دورية أكاديمية
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Raman spectroscopic analysis of skin as a diagnostic tool for Human African Trypanosomiasis.

التفاصيل البيبلوغرافية
العنوان: Raman spectroscopic analysis of skin as a diagnostic tool for Human African Trypanosomiasis.
المؤلفون: Girard A; Wellcome Centre for Integrative Parasitology, Institute of Biodiversity Animal Health and Comparative Medicine, University of Glasgow, Glasgow, United Kingdom., Cooper A; Wellcome Centre for Integrative Parasitology, Institute of Biodiversity Animal Health and Comparative Medicine, University of Glasgow, Glasgow, United Kingdom., Mabbott S; Department of Biomedical Engineering, Texas A&M University, College Station, Texas, United States of America.; Center for Remote Health Technologies and Systems, Texas A&M Engineering Experimentation Station, College Station, Texas, United States of America., Bradley B; Wellcome Centre for Integrative Parasitology, Institute of Biodiversity Animal Health and Comparative Medicine, University of Glasgow, Glasgow, United Kingdom., Asiala S; Department of Pure and Applied Chemistry, University of Strathclyde, Glasgow, United Kingdom., Jamieson L; Department of Pure and Applied Chemistry, University of Strathclyde, Glasgow, United Kingdom., Clucas C; Wellcome Centre for Integrative Parasitology, Institute of Biodiversity Animal Health and Comparative Medicine, University of Glasgow, Glasgow, United Kingdom., Capewell P; Wellcome Centre for Integrative Parasitology, Institute of Biodiversity Animal Health and Comparative Medicine, University of Glasgow, Glasgow, United Kingdom., Marchesi F; Veterinary Diagnostic Services, School of Veterinary Medicine, University of Glasgow, Glasgow, United Kingdom., Gibbins MP; Wellcome Centre for Integrative Parasitology, Institute of Infection, Immunity and Inflammation, University of Glasgow, Glasgow, United Kingdom., Hentzschel F; Wellcome Centre for Integrative Parasitology, Institute of Infection, Immunity and Inflammation, University of Glasgow, Glasgow, United Kingdom., Marti M; Wellcome Centre for Integrative Parasitology, Institute of Infection, Immunity and Inflammation, University of Glasgow, Glasgow, United Kingdom., Quintana JF; Wellcome Centre for Integrative Parasitology, Institute of Biodiversity Animal Health and Comparative Medicine, University of Glasgow, Glasgow, United Kingdom., Garside P; Institute of Infection, Immunity and Inflammation, University of Glasgow, Glasgow, United Kingdom., Faulds K; Department of Pure and Applied Chemistry, University of Strathclyde, Glasgow, United Kingdom., MacLeod A; Wellcome Centre for Integrative Parasitology, Institute of Biodiversity Animal Health and Comparative Medicine, University of Glasgow, Glasgow, United Kingdom., Graham D; Department of Pure and Applied Chemistry, University of Strathclyde, Glasgow, United Kingdom.
المصدر: PLoS pathogens [PLoS Pathog] 2021 Nov 15; Vol. 17 (11), pp. e1010060. Date of Electronic Publication: 2021 Nov 15 (Print Publication: 2021).
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Public Library of Science Country of Publication: United States NLM ID: 101238921 Publication Model: eCollection Cited Medium: Internet ISSN: 1553-7374 (Electronic) Linking ISSN: 15537366 NLM ISO Abbreviation: PLoS Pathog Subsets: MEDLINE
أسماء مطبوعة: Original Publication: San Francisco, CA : Public Library of Science, c2005-
مواضيع طبية MeSH: Skin/*pathology , Spectrum Analysis, Raman/*methods , Trypanosoma brucei brucei/*physiology , Trypanosoma brucei gambiense/*physiology , Trypanosomiasis, African/*diagnosis, Animals ; Female ; Mice ; Mice, Inbred BALB C ; Mice, Inbred C57BL ; Skin/parasitology ; Trypanosomiasis, African/parasitology
مستخلص: Human African Trypanosomiasis (HAT) has been responsible for several deadly epidemics throughout the 20th century, but a renewed commitment to disease control has significantly reduced new cases and motivated a target for the elimination of Trypanosoma brucei gambiense-HAT by 2030. However, the recent identification of latent human infections, and the detection of trypanosomes in extravascular tissues hidden from current diagnostic tools, such as the skin, has added new complexity to identifying infected individuals. New and improved diagnostic tests to detect Trypanosoma brucei infection by interrogating the skin are therefore needed. Recent advances have improved the cost, sensitivity and portability of Raman spectroscopy technology for non-invasive medical diagnostics, making it an attractive tool for gambiense-HAT detection. The aim of this work was to assess and develop a new non-invasive diagnostic method for T. brucei through Raman spectroscopy of the skin. Infections were performed in an established murine disease model using the animal-infective Trypanosoma brucei brucei subspecies. The skin of infected and matched control mice was scrutinized ex vivo using a confocal Raman microscope with 532 nm excitation and in situ at 785 nm excitation with a portable field-compatible instrument. Spectral evaluation and Principal Component Analysis confirmed discrimination of T. brucei-infected from uninfected tissue, and a characterisation of biochemical changes in lipids and proteins in parasite-infected skin indicated by prominent Raman peak intensities was performed. This study is the first to demonstrate the application of Raman spectroscopy for the detection of T. brucei by targeting the skin of the host. The technique has significant potential to discriminate between infected and non-infected tissue and could represent a unique, non-invasive diagnostic tool in the goal for elimination of gambiense-HAT as well as for Animal African Trypanosomiasis (AAT).
Competing Interests: The authors have declared that no competing interests exist.
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معلومات مُعتمدة: United Kingdom WT_ Wellcome Trust; 209511/Z/17/Z United Kingdom WT_ Wellcome Trust
تواريخ الأحداث: Date Created: 20211115 Date Completed: 20211231 Latest Revision: 20211231
رمز التحديث: 20221213
مُعرف محوري في PubMed: PMC8629383
DOI: 10.1371/journal.ppat.1010060
PMID: 34780575
قاعدة البيانات: MEDLINE
الوصف
تدمد:1553-7374
DOI:10.1371/journal.ppat.1010060