دورية أكاديمية
Macrophage Polarization and Alveolar Bone Healing Outcome: Despite a Significant M2 Polarizing Effect, VIP and PACAP Treatments Present a Minor Impact in Alveolar Bone Healing in Homeostatic Conditions.
العنوان: | Macrophage Polarization and Alveolar Bone Healing Outcome: Despite a Significant M2 Polarizing Effect, VIP and PACAP Treatments Present a Minor Impact in Alveolar Bone Healing in Homeostatic Conditions. |
---|---|
المؤلفون: | Azevedo MCS; Bauru School of Dentistry, Department of Biological Sciences, University of São Paulo, Bauru, Brazil., Fonseca AC; Bauru School of Dentistry, Department of Biological Sciences, University of São Paulo, Bauru, Brazil., Colavite PM; Bauru School of Dentistry, Department of Biological Sciences, University of São Paulo, Bauru, Brazil., Melchiades JL; Bauru School of Dentistry, Department of Biological Sciences, University of São Paulo, Bauru, Brazil., Tabanez AP; Bauru School of Dentistry, Department of Biological Sciences, University of São Paulo, Bauru, Brazil., Codo AC; School of Pharmaceutical Sciences, Department of Immunology, São Paulo State University, Araraquara, Brazil., de Medeiros AI; School of Pharmaceutical Sciences, Department of Immunology, São Paulo State University, Araraquara, Brazil., Trombone APF; Department of Health Sciences, Centro Universitário Sagrado Coração (UNISAGRADO), Bauru, Brazil., Garlet GP; Bauru School of Dentistry, Department of Biological Sciences, University of São Paulo, Bauru, Brazil. |
المصدر: | Frontiers in immunology [Front Immunol] 2021 Dec 21; Vol. 12, pp. 782566. Date of Electronic Publication: 2021 Dec 21 (Print Publication: 2021). |
نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
اللغة: | English |
بيانات الدورية: | Publisher: Frontiers Research Foundation] Country of Publication: Switzerland NLM ID: 101560960 Publication Model: eCollection Cited Medium: Internet ISSN: 1664-3224 (Electronic) Linking ISSN: 16643224 NLM ISO Abbreviation: Front Immunol Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: [Lausanne : Frontiers Research Foundation] |
مواضيع طبية MeSH: | Alveolar Process/*injuries , Macrophage Activation/*drug effects , Pituitary Adenylate Cyclase-Activating Polypeptide/*administration & dosage , Vasoactive Intestinal Peptide/*administration & dosage , Wound Healing/*immunology, Alveolar Process/drug effects ; Alveolar Process/immunology ; Alveolar Process/surgery ; Animals ; Cell Differentiation/drug effects ; Cell Differentiation/immunology ; Disease Models, Animal ; Female ; Immunomodulation/drug effects ; Male ; Mice ; Osteoblasts/physiology ; Osteogenesis/drug effects ; Osteogenesis/immunology ; Tooth Extraction/adverse effects ; Wound Healing/drug effects ; X-Ray Microtomography |
مستخلص: | Host inflammatory immune response comprises an essential element of the bone healing process, where M2 polarization allegedly contributes to a favorable healing outcome. In this context, immunoregulatory molecules that modulate host response, including macrophage polarization, are considered potential targets for improving bone healing. This study aims to evaluate the role of the immunoregulatory molecules VIP (Vasoactive intestinal peptide) and PACAP (Pituitary adenylate cyclase activating polypeptide), which was previously described to favor the development of the M2 phenotype, in the process of alveolar bone healing in C57Bl/6 (WT) mice. Experimental groups were submitted to tooth extraction and maintained under control conditions or treated with VIP or PACAP were evaluated by microtomographic (µCT), histomorphometric, immunohistochemical, and molecular analysis at 0, 3, 7, and 14 days to quantify tissue healing and host response indicators at the healing site. Gene expression analysis demonstrates the effectiveness of VIP or PACAP in modulating host response, evidenced by the early dominance of an M2-type response, which