دورية أكاديمية
Propofol and Sevoflurane Alleviate Malignant Biological Behavior and Cisplatin Resistance of Xuanwei Lung Adenocarcinoma by Modulating the Wnt/β-catenin Pathway and PI3K/AKT Pathway.
| العنوان: | Propofol and Sevoflurane Alleviate Malignant Biological Behavior and Cisplatin Resistance of Xuanwei Lung Adenocarcinoma by Modulating the Wnt/β-catenin Pathway and PI3K/AKT Pathway. |
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| المؤلفون: | Quan Y; Department of Anesthesiology, The Third Affiliated Hospital of Kunming Medical University, 650118, 519 Kunzhou Road, Xishan District, Kunming City, Yunnan Province, P.R. China., Li S; Department of Anesthesiology, The Third Affiliated Hospital of Kunming Medical University, 650118, 519 Kunzhou Road, Xishan District, Kunming City, Yunnan Province, P.R. China., Wang Y; Department of Anesthesiology, The Third Affiliated Hospital of Kunming Medical University, 650118, 519 Kunzhou Road, Xishan District, Kunming City, Yunnan Province, P.R. China., Liu G; Department of Anesthesiology, The Third Affiliated Hospital of Kunming Medical University, 650118, 519 Kunzhou Road, Xishan District, Kunming City, Yunnan Province, P.R. China., Lv Z; Department of Anesthesiology, The Third Affiliated Hospital of Kunming Medical University, 650118, 519 Kunzhou Road, Xishan District, Kunming City, Yunnan Province, P.R. China., Wang Z; Department of Anesthesiology, The Third Affiliated Hospital of Kunming Medical University, 650118, 519 Kunzhou Road, Xishan District, Kunming City, Yunnan Province, P.R. China. |
| المصدر: | Anti-cancer agents in medicinal chemistry [Anticancer Agents Med Chem] 2022; Vol. 22 (11), pp. 2098-2108. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Bentham Science Publishers Country of Publication: Netherlands NLM ID: 101265649 Publication Model: Print Cited Medium: Internet ISSN: 1875-5992 (Electronic) Linking ISSN: 18715206 NLM ISO Abbreviation: Anticancer Agents Med Chem Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Amsterdam : Bentham Science Publishers Original Publication: Sharjah, U.A.E. ; San Francisco, CA : Bentham Science Publishers, [2006]- |
| مواضيع طبية MeSH: | Adenocarcinoma of Lung*/drug therapy , Lung Neoplasms*/pathology , Propofol*/pharmacology, Apoptosis ; Cell Line, Tumor ; Cell Proliferation ; China ; Cisplatin/pharmacology ; Cisplatin/therapeutic use ; Gene Expression Regulation, Neoplastic ; Humans ; Phosphatidylinositol 3-Kinases/metabolism ; Proto-Oncogene Proteins c-akt/metabolism ; Sevoflurane/pharmacology ; Sevoflurane/therapeutic use ; Wnt Signaling Pathway ; beta Catenin/metabolism |
| مستخلص: | Background: Recent studies have shown that propofol and sevoflurane, two common anesthetics, can prevent tumor development. The prevalence of lung adenocarcinoma in China is highest in Yunnan Xuanwei, and many patients with lung cancer in this area are often resistant to platinum-based treatments. Objective: The objective of the study was to investigate the effects of propofol and sevoflurane on malignant biological behavior and cisplatin resistance of Xuanwei lung adenocarcinoma. Methods: Herein, XWLC-05/R, a cisplatin-resistant cell line of XWLC-05 cells from Xuanwei lung adenocarcinoma, was constructed. The XWLC-05 cells and XWLC-05/R cells were treated with propofol and sevoflurane singly or as a combination and subjected to CCK-8 assay, clone formation tests, and flow cytometry analysis to assess the proliferation level of cells. The morphology and number of apoptotic bodies in XWLC-05 cells and XWLC-05/R were examined with a transmission electron microscope. The ANNEXIN V-FITC/PI and transwell assays were performed to evaluate apoptosis, invasion, and migration of the cells. Subsequently, we constructed a Xuanwei lung adenocarcinoma xenograft model to investigate the effects of propofol and sevoflurane on the tumorigenicity of XWLC-05 cells in vivo. Results: Treatment with propofol and sevoflurane significantly inhibited proliferation, invasion, migration, and induced apoptosis of XWLC-05 and XWLC-05/R cells. These effects were more pronounced when propofol and sevoflurane were co-incubated with the cells. Moreover, both propofol and sevoflurane significantly inhibited Wnt/β- catenin and PI3K/AKT pathways. Moreover, the two drugs effects suppressed the malignant biological behavior of XWLC-05 cells and XWLC-05/R cells, and this effect was counteracted by 740Y-P (PI3K/AKT pathway activator) and Wnt pathway activator 1 (Wnt/β-catenin pathway activator). In vivo experiments confirmed that propofol and sevoflurane alleviated the tumorigenicity of XWLC-05 cells. Conclusions: In summary, this study shows, for the first time, that propofol and sevoflurane decrease the proliferation, invasion, migration and induce apoptosis of XWLC-05 cells and XWLC-05/R cells by impeding the Wnt/β-catenin and PI3K/AKT signaling pathways, thereby alleviating the development of Xuanwei lung adenocarcinoma in vivo. Moreover, these effects are more pronounced when the two drugs are combined. (Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.) |
| فهرسة مساهمة: | Keywords: PI3K/AKT pathway; Propofol; Wnt/β-catenin pathway; Xuanwei lung adenocarcinoma; cisplatin resistance; malignant biological behavior; sevoflurane |
| المشرفين على المادة: | 0 (beta Catenin) 38LVP0K73A (Sevoflurane) EC 2.7.11.1 (Proto-Oncogene Proteins c-akt) Q20Q21Q62J (Cisplatin) YI7VU623SF (Propofol) |
| تواريخ الأحداث: | Date Created: 20220214 Date Completed: 20220523 Latest Revision: 20220622 |
| رمز التحديث: | 20240829 |
| DOI: | 10.2174/1871520621666211026092405 |
| PMID: | 35152870 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1875-5992 |
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| DOI: | 10.2174/1871520621666211026092405 |