published by Elsevier Inc. All rights reserved.)
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معلومات مُعتمدة: BB/F00964X/1 United Kingdom BB_ Biotechnology and Biological Sciences Research Council; R03 HL155249 United States HL NHLBI NIH HHS; UL1 TR002489 United States TR NCATS NIH HHS; U54 CA260543 United States CA NCI NIH HHS; K23 DK059311 United States DK NIDDK NIH HHS; F32 AI147587 United States AI NIAID NIH HHS; R01 NS104349 United States NS NINDS NIH HHS; P20 GM109005 United States GM NIGMS NIH HHS; R01 NS112422 United States NS NINDS NIH HHS; K08 HL143271 United States HL NHLBI NIH HHS; R01 AI147870 United States AI NIAID NIH HHS
فهرسة مساهمة: Investigator: SM Wallet; R Maile; MC Wolfgang; RS Hagan; JR Mock; NM Bowman; JL Torres-Castillo; MK Love; SL Meinig; W Lovell; C Rice; O Mitchem; D Burgess; J Suggs; J Jacobs
Keywords: COVID-19; SARS-CoV-2; antiviral therapeutic; biomarker; miR-2392; miRNA; microRNA; nanoligomers
المشرفين على المادة: 0 (Antiviral Agents)
0 (Biomarkers)
0 (MIRN2392 microRNA, human)
0 (MicroRNAs)
تواريخ الأحداث: Date Created: 20211008 Date Completed: 20211115 Latest Revision: 20240703
رمز التحديث: 20240703
مُعرف محوري في PubMed: PMC8481092
DOI: 10.1016/j.celrep.2021.109839
PMID: 34624208
قاعدة البيانات: MEDLINE
الوصف
تدمد:2211-1247
DOI:10.1016/j.celrep.2021.109839