was paralleled by a significant increase in M2 (CD206 + ) in treated groups. However, despite the marked effect of M1/M2 balance in the healing sites, the histomorphometric analysis does not reveal an equivalent/corresponding modulation of the healing process. µCT reveals a slight increase in bone matrix volume and the trabecular thickness number in the PACAP group, while histomorphometric analyzes reveal a slight increase in the VIP group, both at a 14-d time-point; despite the increased expression of osteogenic factors, osteoblastic differentiation, activity, and maturation markers in both VIP and PACAP groups. Interestingly, a lower number of VIP and PACAP immunolabeled cells were observed in the treated groups, suggesting a reduction in endogenous production. In conclusion, while both VIP and PACAP treatments presented a significant immunomodulatory effect with potential for increased healing, no major changes were observed in bone healing outcome, suggesting that the signals required for bone healing under homeostatic conditions are already optimal, and additional signals do not improve an already optimal process. Further studies are required to elucidate the role of macrophage polarization in the bone healing process. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2021 Azevedo, Fonseca, Colavite, Melchiades, Tabanez, Codo, de Medeiros, Trombone and Garlet.) |
References: | J Dent Res. 2007 Oct;86(10):937-50. (PMID: 17890669) J Periodontal Res. 2005 Aug;40(4):287-93. (PMID: 15966905) Adv Wound Care (New Rochelle). 2013 Sep;2(7):357-368. (PMID: 24587973) Results Probl Cell Differ. 2017;62:245-271. (PMID: 28455712) Iowa Orthop J. 2000;20:49-58. (PMID: 10934625) Mol Med Rep. 2015 May;11(5):3212-8. (PMID: 25572286) Front Immunol. 2020 Jun 04;11:1056. (PMID: 32582170) Microbes Infect. 2001 Feb;3(2):141-7. (PMID: 11251300) J Neurochem. 2007 Feb;100(3):603-16. (PMID: 17181550) Br J Pharmacol. 2012 May;166(1):4-17. (PMID: 22289055) Neuropeptides. 2013 Dec;47(6):451-61. (PMID: 24210136) Acta Biomater. 2017 Apr 15;53:13-28. (PMID: 28119112) J Dermatol Sci. 2014 Jun;74(3):193-203. (PMID: 24630238) Nat Rev Immunol. 2011 Oct 10;11(11):762-74. (PMID: 21984070) Innate Immun. 2019 May;25(4):255-264. (PMID: 30935267) Eur J Pharmacol. 2020 Jun 15;877:173090. (PMID: 32234529) Nat Rev Immunol. 2007 Apr;7(4):292-304. (PMID: 17380158) Pharmacol Rev. 2004 Jun;56(2):249-90. (PMID: 15169929) Arch Immunol Ther Exp (Warsz). 2001;49(2):101-10. (PMID: 11348014) Adv Wound Care (New Rochelle). 2014 Jul 1;3(7):492-501. (PMID: 25032069) Int Arch Allergy Immunol. 2007;143(1):31-48. (PMID: 17191007) J Biomed Sci. 2016 Aug 23;23(1):63. (PMID: 27553659) J Cell Physiol. 2009 Oct;221(1):75-83. (PMID: 19496170) J Leukoc Biol. 2005 May;77(5):729-38. (PMID: 15661828) Exp Physiol. 1997 Sep;82(5):871-86. (PMID: 9331555) Endocrinology. 1983 Apr;112(4):1233-9. (PMID: 6187560) Adv Sci (Weinh). 2021 Oct;8(19):e2100584. (PMID: 34382372) J Clin Pathol. 2008 May;61(5):577-87. (PMID: 18441154) PLoS One. 2015 Oct 28;10(10):e0140848. (PMID: 26509533) J Mol Neurosci. 2021 Aug;71(8):1543-1555. (PMID: 31808034) F1000Prime Rep. 2014 Mar 03;6:13. (PMID: 24669294) Endocrinology. 2004 Mar;145(3):1203-10. (PMID: 14617572) Nat Rev Immunol. 2013 Mar;13(3):159-75. (PMID: 23435331) FASEB J. 1991 May;5(8):2145-54. (PMID: 1850705) Peptides. 2013 Apr;42:105-11. (PMID: 23340020) J Neuroimmunol. 1999 Jan 1;93(1-2):126-38. (PMID: 10378876) J Musculoskelet Neuronal Interact. 2008 Apr-Jun;8(2):154-65. (PMID: 18622084) Blood Rev. 2013 Mar;27(2):77-89. (PMID: 23434399) Thromb Haemost. 2011 May;105 Suppl 1:S13-33. (PMID: 21479340) Exp Gerontol. 2012 Feb;47(2):136-42. (PMID: 22138375) Ann N Y Acad Sci. 2006 Jul;1070:322-9. (PMID: 16888186) Endocrinology. 2001 Jan;142(1):339-47. (PMID: 11145597) Neuropeptides. 2017 Oct;65:114-119. (PMID: 28751044) Nat Med. 2001 May;7(5):563-8. (PMID: 11329057) Nat Rev Immunol. 