دورية أكاديمية
Deficiency of TET3 leads to a genome-wide DNA hypermethylation episignature in human whole blood.
| العنوان: | Deficiency of TET3 leads to a genome-wide DNA hypermethylation episignature in human whole blood. |
|---|---|
| المؤلفون: | Levy MA; Molecular Genetics Laboratory, Molecular Diagnostics Division, London Health Sciences Centre, London, ON, N6A5W9, Canada., Beck DB; National Human Genome Research Institute, Bethesda, MD, 20892, USA., Metcalfe K; Division of Evolution, Infection and Genomics, School of Biological Sciences, Faculty of Biology, Medicine and Health, University of Manchester, Manchester, M13 9PL, UK.; Manchester Centre for Genomic Medicine, St Mary's Hospital, Health Innovation Manchester, Manchester University NHS Foundation Trust, Manchester, M13 9WL, UK., Douzgou S; Division of Evolution, Infection and Genomics, School of Biological Sciences, Faculty of Biology, Medicine and Health, University of Manchester, Manchester, M13 9PL, UK.; Manchester Centre for Genomic Medicine, St Mary's Hospital, Health Innovation Manchester, Manchester University NHS Foundation Trust, Manchester, M13 9WL, UK., Sithambaram S; Manchester Centre for Genomic Medicine, St Mary's Hospital, Health Innovation Manchester, Manchester University NHS Foundation Trust, Manchester, M13 9WL, UK., Cottrell T; Manchester Centre for Genomic Medicine, St Mary's Hospital, Health Innovation Manchester, Manchester University NHS Foundation Trust, Manchester, M13 9WL, UK., Ansar M; Department of Biochemistry, Faculty of Biological Sciences, Quaid-I-Azam University, 45320, Islamabad, Pakistan., Kerkhof J; Molecular Genetics Laboratory, Molecular Diagnostics Division, London Health Sciences Centre, London, ON, N6A5W9, Canada., Mignot C; Assistance Publique-Hopitaux de Paris, Sorbonne Université, Departement de Génétique, Groupe Hospitalier Pitie-Salpetriere et Hopital Trousseau, Paris, 75651, France., Nougues MC; Department of Neuropediatrics, Armand Trousseau Hospital, Assistance Publique-Hopitaux de Paris, Paris, 75012, France., Keren B; Laboratoire de génétique, Hôpital Pïtié-Salpêtrière, Assistance Publique-Hopitaux de Paris, Paris, 75013, France., Moore HW; Greenwood Genetic Center, Greenwood, SC, 29646, USA., Oegema R; Department of Genetics, University Medical Center Utrecht, Utrecht University, Utrecht, The Netherlands., Giltay JC; Department of Genetics, University Medical Center Utrecht, Utrecht University, Utrecht, The Netherlands., Simon M; Department of Genetics, University Medical Center Utrecht, Utrecht University, Utrecht, The Netherlands., van Jaarsveld RH; Department of Genetics, University Medical Center Utrecht, Utrecht University, Utrecht, The Netherlands., Bos J; Section Clinical Genetics, Department Human Genetics, Amsterdam University Medical Centers, Amsterdam, The Netherlands., van Haelst M; Section Clinical Genetics, Department Human Genetics, Amsterdam University Medical Centers, Amsterdam, The Netherlands., Motazacker MM; Department of Human Genetics, Laboratory of Genome Diagnostics, Amsterdam UMC, University of Amsterdam, Meibergdreef 9, Amsterdam, Netherlands., Boon EMJ; Department of Human Genetics, VU University Medical Center Amsterdam, Amsterdam UMC, van der Boechorststraat 7, 1081 BT, Amsterdam, The Netherlands., Santen GWE; Department of Clinical Genetics, Leiden University Medical Center, Leiden, The Netherlands., Ruivenkamp CAL; Department of Clinical Genetics, Leiden University Medical Center, Leiden, The Netherlands., Alders M; Department of Human Genetics, Amsterdam Reproduction & Development Research Institute, Amsterdam UMC, University of Amsterdam, Meibergdreef 9, Amsterdam, The Netherlands., Luperchio TR; Department of Genetic Medicine, Johns Hopkins University School of Medicine, Baltimore, MD, 21205, USA., Boukas L; Department of Genetic Medicine, Johns Hopkins University School of Medicine, Baltimore, MD, 21205, USA.; Department of Biostatistics, Johns Hopkins University, Baltimore, MD, 21205, USA., Ramsey K; Center for Rare Childhood Disorders, Translational Genomics Research Institute, Phoenix, AZ, USA., Narayanan V; Center for Rare Childhood Disorders, Translational Genomics Research Institute, Phoenix, AZ, USA., Schaefer GB; University of Arkansas for Medical Sciences, Springdale, AR, 72762, USA., Bonasio R; Department of Cell and Developmental Biology, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA, 19104, USA.; Epigenetics Institute, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA, 