دورية أكاديمية
An Ethanolic Extract of Cucurbita pepo L. Seeds Modifies Neuroendocrine Disruption in Chronic Stressed Rats and Adrenal Expression of Inflammatory Markers and HSP70.
| العنوان: | An Ethanolic Extract of Cucurbita pepo L. Seeds Modifies Neuroendocrine Disruption in Chronic Stressed Rats and Adrenal Expression of Inflammatory Markers and HSP70. |
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| المؤلفون: | Almohaimeed HM; Department of Basic Science, College of Medicine, Princess Nourah Bint Abdulrahman University (PNU), Riyadh, Saudi Arabia., Hamed S; Department of Medical Histology and Cell Biology, Faculty of Medicine, Mansoura University, Mansoura, Egypt., Seleem HS; Histology Department, Faculty of Medicine, Menoufia University, Shebin ElKoum, Egypt.; Department of Basic Medical Sciences, Unaizah College of Medicine and Medical Sciences, Qassim University, Buraydah, Saudi Arabia., Batawi AH; Department of Biological Science, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia., Mohammedsaleh ZM; Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences, University of Tabuk, Tabuk, Saudi Arabia., Balgoon MJ; Department of Biochemistry, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia., Ali SS; Department of Histology and Cell Biology, Faculty of Medicine, Assuit University, Asyut, Egypt.; Yousef Abdullatif Jameel Chair of Prophetic Medical Applications (YAJCPMA), Faculty of Medicine, King Abdulaziz University, Jeddah, Saudi Arabia., Al Jaouni S; Yousef Abdullatif Jameel Chair of Prophetic Medical Applications (YAJCPMA), Faculty of Medicine, King Abdulaziz University, Jeddah, Saudi Arabia.; Department of Hematology/Pediatric Oncology, King Abdulaziz University Hospital (KAUH), Faculty of Medicine, King Abdulaziz University, Jeddah, Saudi Arabia., Ayuob N; Department of Medical Histology, Faculty of Medicine, Damietta University, Damietta, Egypt. |
| المصدر: | Frontiers in pharmacology [Front Pharmacol] 2021 Nov 10; Vol. 12, pp. 749766. Date of Electronic Publication: 2021 Nov 10 (Print Publication: 2021). |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Frontiers Media] Country of Publication: Switzerland NLM ID: 101548923 Publication Model: eCollection Cited Medium: Print ISSN: 1663-9812 (Print) Linking ISSN: 16639812 NLM ISO Abbreviation: Front Pharmacol Subsets: PubMed not MEDLINE |
| أسماء مطبوعة: | Original Publication: [Lausanne : Frontiers Media] |
| مستخلص: | Background: Pumpkins ( Cucurbita pepo L.) were described to have antioxidant, anti-inflammatory, anti-fatigue, and antidepressant-like effect. The adrenal gland is an important stress-responsive organ that maintains homeostasis during stress. Objectives: This study aimed to assess the efficacy of the administration of Cucurbita pepo L. (CP) extract in relieving behavioral, biochemical, and structural changes in the adrenal gland induced by exposure to chronic unpredictable mild stress (CUMS) and to explore the mechanism behind this impact. Materials and Methods: Forty male albino rats were divided into 4 groups ( n = 10): control, CUMS, fluoxetine-treated, and CP-treated groups. Behavioral changes, corticosterone level, pro-inflammatory cytokines TNF-α and IL-6, and oxidant/antioxidant profile were assessed in the serum at the end of the experiment. Adrenal glands were processed for histopathological and immunohistochemical assessment. Gene expression of caspase-3 and Ki67 and heat shock protein 70 (HSP70) were assessed in adrenal glands using RT-PCR. Results: The CP extract significantly reduced the corticosterone level ( p < 0.001), immobility time ( p < 0.001), and inflammatory and oxidative changes associated with CUMS-induced depression compared to the untreated group. The CP extract alleviated CUMS-induced adrenal histopathological changes and significantly reduced apoptosis ( p < 0.001) and significantly upregulated antioxidant levels in the serum. Conclusion: Cucurbita pepo L. effectively ameliorated the chronic stress-induced behavioral, biochemical, and adrenal structural changes mostly through its antioxidant and anti-inflammatory effects. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2021 Almohaimeed, Hamed, Seleem, Batawi, Mohammedsaleh, Balgoon, Ali, Al Jaouni and Ayuob.) |
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| فهرسة مساهمة: | Keywords: Hsp70; apoptosis; caspase-3; depression; ki67; pumpkin; stress |
| تواريخ الأحداث: | Date Created: 20211206 Latest Revision: 20211207 |
| رمز التحديث: | 20231215 |
| مُعرف محوري في PubMed: | PMC8636010 |
| DOI: | 10.3389/fphar.2021.749766 |
| PMID: | 34867356 |
| قاعدة البيانات: | MEDLINE |
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