دورية أكاديمية
أعرض في EDS

Purification of full-length recombinant human huntingtin proteins with allelic series of polyglutamine lengths.

التفاصيل البيبلوغرافية
العنوان: Purification of full-length recombinant human huntingtin proteins with allelic series of polyglutamine lengths.
المؤلفون: Kim H; Department of Biological Sciences, KI for the BioCentury, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 34141, South Korea., Hyun KG; Molecular Neurogenetics Unit, Center for Genomic Medicine, Massachusetts General Hospital, Boston, MA 02114, USA.; Department of Neurology, Harvard Medical School, Boston, MA 02114, USA., Lloret A; Molecular Neurogenetics Unit, Center for Genomic Medicine, Massachusetts General Hospital, Boston, MA 02114, USA.; Department of Neurology, Harvard Medical School, Boston, MA 02114, USA., Seong IS; Molecular Neurogenetics Unit, Center for Genomic Medicine, Massachusetts General Hospital, Boston, MA 02114, USA.; Department of Neurology, Harvard Medical School, Boston, MA 02114, USA., Song JJ; Department of Biological Sciences, KI for the BioCentury, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 34141, South Korea.
المصدر: STAR protocols [STAR Protoc] 2021 Oct 16; Vol. 2 (4), pp. 100886. Date of Electronic Publication: 2021 Oct 16 (Print Publication: 2021).
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Cell Press Country of Publication: United States NLM ID: 101769501 Publication Model: eCollection Cited Medium: Internet ISSN: 2666-1667 (Electronic) Linking ISSN: 26661667 NLM ISO Abbreviation: STAR Protoc
أسماء مطبوعة: Original Publication: [Cambridge, MA] : Cell Press, [2020]-
مواضيع طبية MeSH: Huntingtin Protein/*isolation & purification , Peptides/*genetics , Recombinant Proteins/*isolation & purification, Animals ; Baculoviridae/genetics ; Cell Culture Techniques ; Chromatography, Affinity/methods ; Humans ; Huntingtin Protein/chemistry ; Huntingtin Protein/genetics ; Huntingtin Protein/metabolism ; Recombinant Proteins/chemistry ; Recombinant Proteins/genetics ; Recombinant Proteins/metabolism ; Sf9 Cells
مستخلص: Huntington's disease (HD) is an autosomal dominant neurodegenerative disorder caused by the polyglutamine (polyQ) expansion in huntingtin (HTT) protein. The challenge of obtaining full-length HTT proteins with high purity limits the understanding of the HTT protein function. Here, we provide a protocol to generate and purify full-length recombinant human HTT proteins with various polyQ lengths, which is key to investigate the biochemical function of HTT proteins and the molecular mechanism underlying HD pathology. For complete details on the use and execution of this protocol, please refer to Jung et al. (2020).
Competing Interests: J.S. is a co-founder and CTO of PCG Biotech.
(© 2021 The Author(s).)
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فهرسة مساهمة: Keywords: Cryo-EM; Protein Biochemistry; Protein expression and purification
المشرفين على المادة: 0 (HTT protein, human)
0 (Huntingtin Protein)
0 (Peptides)
0 (Recombinant Proteins)
26700-71-0 (polyglutamine)
تواريخ الأحداث: Date Created: 20211108 Date Completed: 20220329 Latest Revision: 20240403
رمز التحديث: 20240403
مُعرف محوري في PubMed: PMC8551504
DOI: 10.1016/j.xpro.2021.100886
PMID: 34746859
قاعدة البيانات: MEDLINE
الوصف
تدمد:2666-1667
DOI:10.1016/j.xpro.2021.100886