دورية أكاديمية
أعرض في EDS

Functional evaluation of the P681H mutation on the proteolytic activation of the SARS-CoV-2 variant B.1.1.7 (Alpha) spike.

التفاصيل البيبلوغرافية
العنوان: Functional evaluation of the P681H mutation on the proteolytic activation of the SARS-CoV-2 variant B.1.1.7 (Alpha) spike.
المؤلفون: Lubinski B; Department of Microbiology & Immunology, College of Veterinary Medicine, Cornell University, 618 Tower Road, Ithaca, NY 14853, USA., Fernandes MHV; Department of Population Medicine, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA., Frazier L; Department of Microbiology & Immunology, College of Veterinary Medicine, Cornell University, 618 Tower Road, Ithaca, NY 14853, USA., Tang T; Robert Frederick Smith School of Chemical & Biomolecular Engineering, Cornell University, Ithaca, NY 14853, USA., Daniel S; Robert Frederick Smith School of Chemical & Biomolecular Engineering, Cornell University, Ithaca, NY 14853, USA., Diel DG; Department of Population Medicine, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA., Jaimes JA; Department of Microbiology & Immunology, College of Veterinary Medicine, Cornell University, 618 Tower Road, Ithaca, NY 14853, USA., Whittaker GR; Department of Microbiology & Immunology, College of Veterinary Medicine, Cornell University, 618 Tower Road, Ithaca, NY 14853, USA.; Master of Public Health Program, Cornell University, Ithaca, NY 14853, USA.
المصدر: IScience [iScience] 2022 Jan 21; Vol. 25 (1), pp. 103589. Date of Electronic Publication: 2021 Dec 10.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Cell Press Country of Publication: United States NLM ID: 101724038 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2589-0042 (Electronic) Linking ISSN: 25890042 NLM ISO Abbreviation: iScience Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: [Cambridge, MA] : Cell Press, [2018]-
مستخلص: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the agent causing the COVID-19 pandemic. SARS-CoV-2 B.1.1.7 (Alpha), a WHO variant of concern first identified in the United Kingdom in late 2020, contains several mutations including P681H in the spike S1/S2 cleavage site, which is predicted to increase cleavage by furin, potentially impacting the viral cell entry. Here, we studied the role of the P681H mutation in B.1.1.7 cell entry. We performed assays using fluorogenic peptides mimicking the Wuhan-Hu-1 and B.1.1.7 S1/S2 sequence and observed no significant difference in furin cleavage. Functional assays using pseudoparticles harboring SARS-CoV-2 spikes and cell-to-cell fusion assays demonstrated no differences between Wuhan-Hu-1, B.1.1.7, or a P681H point mutant. Likewise, we observed no differences in viral growth between USA-WA1/2020 and a B.1.1.7 isolate in cell culture. Our findings suggest that, although the B.1.1.7 P681H mutation may slightly increase S1/S2 cleavage, this does not significantly impact viral entry or cell-cell spread.
Competing Interests: The authors manifest no conflict of interest.
(© 2021 The Author(s).)
التعليقات: Update of: bioRxiv. 2021 Apr 08;:. (PMID: 33851153)
References: Science. 2021 Dec 10;374(6573):1353-1360. (PMID: 34698504)
Science. 2021 Apr 9;372(6538):. (PMID: 33658326)
Nature. 2021 Apr;592(7852):122-127. (PMID: 33636719)
Nat Med. 2021 May;27(5):917-924. (PMID: 33772244)
J Vis Exp. 2019 Mar 1;(145):. (PMID: 30882796)
Emerg Microbes Infect. 2020 Dec;9(1):1474-1488. (PMID: 32543348)
Cell Rep. 2022 May 17;39(7):110829. (PMID: 35550680)
iScience. 2020 Jun 26;23(6):101212. (PMID: 32512386)
Nat Med. 2021 Mar;27(3):440-446. (PMID: 33531709)
ACS Infect Dis. 2021 Feb 12;7(2):264-272. (PMID: 33432808)
Nature. 2021 May;593(7858):266-269. (PMID: 33767447)
Nat Commun. 2021 Oct 1;12(1):5769. (PMID: 34599175)
Microbiol Spectr. 2022 Aug 31;10(4):e0151422. (PMID: 35766497)
Lancet. 2020 Feb 15;395(10223):514-523. (PMID: 31986261)
Sci Rep. 2012;2:261. (PMID: 22355773)
Clin Microbiol Infect. 2021 Jun;27(6):823-826. (PMID: 33794345)
Curr Opin Virol. 2021 Apr;47:113-120. (PMID: 33744490)
Virology. 2006 Jul 5;350(2):358-69. (PMID: 16519916)
Lancet Microbe. 2021 Feb;2(2):e53-e54. (PMID: 33521708)
Nature. 2021 Apr;592(7854):438-443. (PMID: 33690265)
Protein Eng Des Sel. 2004 Jan;17(1):107-12. (PMID: 14985543)
JAMA. 2021 Apr 6;325(13):1324-1326. (PMID: 33571356)
Nature. 2021 Mar;591(7849):293-299. (PMID: 33494095)
J Vis Exp. 2019 Jan 9;(143):. (PMID: 30688313)
Hawaii J Health Soc Welf. 2021 Mar 1;80(3):52-61. (PMID: 33718878)
J Mol Biol. 2020 May 1;432(10):3309-3325. (PMID: 32320687)
Nat Rev Drug Discov. 2012 May;11(5):367-83. (PMID: 22679642)
Proc Natl Acad Sci U S A. 2009 Apr 7;106(14):5871-6. (PMID: 19321428)
BMJ. 2021 Mar 9;372:n579. (PMID: 33687922)
JAMA. 2021 Feb 9;325(6):529-531. (PMID: 33404586)
Commun Med (Lond). 2021 Nov 15;1:48. (PMID: 35602219)
فهرسة مساهمة: Keywords: Virology
تواريخ الأحداث: Date Created: 20211215 Latest Revision: 20231108
رمز التحديث: 20231108
مُعرف محوري في PubMed: PMC8662955
DOI: 10.1016/j.isci.2021.103589
PMID: 34909610
قاعدة البيانات: MEDLINE
الوصف
تدمد:2589-0042
DOI:10.1016/j.isci.2021.103589