دورية أكاديمية
أعرض في EDS

Hofmeister Effect in RT-QuIC Seeding Activity of Chronic Wasting Disease Prions.

التفاصيل البيبلوغرافية
العنوان: Hofmeister Effect in RT-QuIC Seeding Activity of Chronic Wasting Disease Prions.
المؤلفون: Hwang S; United States Department of Agriculture, Agricultural Research Service, National Animal Disease Center, Virus and Prion Research Unit, Ames, IA, United States., Beckley D; United States Department of Agriculture, Agricultural Research Service, National Animal Disease Center, Virus and Prion Research Unit, Ames, IA, United States.; U.S. Department of Energy, Oak Ridge Institute for Science and Education, Oak Ridge, TN, United States., Alekseev KP; United States Department of Agriculture, Agricultural Research Service, National Animal Disease Center, Virus and Prion Research Unit, Ames, IA, United States.; U.S. Department of Energy, Oak Ridge Institute for Science and Education, Oak Ridge, TN, United States.; N. F. Gamaleya National Research Center for Epidemiology and Microbiology, Moscow, Russia., Nicholson EM; United States Department of Agriculture, Agricultural Research Service, National Animal Disease Center, Virus and Prion Research Unit, Ames, IA, United States.
المصدر: Frontiers in bioengineering and biotechnology [Front Bioeng Biotechnol] 2021 Sep 30; Vol. 9, pp. 709965. Date of Electronic Publication: 2021 Sep 30 (Print Publication: 2021).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Frontiers Media S.A Country of Publication: Switzerland NLM ID: 101632513 Publication Model: eCollection Cited Medium: Print ISSN: 2296-4185 (Print) Linking ISSN: 22964185 NLM ISO Abbreviation: Front Bioeng Biotechnol Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: Lausanne : Frontiers Media S.A., [2013]-
مستخلص: Chronic wasting disease (CWD) is a transmissible spongiform encephalopathy (TSE) that causes a fatal neurodegenerative disease in cervids. Cases of CWD are rapidly increasing in North America among wild and farmed cervid populations, and potential for zoonotic transmission is not yet determined. Therefore, in order to manage the disease, it is imperative to devise a system that can detect CWD during its early phases to prevent spread to new captive herds through introduction of CWD-affected animals into otherwise CWD-free herds. Real-time quaking-induced conversion (RT-QuIC) assays have been applied to detect the presence of disease-associated prions from various samples in both animals and humans. In this study, we have tested the use of five Hofmeister anions that range from weakly hydrating to strongly hydrating: Na 3 citrate, Na 2 SO 4 , NaCl, NaI, and NaClO 4 in RT-QuIC reactions for CWD seeding activity using different recombinant prion proteins as substrates. This work shows how the ionic environment of the RT-QuIC reaction can enhance or diminish the seeding activity. The use of Na 2 SO 4 or NaI as the sodium salt for RT-QuIC using bank vole recombinant prion substrate for the detection of CWD using brain samples reduces the lag time to detect with reasonable specificity. For detection of the CWD in fecal samples, only NaI showed comparable reduction in lag time relative to NaCl but required reduced temperature to alleviate spontaneous fibril formation in negative control samples. Selection of the proper ion environment and recombinant prion protein substrate will make RT-QuIC a powerful diagnostic tool for early detection of CWD prions, further supporting CWD surveillance in wild and captive cervids.
Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
(Copyright © 2021 Hwang, Beckley, Alekseev and Nicholson.)
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فهرسة مساهمة: Keywords: Hofmeister series; RT-QuIC; chronic wasting disease (CWD); diagnostics; prion diseases; salt ions; seeding activity; transmissible spongiform encephalopathy
تواريخ الأحداث: Date Created: 20211018 Latest Revision: 20211022
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC8515057
DOI: 10.3389/fbioe.2021.709965
PMID: 34660549
قاعدة البيانات: MEDLINE
الوصف
تدمد:2296-4185
DOI:10.3389/fbioe.2021.709965