دورية أكاديمية
Understanding the mode of inhibition and molecular interaction of taxifolin with human adenosine deaminase.
| العنوان: | Understanding the mode of inhibition and molecular interaction of taxifolin with human adenosine deaminase. |
|---|---|
| المؤلفون: | Rawat RS; Centre for Bioseparation Technology (CBST), Vellore Institute of Technology (VIT), Vellore, India., Kumar S; Centre for Bioseparation Technology (CBST), Vellore Institute of Technology (VIT), Vellore, India. |
| المصدر: | Journal of biomolecular structure & dynamics [J Biomol Struct Dyn] 2023 Feb; Vol. 41 (2), pp. 377-385. Date of Electronic Publication: 2021 Dec 01. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Taylor & Francis Country of Publication: England NLM ID: 8404176 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1538-0254 (Electronic) Linking ISSN: 07391102 NLM ISO Abbreviation: J Biomol Struct Dyn Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: June 2012- : Oxon, UK : Taylor & Francis Original Publication: Guilderland, NY : Adenine Press, [c1983- |
| مواضيع طبية MeSH: | Adenosine Deaminase*/chemistry , Adenosine Deaminase*/metabolism , Diabetes Mellitus, Type 2*, Humans ; Pentostatin/pharmacology ; Adenosine Deaminase Inhibitors/pharmacology ; Adenosine Deaminase Inhibitors/chemistry |
| مستخلص: | Adenosine deaminase is a zinc +2 dependent key enzyme of purine metabolism which irreversibly converts adenosine to inosine and form ammonia. Overexpression of adenosine deaminase has been linked to a variety of pathophysiological conditions such as atherosclerosis, hypertension, and diabetes. In the case of a cell-mediated immune response, ADA is thought to be a marker, particularly in type II diabetes. Deoxycoformycin is the most potent ADA inhibitor that has been discovered so far, but it has several drawbacks, including being toxic and having poor pharmacokinetics. Taxifolin, a flavonoid derived from plants, was discovered to be a potent inhibitor of the human ADA (hADA) enzyme in the current study. Taxifolin bound at the active site of human ADA and showed fifty percent inhibition at a concentration of 400 µM against the enzyme. To better understand the interactions between taxifolin and human ADA, docking and molecular dynamic simulations were performed. In-silico studies using autodock revealed that taxifolin bound in the active site of human ADA with a binding energy of -7.4 kcal mol -1 and a theoretical Ki of 3.7 uM. Comparative analysis indicated that taxifolin and deoxycoformycin share a common binding space in the active site of human ADA and inhibit its catalytic activity similarly. The work emphasises the need of employing taxifolin as a lead chemical in order to produce a more precise and effective inhibitor of the human ADA enzyme with therapeutic potential.Communicated by Ramaswamy H. Sarma. |
| فهرسة مساهمة: | Keywords: Human adenosine deaminase; MD simulation; enzyme activity and taxifolin; inhibition studies; molecular docking |
| المشرفين على المادة: | EC 3.5.4.4 (Adenosine Deaminase) 395575MZO7 (Pentostatin) 9SOB9E3987 (taxifolin) 0 (Adenosine Deaminase Inhibitors) |
| تواريخ الأحداث: | Date Created: 20211201 Date Completed: 20230103 Latest Revision: 20230301 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1080/07391102.2021.2006087 |
| PMID: | 34851227 |
| قاعدة البيانات: | MEDLINE |
PDF full text from Publisher 
Full Text Finder | تدمد: | 1538-0254 |
|---|---|
| DOI: | 10.1080/07391102.2021.2006087 |