دورية أكاديمية
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Evolutionary Sweeps of Subviral Parasites and Their Phage Host Bring Unique Parasite Variants and Disappearance of a Phage CRISPR-Cas System.

التفاصيل البيبلوغرافية
العنوان: Evolutionary Sweeps of Subviral Parasites and Their Phage Host Bring Unique Parasite Variants and Disappearance of a Phage CRISPR-Cas System.
المؤلفون: Angermeyer A; Department of Plant and Microbial Biology, University of California, Berkeleygrid.47840.3f, Berkeley, California, USA., Hays SG; Department of Plant and Microbial Biology, University of California, Berkeleygrid.47840.3f, Berkeley, California, USA., Nguyen MHT; Department of Plant and Microbial Biology, University of California, Berkeleygrid.47840.3f, Berkeley, California, USA., Johura FT; icddr,b, Dhaka, Bangladesh., Sultana M; icddr,b, Dhaka, Bangladesh., Alam M; icddr,b, Dhaka, Bangladesh., Seed KD; Department of Plant and Microbial Biology, University of California, Berkeleygrid.47840.3f, Berkeley, California, USA.; Chan Zuckerberg Biohub, San Francisco, California, USA.
المصدر: MBio [mBio] 2021 Feb 22; Vol. 13 (1), pp. e0308821. Date of Electronic Publication: 2022 Feb 15.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: American Society for Microbiology Country of Publication: United States NLM ID: 101519231 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2150-7511 (Electronic) NLM ISO Abbreviation: mBio Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Washington, D.C. : American Society for Microbiology
مواضيع طبية MeSH: Cholera*/epidemiology , Parasites* , Bacteriophages*/genetics , Vibrio cholerae*/genetics, Animals ; Humans ; CRISPR-Cas Systems ; Retrospective Studies
مستخلص: Vibrio cholerae is a significant threat to global public health in part due to its propensity for large-scale evolutionary sweeps where lineages emerge and are replaced. These sweeps may originate from the Bay of Bengal, where bacteriophage predation and the evolution of antiphage counterdefenses is a recurring theme. The bacteriophage ICP1 is a key predator of epidemic V. cholerae and is notable for acquiring a CRISPR-Cas system to combat PLE, a defensive subviral parasite encoded by its V. cholerae host. Here, we describe the discovery of four previously unknown PLE variants through a retrospective analysis of >3,000 publicly available sequences as well as one additional variant (PLE10) from recent surveillance of cholera patients in Bangladesh. In recent sampling we also observed a lineage sweep of PLE-negative V. cholerae occurring within the patient population in under a year. This shift coincided with a loss of ICP1's CRISPR-Cas system in favor of a previously prevalent PLE-targeting endonuclease called Odn. Interestingly, PLE10 was resistant to ICP1-encoded Odn, yet it was not found in any recent V. cholerae strains. We also identified isolates from within individual patient samples that revealed both mixed PLE(+)/PLE(-) V. cholerae populations and ICP1 strains possessing CRISPR-Cas or Odn with evidence of in situ recombination. These findings reinforce our understanding of the successive nature of V. cholerae evolution and suggest that ongoing surveillance of V. cholerae, ICP1, and PLE in Bangladesh is important for tracking genetic developments relevant to pandemic cholera that can occur over relatively short timescales. IMPORTANCE With 1 to 4 million estimated cases annually, cholera is a disease of serious global concern in regions where access to safe drinking water is limited by inadequate infrastructure, inequity, or natural disaster. The Global Task Force on Cholera Control (GTFCC.org) considers outbreak surveillance to be a primary pillar in the strategy to reduce mortality from cholera worldwide. Therefore, developing a better understanding of temporal evolutionary changes in the causative agent of cholera, Vibrio cholerae, could help in those efforts. The significance of our research is in tracking the genomic shifts that distinguish V. cholerae outbreaks, with specific attention paid to current and historical trends in the arms race between V. cholerae and a cooccurring viral (bacteriophage) predator. Here, we discover additional diversity of a specific phage defense system in epidemic V. cholerae and document the loss of a phage-encoded CRISPR-Cas system, underscoring the dynamic nature of microbial populations across cholera outbreaks.
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معلومات مُعتمدة: R01 AI127652 United States AI NIAID NIH HHS; R01 AI153303 United States AI NIAID NIH HHS
فهرسة مساهمة: Keywords: CRISPR-Cas; Vibrio cholerae; bacteriophages; cholera; evolution
تواريخ الأحداث: Date Created: 20220215 Date Completed: 20230228 Latest Revision: 20230228
رمز التحديث: 20240829
مُعرف محوري في PubMed: PMC8844924
DOI: 10.1128/mbio.03088-21
PMID: 35164562
قاعدة البيانات: MEDLINE
الوصف
تدمد:2150-7511
DOI:10.1128/mbio.03088-21