دورية أكاديمية
Proteomics of isolated sieve tubes from Nicotiana tabacum : sieve element-specific proteins reveal differentiation of the endomembrane system.
| العنوان: | Proteomics of isolated sieve tubes from Nicotiana tabacum : sieve element-specific proteins reveal differentiation of the endomembrane system. |
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| المؤلفون: | Liu Y; School of Biological Sciences, Washington State University, Pullman, WA 99154., Vasina VV; School of Biological Sciences, Washington State University, Pullman, WA 99154., Kraner ME; Division of Biochemistry, Department of Biology, Friedrich-Alexander-University Erlangen-Nuremberg, 91058 Erlangen, Germany., Peters WS; School of Biological Sciences, Washington State University, Pullman, WA 99154.; Department of Biology, Purdue University Fort Wayne, Fort Wayne, IN 46835., Sonnewald U; Division of Biochemistry, Department of Biology, Friedrich-Alexander-University Erlangen-Nuremberg, 91058 Erlangen, Germany., Knoblauch M; School of Biological Sciences, Washington State University, Pullman, WA 99154; knoblauch@wsu.edu. |
| المصدر: | Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2022 Jan 04; Vol. 119 (1). |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: National Academy of Sciences Country of Publication: United States NLM ID: 7505876 Publication Model: Print Cited Medium: Internet ISSN: 1091-6490 (Electronic) Linking ISSN: 00278424 NLM ISO Abbreviation: Proc Natl Acad Sci U S A Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Washington, DC : National Academy of Sciences |
| مواضيع طبية MeSH: | Proteomics*, Endoplasmic Reticulum/*metabolism , Plant Cells/*metabolism , Plant Leaves/*metabolism , Plant Stems/*metabolism , Plants, Genetically Modified/*metabolism , Nicotiana/*metabolism, Endoplasmic Reticulum/genetics ; Plant Leaves/cytology ; Plant Leaves/genetics ; Plant Stems/cytology ; Plant Stems/genetics ; Plants, Genetically Modified/cytology ; Plants, Genetically Modified/genetics ; Nicotiana/cytology ; Nicotiana/genetics |
| مستخلص: | Symplasmicly connected cells called sieve elements form a network of tubes in the phloem of vascular plants. Sieve elements have essential functions as they provide routes for photoassimilate distribution, the exchange of developmental signals, and the coordination of defense responses. Nonetheless, they are the least understood main type of plant cells. They are extremely sensitive, possess a reduced endomembrane system without Golgi apparatus, and lack nuclei and translation machineries, so that transcriptomics and similar techniques cannot be applied. Moreover, the analysis of phloem exudates as a proxy for sieve element composition is marred by methodological problems. We developed a simple protocol for the isolation of sieve elements from leaves and stems of Nicotiana tabacum at sufficient amounts for large-scale proteome analysis. By quantifying the enrichment of individual proteins in purified sieve element relative to bulk phloem preparations, proteins of increased likelyhood to function specifically in sieve elements were identified. To evaluate the validity of this approach, yellow fluorescent protein constructs of genes encoding three of the candidate proteins were expressed in plants. Tagged proteins occurred exclusively in sieve elements. Two of them, a putative cytochrome b561/ferric reductase and a reticulon-like protein, appeared restricted to segments of the endoplasmic reticulum (ER) that were inaccessible to green fluorescent protein dissolved in the ER lumen, suggesting a previously unknown differentiation of the endomembrane system in sieve elements. Evidently, our list of promising candidate proteins ( SI Appendix , Table S1 ) provides a valuable exploratory tool for sieve element biology. Competing Interests: The authors declare no competing interest. (Copyright © 2021 the Author(s). Published by PNAS.) |
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| فهرسة مساهمة: | Keywords: phloem; proteomics; sieve element; sieve tube endomembrane system |
| تواريخ الأحداث: | Date Created: 20220105 Date Completed: 20220221 Latest Revision: 20240405 |
| رمز التحديث: | 20240405 |
| مُعرف محوري في PubMed: | PMC8740716 |
| DOI: | 10.1073/pnas.2112755119 |
| PMID: | 34983847 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1091-6490 |
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| DOI: | 10.1073/pnas.2112755119 |