دورية أكاديمية
A natural protein based platform for the delivery of Temozolomide acid to glioma cells.
| العنوان: | A natural protein based platform for the delivery of Temozolomide acid to glioma cells. |
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| المؤلفون: | Helal DO; School of Cancer and Pharmaceutical Sciences, Faculty of Life Sciences & Medicine, King's College London, 150 Stamford Street, London SE1 9NH, United Kingdom; Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, Ain Shams University, Monazzamet El Wehda El Afrikeya Street, Abbasseya, Cairo, Egypt., Rouatbi N; School of Cancer and Pharmaceutical Sciences, Faculty of Life Sciences & Medicine, King's College London, 150 Stamford Street, London SE1 9NH, United Kingdom., Han S; School of Cancer and Pharmaceutical Sciences, Faculty of Life Sciences & Medicine, King's College London, 150 Stamford Street, London SE1 9NH, United Kingdom., Tzu-Wen Wang J; School of Cancer and Pharmaceutical Sciences, Faculty of Life Sciences & Medicine, King's College London, 150 Stamford Street, London SE1 9NH, United Kingdom., Walters AA; School of Cancer and Pharmaceutical Sciences, Faculty of Life Sciences & Medicine, King's College London, 150 Stamford Street, London SE1 9NH, United Kingdom., Abdel-Mottaleb MMA; Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, Ain Shams University, Monazzamet El Wehda El Afrikeya Street, Abbasseya, Cairo, Egypt., Kamel AO; Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, Ain Shams University, Monazzamet El Wehda El Afrikeya Street, Abbasseya, Cairo, Egypt., Geneidi AS; Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, Ain Shams University, Monazzamet El Wehda El Afrikeya Street, Abbasseya, Cairo, Egypt., Awad GAS; Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, Ain Shams University, Monazzamet El Wehda El Afrikeya Street, Abbasseya, Cairo, Egypt., Al-Jamal KT; School of Cancer and Pharmaceutical Sciences, Faculty of Life Sciences & Medicine, King's College London, 150 Stamford Street, London SE1 9NH, United Kingdom. Electronic address: khuloud.al-jamal@kcl.ac.uk. |
| المصدر: | European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V [Eur J Pharm Biopharm] 2021 Dec; Vol. 169, pp. 297-308. Date of Electronic Publication: 2021 Oct 19. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Science Country of Publication: Netherlands NLM ID: 9109778 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-3441 (Electronic) Linking ISSN: 09396411 NLM ISO Abbreviation: Eur J Pharm Biopharm Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Amsterdam : Elsevier Science Original Publication: Stuttgart : Wissenschaftliche Verlagsgesellschaft, c1991- |
| مواضيع طبية MeSH: | Glioma*/drug therapy , Glioma*/metabolism , Glioma*/pathology , Temozolomide*/administration & dosage , Temozolomide*/pharmacokinetics, Nanoparticles/*therapeutic use , Osteonectin/*metabolism , Serum Albumin, Human/*pharmacology, Animals ; Antineoplastic Agents, Alkylating/administration & dosage ; Antineoplastic Agents, Alkylating/pharmacokinetics ; Biological Products/pharmacology ; Cell Line, Tumor ; Cell Survival/drug effects ; Drug Compounding/methods ; Drug Delivery Systems/methods ; Drug Liberation ; Drug Stability ; Mice ; Particle Size ; Tissue Distribution |
| مستخلص: | Glioblastoma is one of the most difficult to treat cancers with poor prognosis and survival of around one year from diagnosis. Effective treatments are desperately needed. This work aims to prepare temozolomide acid (TMZA) loaded albumin nanoparticles, for the first time, to target glioblastoma (GL261) and brain cancer stem cells (BL6). TMZA was loaded into human serum albumin nanoparticles (HSA NPs) using the desolvation method. A response surface 3-level factorial design was used to study the effect of different formulation parameters on the drug loading and particle size of NPs. The optimum conditions were found to be: 4 mg TMZA with 0.05% sodium cholate. This yielded NPs with particle size and drug loading of 111.7 nm and 5.5% respectively. The selected formula was found to have good shelf life and serum stability but with a relatively fast drug release pattern. The optimized NPs showed excellent cellular uptake with ∼ 50 and 100% of cells were positive for NP uptake after 24 h incubation with both GL261 and BL6 glioblastoma cell lines, respectively. The selected formula showed high cytotoxicity with ̴ 20% cell viability at 1 mM TMZA after 72 h incubation time. Finally, the fluorescently labelled NPs showed co-localization with the bioluminescent syngeneic BL6 intra-cranial tumour mouse model after intravenous administration. (Copyright © 2021. Published by Elsevier B.V.) |
| فهرسة مساهمة: | Keywords: Glioma; Human serum albumin; Nanoparticles; SPARC; Temozolomide acid |
| المشرفين على المادة: | 0 (Antineoplastic Agents, Alkylating) 0 (Biological Products) 0 (Osteonectin) YF1K15M17Y (Temozolomide) ZIF514RVZR (Serum Albumin, Human) |
| تواريخ الأحداث: | Date Created: 20211022 Date Completed: 20220311 Latest Revision: 20220311 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1016/j.ejpb.2021.10.007 |
| PMID: | 34678408 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1873-3441 |
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| DOI: | 10.1016/j.ejpb.2021.10.007 |