دورية أكاديمية
Fabrication of a fully integrated paper microdevice for point-of-care testing of infectious disease using Safranin O dye coupled with loop-mediated isothermal amplification.
| العنوان: | Fabrication of a fully integrated paper microdevice for point-of-care testing of infectious disease using Safranin O dye coupled with loop-mediated isothermal amplification. |
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| المؤلفون: | Dinh VP; Department of BioNano Technology, Gachon University, 1342 Seongnam-daero, Sujeong-gu, Seongnam-si, Gyeonggi-do, 13120, South Korea., Lee NY; Department of BioNano Technology, Gachon University, 1342 Seongnam-daero, Sujeong-gu, Seongnam-si, Gyeonggi-do, 13120, South Korea. Electronic address: nylee@gachon.ac.kr. |
| المصدر: | Biosensors & bioelectronics [Biosens Bioelectron] 2022 May 15; Vol. 204, pp. 114080. Date of Electronic Publication: 2022 Feb 09. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Advanced Technology Country of Publication: England NLM ID: 9001289 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-4235 (Electronic) Linking ISSN: 09565663 NLM ISO Abbreviation: Biosens Bioelectron Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Oxford : Elsevier Advanced Technology Original Publication: [Barking, Essex, England] : Elsevier Applied Science, 1989- |
| مواضيع طبية MeSH: | Biosensing Techniques* , COVID-19*/diagnosis , Communicable Diseases* , Escherichia coli O157*/genetics, Humans ; Molecular Diagnostic Techniques ; Nucleic Acid Amplification Techniques ; Phenazines ; Point-of-Care Testing ; SARS-CoV-2/genetics |
| مستخلص: | In this study, we introduce a paper microdevice fully integrating DNA extraction, loop-mediated isothermal amplification (LAMP), and Safranin O-based colorimetric detection of two major infectious pathogens, namely SARS-CoV-2 and Enterococcus faecium. The paper microdevice is composed of two parts: sample and reaction chambers. A sealing film acted as a bottom layer to allow foldable motion for transferring DNA from sample chamber to reaction chamber in a seamless manner. An FTA card was employed in the sample chamber for DNA extraction and purification from bacteria-spiked milk. After LAMP reaction at 65 °C for 30 min, a novel aggregation-based DNA detection was obtained by Safranin O polymerization in the reaction chamber. Specifically, Safranin O underwent polymerization by addition of oxidant to form Safranin O oligomers. The electrostatic interaction between the positively charged Safranin O oligomers and the negatively charged DNA comprising LAMP amplicons resulted in the aggregation with a dark red color. Meanwhile, in the absence of LAMP amplicons, Safranin O oligomers were well dispersed and displayed their original red color. By using Safranin O-based detection, SARS-CoV-2 and E. faecium were successfully identified by naked eye within 60 min, and the limits of detection were 10 -4 ng/μL and 10 2 CFU/mL, respectively. These results indicate that a fully integrated paper microdevice plays an important role in sample-in-answer-out format in the genetic analyses of infectious disease and serves as a rapid tool for controlling the spread of diseases. (Copyright © 2022 Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Aggregation-based DNA detection; Fully integrated paper microdevice; Infectious disease; Multiple pathogen detection; Point-of-care testing; Safranin O |
| المشرفين على المادة: | 0 (Phenazines) XTX0YXU2HV (safranine T) |
| SCR Protocol: | LAMP assay |
| تواريخ الأحداث: | Date Created: 20220217 Date Completed: 20220315 Latest Revision: 20220315 |
| رمز التحديث: | 20240829 |
| DOI: | 10.1016/j.bios.2022.114080 |
| PMID: | 35176649 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1873-4235 |
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| DOI: | 10.1016/j.bios.2022.114080 |