دورية أكاديمية
The possible alleviating effect of garlic supplement on the neural retina in a rat model of hypercholesterolemia: a histological and immunohistochemical study.
| العنوان: | The possible alleviating effect of garlic supplement on the neural retina in a rat model of hypercholesterolemia: a histological and immunohistochemical study. |
|---|---|
| المؤلفون: | Arafat EA; Histology and Cell Biology Department, Faculty of Medicine, Mansoura University, Mansoura. arafateetmad@gmail.com., Youssef EMI; Department of Biochemistry, Faculty of Medicine, Al-Azhar University, Cairo. emyoussef4@gmail.com., Khalaf HA; Histology and Cell Biology Department, Faculty of Medicine, Mansoura University, Mansoura. ok67203964949@yahoo.com. |
| المصدر: | European journal of histochemistry : EJH [Eur J Histochem] 2021 Dec 16; Vol. 65 (4). Date of Electronic Publication: 2021 Dec 16. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: PagePress Publications Country of Publication: Italy NLM ID: 9207930 Publication Model: Electronic Cited Medium: Internet ISSN: 2038-8306 (Electronic) Linking ISSN: 1121760X NLM ISO Abbreviation: Eur J Histochem Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Jan. 2010- : Pavia : PagePress Publications Original Publication: Pavia : Luigi Ponzio, [1992- |
| مواضيع طبية MeSH: | Anti-Inflammatory Agents/*therapeutic use , Antioxidants/*therapeutic use , Neurodegenerative Diseases/*drug therapy , Plant Extracts/*therapeutic use , Retina/*drug effects , Retinal Degeneration/*drug therapy, Animals ; Apoptosis/drug effects ; Cytokines/metabolism ; Diet, High-Fat ; Dietary Supplements ; Garlic/chemistry ; Hypercholesterolemia/complications ; Immunohistochemistry ; Male ; Neurodegenerative Diseases/etiology ; Oxidative Stress/drug effects ; Rats, Sprague-Dawley ; Retina/pathology ; Retinal Degeneration/etiology ; Retinal Neurons/drug effects ; Rats |
| مستخلص: | The purpose of this work was to prove that oxidative stress is the main mechanism responsible for retinal neurodegenerative changes, subsequent apoptosis, and inflammatory cytokine release in rats fed with a high cholesterol diet (HCD) and determine the role of garlic in alleviating these changes. Forty rats were equally divided into four groups: control, garlic-treated (positive control), HCD, and HCD + garlic-treated (HCD + G). By the end of the experiment (24 weeks) blood samples were collected for assessment of serum lipid profile, oxidative stress parameters, and plasma levels of IL-6 and TNF-α. Both eyes of the rats were enucleated; one was used for light microscopic examination and the other for electron microscopic examination. There was a significant increase in the levels of serum lipids, oxidative stress parameters, IL-6 and TNF-α, and area of expression of caspase-3 in the HCD group compared to both the control and HCD + G groups. Histological examination revealed degenerative changes in all layers of the neural retina in the HCD group. Garlic administration resulted in a significant improvement in the biochemical, immunohistochemical, and histological characteristics of hypercholesterolemic rats. These findings support the hypotheses that garlic has strong antioxidant, anti-apoptotic, and anti-inflammatory properties. Garlic ameliorates the neurodegenerative changes in the neural retina of hypercholesteremic rats. |
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| المشرفين على المادة: | 0 (Anti-Inflammatory Agents) 0 (Antioxidants) 0 (Cytokines) 0 (Plant Extracts) |
| تواريخ الأحداث: | Date Created: 20211216 Date Completed: 20220221 Latest Revision: 20240226 |
| رمز التحديث: | 20240226 |
| مُعرف محوري في PubMed: | PMC8696193 |
| DOI: | 10.4081/ejh.2021.3322 |
| PMID: | 34911286 |
| قاعدة البيانات: | MEDLINE |
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