دورية أكاديمية
An isotopic labeling approach linking natural products with biosynthetic gene clusters.
| العنوان: | An isotopic labeling approach linking natural products with biosynthetic gene clusters. |
|---|---|
| المؤلفون: | McCaughey CS; Department of Chemistry, Simon Fraser University, Burnaby, British Columbia, Canada., van Santen JA; Department of Chemistry, Simon Fraser University, Burnaby, British Columbia, Canada., van der Hooft JJJ; Bioinformatics Group, Wageningen University, Wageningen, the Netherlands., Medema MH; Bioinformatics Group, Wageningen University, Wageningen, the Netherlands., Linington RG; Department of Chemistry, Simon Fraser University, Burnaby, British Columbia, Canada. rliningt@sfu.ca. |
| المصدر: | Nature chemical biology [Nat Chem Biol] 2022 Mar; Vol. 18 (3), pp. 295-304. Date of Electronic Publication: 2021 Dec 30. |
| نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Nature Pub. Group Country of Publication: United States NLM ID: 101231976 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1552-4469 (Electronic) Linking ISSN: 15524450 NLM ISO Abbreviation: Nat Chem Biol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: New York, NY : Nature Pub. Group, [2005]- |
| مواضيع طبية MeSH: | Biological Products* , Micromonospora*, Biosynthetic Pathways/genetics ; Isotope Labeling ; Multigene Family |
| مستخلص: | Major advances in genome sequencing and large-scale biosynthetic gene cluster (BGC) analysis have prompted an age of natural product discovery driven by genome mining. Still, connecting molecules to their cognate BGCs is a substantial bottleneck for this approach. We have developed a mass-spectrometry-based parallel stable isotope labeling platform, termed IsoAnalyst, which assists in associating metabolite stable isotope labeling patterns with BGC structure prediction to connect natural products to their corresponding BGCs. Here we show that IsoAnalyst can quickly associate both known metabolites and unknown analytes with BGCs to elucidate the complex chemical phenotypes of these biosynthetic systems. We validate this approach for a range of compound classes, using both the type strain Saccharopolyspora erythraea and an environmentally isolated Micromonospora sp. We further demonstrate the utility of this tool with the discovery of lobosamide D, a new and structurally unique member of the family of lobosamide macrolactams. (© 2021. The Author(s), under exclusive licence to Springer Nature America, Inc.) |
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| معلومات مُعتمدة: | U41 AT008718 United States AT NCCIH NIH HHS |
| المشرفين على المادة: | 0 (Biological Products) |
| تواريخ الأحداث: | Date Created: 20211231 Date Completed: 20220419 Latest Revision: 20230211 |
| رمز التحديث: | 20230212 |
| مُعرف محوري في PubMed: | PMC8891042 |
| DOI: | 10.1038/s41589-021-00949-6 |
| PMID: | 34969972 |
| قاعدة البيانات: | MEDLINE |
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