دورية أكاديمية
Cryo-EM analysis of Ebola virus nucleocapsid-like assembly.
| العنوان: | Cryo-EM analysis of Ebola virus nucleocapsid-like assembly. |
|---|---|
| المؤلفون: | Wang Y; The State Key Laboratory of Biotherapy and Cancer Center, Department of Geriatrics and National Clinical Research Center for Geriatrics, West China Hospital, Sichuan University, Chengdu, Sichuan 610044, China., Binning JM; Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, MO 63110, USA.; Department of Molecular Oncology, H. Lee Moffitt Cancer Center and Research Institute, Tampa, FL 33612, USA., Pintilie GD; Department of Bioengineering and Department of Microbiology and Immunology, James H. Clark Center, Stanford University, Stanford, CA 94305, USA.; Division of Cryo-EM and Bioimaging, SSRL, SLAC National Accelerator Laboratory, Menlo Park, CA 94025, USA., Chiu W; Department of Bioengineering and Department of Microbiology and Immunology, James H. Clark Center, Stanford University, Stanford, CA 94305, USA.; Division of Cryo-EM and Bioimaging, SSRL, SLAC National Accelerator Laboratory, Menlo Park, CA 94025, USA., Amarasinghe GK; Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, MO 63110, USA., Leung DW; Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, MO 63110, USA.; Department of Medicine, Washington University School of Medicine, St. Louis, MO 63110, USA., Su Z; The State Key Laboratory of Biotherapy and Cancer Center, Department of Geriatrics and National Clinical Research Center for Geriatrics, West China Hospital, Sichuan University, Chengdu, Sichuan 610044, China. |
| المصدر: | STAR protocols [STAR Protoc] 2021 Dec 16; Vol. 3 (1), pp. 101030. Date of Electronic Publication: 2021 Dec 16 (Print Publication: 2022). |
| نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Cell Press Country of Publication: United States NLM ID: 101769501 Publication Model: eCollection Cited Medium: Internet ISSN: 2666-1667 (Electronic) Linking ISSN: 26661667 NLM ISO Abbreviation: STAR Protoc Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: [Cambridge, MA] : Cell Press, [2020]- |
| مواضيع طبية MeSH: | Ebolavirus*/chemistry , Hemorrhagic Fever, Ebola*, Cryoelectron Microscopy/methods ; Humans ; Nucleocapsid/chemistry ; Virus Assembly |
| مستخلص: | This protocol describes the reconstitution of the filamentous Ebola virus nucleocapsid-like assembly in vitro . This is followed by solving the cryo-EM structure using helical reconstruction, and flexible fitting of the existing model into the 5.8 Å cryo-EM map. The protocol can be applied to other filamentous viral protein assemblies, particularly those with high flexibility and moderate resolution maps, which present technical challenges to model building. For complete details on the use and execution of this profile, please refer to Su et al. (2018). Competing Interests: The authors declare no competing interests. (© 2021 The Author(s).) |
| References: | Methods. 2009 Oct;49(2):174-80. (PMID: 19398010) J Struct Biol. 1999 Dec 1;128(1):82-97. (PMID: 10600563) J Comput Chem. 2004 Oct;25(13):1605-12. (PMID: 15264254) Nature. 2018 Nov;563(7729):137-140. (PMID: 30333622) Cell Rep. 2015 Jul 7;12(1):140-149. (PMID: 26119732) J Struct Biol. 2017 Jun;198(3):163-176. (PMID: 28193500) J Electron Microsc Tech. 1988 Aug;9(4):325-58. (PMID: 3058895) Biophys J. 2016 Feb 23;110(4):827-39. (PMID: 26743049) Cell Rep. 2015 Apr 21;11(3):376-89. (PMID: 25865894) J Struct Biol. 2012 Dec;180(3):519-30. (PMID: 23000701) J Struct Biol. 2007 Jan;157(1):38-46. (PMID: 16859925) J Struct Biol. 2007 Jan;157(1):83-94. (PMID: 16919474) J Struct Biol. 2015 Nov;192(2):216-21. (PMID: 26278980) Bioinformatics. 2000 Apr;16(4):404-5. (PMID: 10869041) Proteomics. 2009 Mar;9(6):1548-55. (PMID: 19235167) Science. 2021 Aug 20;373(6557):871-876. (PMID: 34282049) J Vis Exp. 2011 Dec 01;(58):. (PMID: 22158337) Nature. 2021 Aug;596(7873):583-589. (PMID: 34265844) Nat Methods. 2013 Jun;10(6):584-90. (PMID: 23644547) J Struct Biol. 2010 Jun;170(3):427-38. (PMID: 20338243) Biochem Soc Trans. 1996 Feb;24(1):274-9. (PMID: 8674685) Cell. 2018 Feb 22;172(5):966-978.e12. (PMID: 29474922) |
| معلومات مُعتمدة: | P41 GM103832 United States GM NIGMS NIH HHS; R01 GM079429 United States GM NIGMS NIH HHS; P01 AI120943 United States AI NIAID NIH HHS; R01 AI123926 United States AI NIAID NIH HHS; R01 AI107056 United States AI NIAID NIH HHS; S10 OD021600 United States OD NIH HHS |
| فهرسة مساهمة: | Keywords: Cryo-EM; Microbiology; Protein Biochemistry; Protein expression and purification; Structural Biology |
| تواريخ الأحداث: | Date Created: 20220103 Date Completed: 20220408 Latest Revision: 20220716 |
| رمز التحديث: | 20240829 |
| مُعرف محوري في PubMed: | PMC8689349 |
| DOI: | 10.1016/j.xpro.2021.101030 |
| PMID: | 34977676 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 2666-1667 |
|---|---|
| DOI: | 10.1016/j.xpro.2021.101030 |