دورية أكاديمية
Characterization of testicular macrophage subpopulations in mice.
| العنوان: | Characterization of testicular macrophage subpopulations in mice. |
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| المؤلفون: | Gayer FA; University Medical Center Göttingen, Institute for Cellular and Molecular Immunology, Göttingen, Germany; University Medical Center Göttingen, Clinic of Urology, Göttingen, Germany., Reichardt SD; University Medical Center Göttingen, Institute for Cellular and Molecular Immunology, Göttingen, Germany., Bohnenberger H; University Medical Center Göttingen, Institute of Pathology, Göttingen, Germany., Engelke M; University Medical Center Göttingen, Institute for Cellular and Molecular Immunology, Göttingen, Germany., Reichardt HM; University Medical Center Göttingen, Institute for Cellular and Molecular Immunology, Göttingen, Germany. Electronic address: hreichardt@med.uni-goettingen.de. |
| المصدر: | Immunology letters [Immunol Lett] 2022 Mar; Vol. 243, pp. 44-52. Date of Electronic Publication: 2022 Feb 08. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier/North-Holland Biomedical Press Country of Publication: Netherlands NLM ID: 7910006 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0542 (Electronic) Linking ISSN: 01652478 NLM ISO Abbreviation: Immunol Lett Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Amsterdam, Elsevier/North-Holland Biomedical Press. |
| مواضيع طبية MeSH: | Macrophage Activation* , Testis*, Animals ; Flow Cytometry ; Macrophages ; Male ; Mice ; Mice, Inbred C57BL |
| مستخلص: | Testis is an immune privileged site, a feature that prevents germ cells from eliciting an autoimmune response. Macrophages contribute to this state of tolerance by adopting an immunoregulatory phenotype. Here, we further characterized their features in mice by analyzing surface markers, anatomic localization as well as morphology and function. Testicular macrophages (TMΦ) were stained for various surface receptors, and MHCII and CD206 were found to be most suitable to discriminate between two subpopulations. Our immunohistochemical analysis further confirmed a predominant localization of CD206 + cells in the interstitial space. Imaging flow cytometry revealed that both subtypes of TMΦ differed in size and contrast, and to some extent also in their ability to engulf high-molecular dextran. To investigate whether the polarization of the immune system had any influence on the phenotype of TMΦ, we compared C57BL/6 and BALB/c mice. Importantly, our analysis revealed that the abundance of cells expressing either MHCII or any of the scavenger receptors CD206, CD163 and CD71 differed between both mouse strains. In addition, the presence of the glucocorticoid receptor in macrophages affected the ratio between individual subpopulations, which is consistent with a crucial role of glucocorticoids in macrophage polarization. Collectively, our results indicate that TMΦ are composed in a variable ratio of distinct subsets with characteristic features, which may shape the immune privilege of the testis also in humans. (Copyright © 2022 European Federation of Immunological Societies. Published by Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Glucocorticoids; Imaging flow cytometry; Macrophages; Mice; Testis |
| تواريخ الأحداث: | Date Created: 20220212 Date Completed: 20220425 Latest Revision: 20220425 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1016/j.imlet.2022.02.003 |
| PMID: | 35149127 |
| قاعدة البيانات: | MEDLINE |
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Full Text Finder | تدمد: | 1879-0542 |
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| DOI: | 10.1016/j.imlet.2022.02.003 |