دورية أكاديمية
أعرض في EDS

Synergistic Cytokine Production by ATP and PGE 2 via P2X4 and EP 3 Receptors in Mouse Bone-Marrow-Derived Mast Cells.

التفاصيل البيبلوغرافية
العنوان: Synergistic Cytokine Production by ATP and PGE 2 via P2X4 and EP 3 Receptors in Mouse Bone-Marrow-Derived Mast Cells.
المؤلفون: Obayashi K; Laboratory of Pharmacology, Faculty of Pharmacy, Takasaki University of Health and Welfare, Takasaki 370-0033, Gunma, Japan., Yoshida K; Laboratory of Pharmacology, Faculty of Pharmacy, Takasaki University of Health and Welfare, Takasaki 370-0033, Gunma, Japan., Ito MA; Laboratory of Pharmacology, Faculty of Pharmacy, Takasaki University of Health and Welfare, Takasaki 370-0033, Gunma, Japan., Mori T; Laboratory of Allergy and Immunology, Faculty of Pharmacy, Takasaki University of Health and Welfare, Takasaki 370-0033, Gunma, Japan., Yamamoto K; Department of Biomedical Engineering, Graduate School of Medicine, The University of Tokyo, Tokyo 113-0033, Japan., Imai T; Discovery Research Laboratories, Nippon Chemiphar Co., Ltd., Misato 341-0005, Saitama, Japan., Matsuoka I; Laboratory of Pharmacology, Faculty of Pharmacy, Takasaki University of Health and Welfare, Takasaki 370-0033, Gunma, Japan.
المصدر: Cells [Cells] 2022 Feb 10; Vol. 11 (4). Date of Electronic Publication: 2022 Feb 10.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: MDPI Country of Publication: Switzerland NLM ID: 101600052 Publication Model: Electronic Cited Medium: Internet ISSN: 2073-4409 (Electronic) Linking ISSN: 20734409 NLM ISO Abbreviation: Cells Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Basel, Switzerland : MDPI
مواضيع طبية MeSH: Cell Degranulation* , Cytokines*/metabolism , Mast Cells*/metabolism , Receptors, Prostaglandin E, EP3 Subtype*/metabolism , Receptors, Purinergic P2X4*/metabolism, Adenosine Triphosphate/metabolism ; Animals ; Bone Marrow/metabolism ; Dinoprostone/metabolism ; Gene Expression ; Interleukin-6/metabolism ; Mice ; RNA, Messenger/metabolism
مستخلص: ATP is an important intercellular messenger in the extracellular space. In mast cells (MCs), ATP stimulates the ionotropic P2X4 receptor (P2X4R), resulting in enhanced degranulation and exacerbation of acute allergic reactions. In this study, we investigate whether ATP regulates inflammatory cytokine production in MCs. Gene expression was analyzed by quantitative RT-PCR, and cytokine production was measured using ELISA. The stimulation of mouse bone-marrow-derived MCs (BMMCs) with ATP alone had little effect on cytokine secretion. However, the co-stimulation with prostaglandin (PG) E 2 resulted in a marked increase in the secretion of various cytokines, such as tumor necrosis factor-α, interleukin (IL)-6, and IL-13, accompanied by an increase in their mRNA levels. The effects of ATP were inhibited by P2X4R antagonists and diminished in BMMCs derived from P2X4R-deficient mice, suggesting that P2X4R mediated the reaction. The effects of PGE 2 were mimicked by an EP 3 receptor (EP 3 R) agonist and blocked by an EP 3 R antagonist. The synergistic cytokine mRNA elevations induced by ATP and PGE 2 were blocked by nuclear factor-κB and Ca 2+ -calcineurin signaling inhibitors. Altogether, these results suggest that combining P2X4R and EP 3 R signaling enhances acute degranulation and the subsequent cytokine secretion, exacerbating allergic inflammation.
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معلومات مُعتمدة: Grant-in-Aid for Scientific Research Japan Society for the Promotion of Science
فهرسة مساهمة: Keywords: ATP; EP3 receptor; P2X4 receptor; bone-marrow-derived mast cells; prostaglandin E2
المشرفين على المادة: 0 (Cytokines)
0 (Interleukin-6)
0 (Ptger3 protein, mouse)
0 (RNA, Messenger)
0 (Receptors, Prostaglandin E, EP3 Subtype)
0 (Receptors, Purinergic P2X4)
8L70Q75FXE (Adenosine Triphosphate)
K7Q1JQR04M (Dinoprostone)
تواريخ الأحداث: Date Created: 20220225 Date Completed: 20220408 Latest Revision: 20220531
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC8870111
DOI: 10.3390/cells11040616
PMID: 35203267
قاعدة البيانات: MEDLINE
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