دورية أكاديمية
Analysis of Gliricidia sepium Leaves by MALDI Mass Spectrometry Imaging.
| العنوان: | Analysis of Gliricidia sepium Leaves by MALDI Mass Spectrometry Imaging. |
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| المؤلفون: | Hertel Pereira AC; Pharmaceutical Science Graduate Program, Universidade Vila Velha, Avenida Comissário José Dantas de Melo, 21 - Boa Vista II, Vila Velha - ES 29102-920, Brazil., Auer AC; Pharmaceutical Science Graduate Program, Universidade Vila Velha, Avenida Comissário José Dantas de Melo, 21 - Boa Vista II, Vila Velha - ES 29102-920, Brazil., Biedel L; Pharmaceutical Science Graduate Program, Universidade Vila Velha, Avenida Comissário José Dantas de Melo, 21 - Boa Vista II, Vila Velha - ES 29102-920, Brazil., de Almeida CM; Chemistry Graduate Program, Universidade Federal do Espírito Santo, Av. Fernando Ferrari, 514 - Goiabeiras, Vitória - ES 29075-910, Brazil., Romão W; Chemistry Graduate Program, Universidade Federal do Espírito Santo, Av. Fernando Ferrari, 514 - Goiabeiras, Vitória - ES 29075-910, Brazil., Endringer DC; Pharmaceutical Science Graduate Program, Universidade Vila Velha, Avenida Comissário José Dantas de Melo, 21 - Boa Vista II, Vila Velha - ES 29102-920, Brazil. |
| المصدر: | Journal of the American Society for Mass Spectrometry [J Am Soc Mass Spectrom] 2022 Mar 02; Vol. 33 (3), pp. 573-583. Date of Electronic Publication: 2022 Feb 14. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: ACS Publications Country of Publication: United States NLM ID: 9010412 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-1123 (Electronic) Linking ISSN: 10440305 NLM ISO Abbreviation: J Am Soc Mass Spectrom Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2020- : Washington, DC : ACS Publications Original Publication: New York, NY : Elsevier, c1990- |
| مواضيع طبية MeSH: | Phytochemicals*/analysis , Phytochemicals*/chemistry , Phytochemicals*/toxicity, Fabaceae/*chemistry , Molecular Imaging/*methods , Plant Leaves/*chemistry , Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/*methods, Animals ; Cell Line ; Cell Survival/drug effects ; Cosmetics ; Mice ; Polyphenols/analysis ; Polyphenols/chemistry ; Polyphenols/toxicity |
| مستخلص: | When investigating the potential use of plants as a raw material for an all-natural cosmetic formulation, the main parameters are the chemical composition, antioxidant potential, antimicrobial action, and toxicity. Additionally, the production of natural cosmetics should also consider the availability of primary materials and the environmental and socioeconomic impact. Gliricidia sepium is a species that produces a large amount of plant material, being cultivated in the agroforestry system. However, studies of phytochemical composition and chemical spatial distribution are scarcely using the MALDI MS (matrix-assisted laser desorption ionization mass spectrometry) and MALDI MSI (mass spectrometry imaging) techniques. A methodology was developed to optimize ionization parameters and analysis conditions by evaluating the efficiency of three matrices: α-cyano-4-hydroxycinnamic acid, 2,5-dihydroxybenzoic acid (DHB), and 2-mercaptobenzothiazole in MALDI MS analysis. All results were compared to ESI MS (electrospray ionization mass spectrometry), and afterward, MALDI MSI analysis was performed on the leaf surface. This study showed through phytochemical analysis that G. sepium leaves are composed of polyphenols and tannins, concluding that the methanolic extract had a higher amount of flavonoid content. Four compounds were identified on the leaf surface, and their spatial distribution was analyzed by MALDI MS using DHB as a matrix. Kaempferol, isorhamnetin, and some fatty acids showed potential applicability for cosmetical use. All the extracts presented antioxidant activity or antimicrobial action and no cytotoxicity. Therefore, extracts of G. sepium could be used as raw materials in cosmetics. |
| فهرسة مساهمة: | Keywords: MALDI MSI; cosmetic; nontoxicity; polyphenols |
| المشرفين على المادة: | 0 (Cosmetics) 0 (Phytochemicals) 0 (Polyphenols) |
| تواريخ الأحداث: | Date Created: 20220214 Date Completed: 20220328 Latest Revision: 20220328 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1021/jasms.1c00367 |
| PMID: | 35157449 |
| قاعدة البيانات: | MEDLINE |
Find this article in full text from Springer Verlag. 
Find this issue from the American Chemical Society | تدمد: | 1879-1123 |
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| DOI: | 10.1021/jasms.1c00367 |