19104, USA., Doheny KF; Department of Genetic Medicine, Johns Hopkins University School of Medicine, Baltimore, MD, 21205, USA.; Center for Inherited Disease Research, Johns Hopkins University School of Medicine, Baltimore, MD, USA., Stevenson RE; Greenwood Genetic Center, Greenwood, SC, 29646, USA., Banka S; Division of Evolution, Infection and Genomics, School of Biological Sciences, Faculty of Biology, Medicine and Health, University of Manchester, Manchester, M13 9PL, UK.; Manchester Centre for Genomic Medicine, St Mary's Hospital, Health Innovation Manchester, Manchester University NHS Foundation Trust, Manchester, M13 9WL, UK., Sadikovic B; Molecular Genetics Laboratory, Molecular Diagnostics Division, London Health Sciences Centre, London, ON, N6A5W9, Canada. Bekim.Sadikovic@lhsc.on.ca.; Department of Pathology and Laboratory Medicine, Western University, London, ON, N6A5W9, Canada. Bekim.Sadikovic@lhsc.on.ca., Fahrner JA; Department of Genetic Medicine, Johns Hopkins University School of Medicine, Baltimore, MD, 21205, USA. jfahrne1@jhmi.edu.; Department of Pediatrics, Johns Hopkins University School of Medicine, Baltimore, MD, 21205, USA. jfahrne1@jhmi.edu. |
| المصدر: | NPJ genomic medicine [NPJ Genom Med] 2021 Nov 08; Vol. 6 (1), pp. 92. Date of Electronic Publication: 2021 Nov 08. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Springer Nature in partnership with the Center of Excellence in Genomic Medicine Research at King Abdulaziz University Country of Publication: England NLM ID: 101685193 Publication Model: Electronic Cited Medium: Internet ISSN: 2056-7944 (Electronic) Linking ISSN: 20567944 NLM ISO Abbreviation: NPJ Genom Med Subsets: PubMed not MEDLINE |
| أسماء مطبوعة: | Publication: 2016- : [London] : Springer Nature in partnership with the Center of Excellence in Genomic Medicine Research at King Abdulaziz University Original Publication: [London] : Nature Publishing Group, published in partnership with Center of Excellence in Genomic Medicine Research, [2016]- |
| مستخلص: | TET3 encodes an essential dioxygenase involved in epigenetic regulation through DNA demethylation. TET3 deficiency, or Beck-Fahrner syndrome (BEFAHRS; MIM: 618798), is a recently described neurodevelopmental disorder of the DNA demethylation machinery with a nonspecific phenotype resembling other chromatin-modifying disorders, but inconsistent variant types and inheritance patterns pose diagnostic challenges. Given TET3's direct role in regulating 5-methylcytosine and recent identification of syndrome-specific DNA methylation profiles, we analyzed genome-wide DNA methylation in whole blood of TET3-deficient individuals and identified an episignature that distinguishes affected and unaffected individuals and those with mono-allelic and bi-allelic pathogenic variants. Validation and testing of the episignature correctly categorized known TET3 variants and determined pathogenicity of variants of uncertain significance. Clinical utility was demonstrated when the episignature alone identified an affected individual from over 1000 undiagnosed cases and was confirmed upon distinguishing TET3-deficient individuals from those with 46 other disorders. The TET3-deficient signature - and the signature resulting from activating mutations in DNMT1 which normally opposes TET3 - are characterized by hypermethylation, which for BEFAHRS involves CpG sites that may be biologically relevant. This work expands the role of epi-phenotyping in molecular diagnosis and reveals genome-wide DNA methylation profiling as a quantitative, functional readout for characterization of this new biochemical category of disease. (© 2021. The Author(s).) |
| التعليقات: | Erratum in: NPJ Genom Med. 2021 Nov 24;6(1):100. (PMID: 34819528) |
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| معلومات مُعتمدة: | P50HD103538 U.S. Department of Health & Human Services | NIH | Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD); P50 HD103538 United States HD NICHD NIH HHS; IBRA Hartwell Foundation (The Hartwell Foundation); K08 HD086250 United States HD NICHD NIH HHS; United Kingdom WT_ Wellcome Trust; K08HD086250 U.S. Department of Health & Human Services | NIH | Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD) |
| تواريخ الأحداث: | Date Created: 20211109 Latest Revision: 20231108 |
| رمز التحديث: | 20240628 |
| مُعرف محوري في PubMed: | PMC8576018 |
| DOI: | 10.1038/s41525-021-00256-y |
| PMID: | 34750377 |
| قاعدة البيانات: | MEDLINE |
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