2008 Dec;8(12):958-69. (PMID: 19029990) Nat Rev Immunol. 2016 May 27;16(6):378-91. (PMID: 27231052) Annu Rev Immunol. 2002;20:197-216. (PMID: 11861602) J Neuroimmunol. 1999 May 3;96(2):167-81. (PMID: 10337915) J Dent Res. 2013 Jun;92(6):553-9. (PMID: 23611925) Trends Mol Med. 2006 Jan;12(1):17-25. (PMID: 16356770) Ann N Y Acad Sci. 2000;921:92-102. (PMID: 11193883) Trends Mol Med. 2008 Jun;14(6):245-53. (PMID: 18468489) Nat Rev Immunol. 2011 Oct 14;11(11):723-37. (PMID: 21997792) J Cell Biochem. 2011 Dec;112(12):3732-41. (PMID: 21815197) Cell Mol Life Sci. 2016 Oct;73(20):3861-85. (PMID: 27180275) J Bone Miner Res. 1992 Sep;7(9):1011-9. (PMID: 1384276) Peptides. 2001 Dec;22(12):2151-4. (PMID: 11786203) J Appl Oral Sci. 2011 May-Jun;19(3):260-8. (PMID: 21625744) Oral Microbiol Immunol. 2006 Feb;21(1):12-20. (PMID: 16390336) Iowa Orthop J. 2004;24:123-32. (PMID: 15296219) J Periodontol. 2003 Mar;74(3):391-401. (PMID: 12710761) Einstein (Sao Paulo). 2015 Jul-Sep;13(3):469-73. (PMID: 26466066) Biochem Biophys Res Commun. 2000 Aug 2;274(2):400-4. (PMID: 10913350) J Cell Physiol. 2018 Sep;233(9):6425-6440. (PMID: 29319160) Nat Med. 2017 Mar 7;23(3):279-287. (PMID: 28267716) Neuroscience. 2009 Jun 30;161(2):413-21. (PMID: 19332106) Science. 2000 Sep 1;289(5484):1504-8. (PMID: 10968780) Int J Mol Sci. 2018 Aug 27;19(9):. (PMID: 30150589) Int Immunopharmacol. 2007 Oct;7(10):1343-9. (PMID: 17673149) Front Biosci. 2008 Jan 01;13:453-61. (PMID: 17981560) J Clin Invest. 2004 Mar;113(6):905-12. (PMID: 15067323) Int J Mol Sci. 2019 Jan 04;20(1):. (PMID: 30621194) Circ Res. 2003 May 2;92(8):827-39. (PMID: 12730128) Bone. 2020 Feb;131:115109. (PMID: 31715336) Neurosci Lett. 1997 Sep 19;233(2-3):125-8. (PMID: 9350848) J Biomed Sci. 2018 Mar 14;25(1):25. (PMID: 29540226) J Immunol Res. 2018 Aug 1;2018:6043710. (PMID: 30155495) Oral Dis. 2002 May;8(3):147-59. (PMID: 12108759) J Immunol. 2000 Jun 15;164(12):6166-73. (PMID: 10843666) J Bone Miner Res. 2019 Mar;34(3):547-556. (PMID: 30347467) Results Probl Cell Differ. 2017;62:3-22. (PMID: 28455703) Curr Pharm Des. 2003;9(6):483-94. (PMID: 12570811) J Bone Miner Res. 2010 Jul;25(7):1468-86. (PMID: 20533309) Angiogenesis. 2014 Jan;17(1):109-18. (PMID: 24013945) Biochem Biophys Res Commun. 2000 Apr 29;271(1):158-63. (PMID: 10777696) Acta Cir Bras. 2011 Oct;26(5):365-72. (PMID: 21952659) Biomaterials. 2015 Jan;37:194-207. (PMID: 25453950) J Clin Invest. 2012 Mar;122(3):787-95. (PMID: 22378047) J Immunol. 1985 Jul;135(1):350-4. (PMID: 2582037) PLoS One. 2015 May 29;10(5):e0128021. (PMID: 26023920) J Photochem Photobiol B. 2003 May-Jun;70(2):81-9. (PMID: 12849698) Crit Rev Oral Biol Med. 2002;13(3):229-37. (PMID: 12090463) Clin Oral Implants Res. 2014 Mar;25(3):288-295. (PMID: 23551527) J Transl Med. 2016 Sep 06;14:258. (PMID: 27599571) J Mol Neurosci. 2018 Sep;66(1):102-113. (PMID: 30105629) Crit Rev Immunol. 2001;21(5):399-425. (PMID: 11942557) Int J Exp Pathol. 2015 Aug;96(4):269-75. (PMID: 25944684) Endocr Rev. 2008 Jun;29(4):403-40. (PMID: 18451259) J Bone Miner Res. 2011 Jul;26(7):1517-32. (PMID: 21305607) Trends Cell Biol. 1999 May;9(5):199-201. (PMID: 10357607) Peptides. 2015 Apr;66:51-7. (PMID: 25701761) Verh K Acad Geneeskd Belg. 2010;72(5-6):239-51. (PMID: 21409952) J Endod. 2019 Oct;45(10):1228-1236. (PMID: 31402064) Cytokine. 2019 Feb;114:47-60. (PMID: 30584949) |
فهرسة مساهمة: | Keywords: M2 macrophages; PACAP; VIP; bone healing; osteoimmunology |
المشرفين على المادة: | 0 (Pituitary Adenylate Cyclase-Activating Polypeptide) 37221-79-7 (Vasoactive Intestinal Peptide) |
تواريخ الأحداث: | Date Created: 20220107 Date Completed: 20220214 Latest Revision: 20231105 |
رمز التحديث: | 20231105 |
مُعرف محوري في PubMed: | PMC8724033 |
DOI: | 10.3389/fimmu.2021.782566 |
PMID: | 34992601 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1664-3224 |
---|---|
DOI: | 10.3389/fimmu.2021.782566 |