دورية أكاديمية
أعرض في EDS

The antioxidant and anti-inflammatory effects of Carica Papaya Linn. seeds extract on CCl 4 -induced liver injury in male rats.

التفاصيل البيبلوغرافية
العنوان: The antioxidant and anti-inflammatory effects of Carica Papaya Linn. seeds extract on CCl 4 -induced liver injury in male rats.
المؤلفون: Shaban NZ; Department of Biochemistry, Faculty of Science, Alexandria University, Alexandria, 21511, Egypt. nshaban2001@yahoo.co.uk., El-Kot SM; Department of Biochemistry, Faculty of Science, Alexandria University, Alexandria, 21511, Egypt., Awad OM; Department of Biochemistry, Faculty of Science, Alexandria University, Alexandria, 21511, Egypt., Hafez AM; Department of Environmental Studies, Institute of Graduate Studies and Research, Alexandria University, Alexandria, Egypt., Fouad GM; Department of Histology and Cell Biology, Faculty of Medicine, Alexandria University, Alexandria, Egypt.
المصدر: BMC complementary medicine and therapies [BMC Complement Med Ther] 2021 Dec 30; Vol. 21 (1), pp. 302. Date of Electronic Publication: 2021 Dec 30.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: BioMed Central Country of Publication: England NLM ID: 101761232 Publication Model: Electronic Cited Medium: Internet ISSN: 2662-7671 (Electronic) Linking ISSN: 26627671 NLM ISO Abbreviation: BMC Complement Med Ther Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : BioMed Central, [2020]-
مواضيع طبية MeSH: Carica* , Seeds*, Anti-Inflammatory Agents/*pharmacology , Antioxidants/*pharmacology , Chemical and Drug Induced Liver Injury/*drug therapy , Liver/*drug effects , Plant Extracts/*pharmacology, Animals ; Apoptosis/drug effects ; Biomarkers/analysis ; Carbon Tetrachloride ; Disease Models, Animal ; Kidney Function Tests ; Liver Function Tests ; Male ; Oxidative Stress/drug effects ; Rats ; Rats, Sprague-Dawley
مستخلص: Background: Oxidative stress (OS) and inflammation are the central pathogenic events in liver diseases. In this study, the protective and therapeutic role of Carica Papaya Linn. seeds extract (SE) was evaluated against the hepatotoxicity induced by carbon tetrachloride (CCl 4 ) in rats.
Methods: The air-dried papaya seeds were powdered and extracted with distilled water. The phytochemical ingredients, minerals, and antioxidant potentials were studied. For determination of the biological role of SE against hepatotoxicity induced by CCl 4 , five groups of adult male Sprague-Dawley rats were prepared (8 rats per each): C: control; SE: rats were administered with SE alone; CCl 4 : rats were injected subcutaneously with CCl 4 ; SE-CCl 4 group: rats were administered with SE orally for 2 weeks before and 8 weeks during CCl 4 injection; SE-CCl 4 -SE group: Rats were administered with SE and CCl 4 as mentioned in SE-CCl 4 group with a prolonged administration with SE for 4 weeks after the stopping of CCl 4 injection. Then, the markers of OS [lipid peroxidation (LP) and antioxidant parameters; glutathione (GSH), superoxide dismutase (SOD), glutathione-S-transferase (GST), glutathione peroxidase (GPx)], inflammation [nuclear factor (NF)-κB, tumor necrosis factor (TNF)-α, interleukin (IL)-6], fibrosis [transforming growth factor (TGF)-β], apoptosis [tumor suppressor gene (p53)], liver and kidney functions beside liver histopathology were determined.
Results: The phytochemical analyses revealed that SE contains different concentrations of phenolics, flavonoids, terpenoids, and minerals so it has potent antioxidant activities. Therefore, the treatment with SE pre, during, and/or after CCl 4 administration attenuated the OS induced by CCl 4 where the LP was reduced, but the antioxidants (GSH, SOD, GST, and GPx) were increased. Additionally, these treatments reduced the inflammation, fibrosis, and apoptosis induced by CCl 4 , since the levels of NF-κB, TNF-α, IL-6, TGF-β, and p53 were declined. Accordingly, liver and kidney functions were improved. These results were confirmed by the histopathological results.
Conclusions: SE has protective and treatment roles against hepatotoxicity caused by CCl 4 administration through the reduction of OS, inflammation, fibrosis, and apoptosis induced by CCl 4 and its metabolites in the liver tissues. Administration of SE for healthy rats for 12 weeks had no adverse effects. Thus, SE can be utilized in pharmacological tools as anti-hepatotoxicity.
(© 2021. The Author(s).)
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فهرسة مساهمة: Keywords: Apoptosis; CCl4; Carica Papaya Linn. seeds extract; Inflammation; Liver injury; Oxidative stress
المشرفين على المادة: 0 (Anti-Inflammatory Agents)
0 (Antioxidants)
0 (Biomarkers)
0 (Plant Extracts)
CL2T97X0V0 (Carbon Tetrachloride)
تواريخ الأحداث: Date Created: 20211231 Date Completed: 20220110 Latest Revision: 20220110
رمز التحديث: 20240829
مُعرف محوري في PubMed: PMC8719404
DOI: 10.1186/s12906-021-03479-9
PMID: 34969385
قاعدة البيانات: MEDLINE
الوصف
تدمد:2662-7671
DOI:10.1186/s12906-021-03479-9