دورية أكاديمية
MiR-612, miR-637, and miR-874 can Regulate VEGFA Expression in Hepatocellular Carcinoma Cell Lines.
| العنوان: | MiR-612, miR-637, and miR-874 can Regulate VEGFA Expression in Hepatocellular Carcinoma Cell Lines. |
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| المؤلفون: | Castanhole-Nunes MMU; Research Unit of Genetics and Molecular Biology (UPGEM), Department of Molecular Biology, Faculty of Medicine of Sao Jose do Rio Preto (FAMERP), Sao Jose do Rio Preto 15090-000, SP, Brazil., Tunissiolli NM; Research Unit of Genetics and Molecular Biology (UPGEM), Department of Molecular Biology, Faculty of Medicine of Sao Jose do Rio Preto (FAMERP), Sao Jose do Rio Preto 15090-000, SP, Brazil., Oliveira ARCP; Research Unit of Genetics and Molecular Biology (UPGEM), Department of Molecular Biology, Faculty of Medicine of Sao Jose do Rio Preto (FAMERP), Sao Jose do Rio Preto 15090-000, SP, Brazil., Mattos MF; Research Unit of Genetics and Molecular Biology (UPGEM), Department of Molecular Biology, Faculty of Medicine of Sao Jose do Rio Preto (FAMERP), Sao Jose do Rio Preto 15090-000, SP, Brazil., Galbiatti-Dias ALS; Research Unit of Genetics and Molecular Biology (UPGEM), Department of Molecular Biology, Faculty of Medicine of Sao Jose do Rio Preto (FAMERP), Sao Jose do Rio Preto 15090-000, SP, Brazil., Kawasaki-Oyama RS; Research Unit of Genetics and Molecular Biology (UPGEM), Department of Molecular Biology, Faculty of Medicine of Sao Jose do Rio Preto (FAMERP), Sao Jose do Rio Preto 15090-000, SP, Brazil., Pavarino EC; Research Unit of Genetics and Molecular Biology (UPGEM), Department of Molecular Biology, Faculty of Medicine of Sao Jose do Rio Preto (FAMERP), Sao Jose do Rio Preto 15090-000, SP, Brazil., da Silva RF; Department of Surgery and Liver Transplantation, Hospital de Base/FUNFARME/FAMERP-Foundation Faculty of Medicine of Sao Jose do Rio Preto, Sao Jose do Rio Preto 15090-000, SP, Brazil., Goloni-Bertollo EM; Research Unit of Genetics and Molecular Biology (UPGEM), Department of Molecular Biology, Faculty of Medicine of Sao Jose do Rio Preto (FAMERP), Sao Jose do Rio Preto 15090-000, SP, Brazil. |
| المصدر: | Genes [Genes (Basel)] 2022 Jan 30; Vol. 13 (2). Date of Electronic Publication: 2022 Jan 30. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: MDPI Country of Publication: Switzerland NLM ID: 101551097 Publication Model: Electronic Cited Medium: Internet ISSN: 2073-4425 (Electronic) Linking ISSN: 20734425 NLM ISO Abbreviation: Genes (Basel) Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Basel : MDPI |
| مواضيع طبية MeSH: | Carcinoma, Hepatocellular*/genetics , Carcinoma, Hepatocellular*/pathology , Liver Neoplasms*/genetics , Liver Neoplasms*/pathology , MicroRNAs*/genetics , MicroRNAs*/metabolism, Cell Line ; Gene Expression Regulation, Neoplastic ; Humans ; Vascular Endothelial Growth Factor A/genetics ; Vascular Endothelial Growth Factor A/metabolism |
| مستخلص: | MicroRNAs (miRNAs) are short non-coding RNA molecules acting as important posttranscriptional gene and protein expression regulators in cancer. The study goal was to examine VEGFA (vascular endothelial growth factor A) expression in hepatocellular carcinoma (HCC) cell lines upon transfection miR-612, miR-637, or miR-874. Methods: MiR-612 mimics, miR-637 mimics, or miR-874 inhibitors were transfected using Lipofectamine RNAiMax in both HCC cell lines, HepG2 and HuH-7. Real-time PCR, Western blotting, and ELISA methods were used to evaluate VEGFA regulation by the miRNAs. Results: Gene and protein expression levels of VEGFA were down-expressed in both cell lines, HepG2 and HuH-7, transfected with miR-612 or miR-637. Transfection with miR-874 inhibitor showed an increase in VEGFA gene expression in HepG2 and HuH-7 cell lines; however, no regulation was observed on VEGFA protein expression by miR-874 inhibition. Correlation analysis between miRNAs and VEGFA protein expression showed that miR-637 and miR-874 expression present inversely correlated to VEGFA protein expression. Conclusions : VEGFA was down-regulated in response to hsa-miR-612 or hsa-miR-637 overexpression; however, the modulation of VEGFA by miR-874 was observed only at the gene expression and thus, needs further investigation. |
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| فهرسة مساهمة: | Keywords: angiogenesis; cancer biomarkers; gene expression; liver neoplasms; microRNAs; transfection |
| المشرفين على المادة: | 0 (MIRN612 microRNA, human) 0 (MIRN637 microRNA, human) 0 (MIRN874 microRNA, human) 0 (MicroRNAs) 0 (VEGFA protein, human) 0 (Vascular Endothelial Growth Factor A) |
| تواريخ الأحداث: | Date Created: 20220225 Date Completed: 20220421 Latest Revision: 20220421 |
| رمز التحديث: | 20240628 |
| مُعرف محوري في PubMed: | PMC8871716 |
| DOI: | 10.3390/genes13020282 |
| PMID: | 35205327 |
| قاعدة البيانات: | MEDLINE |
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