دورية أكاديمية
أعرض في EDS

Activity dependent dissociation of the Homer1 interactome.

التفاصيل البيبلوغرافية
العنوان: Activity dependent dissociation of the Homer1 interactome.
المؤلفون: Stillman M; Center for Integrative Brain Research, Seattle Children's Research Institute, Seattle, WA, USA.; Dartmouth-Hitchcock Medical Center Psychiatry Residency Program, Dartmouth, NH, USA., Lautz JD; Center for Integrative Brain Research, Seattle Children's Research Institute, Seattle, WA, USA., Johnson RS; Department of Genome Sciences, University of Washington, Seattle, WA, USA., MacCoss MJ; Department of Genome Sciences, University of Washington, Seattle, WA, USA., Smith SEP; Center for Integrative Brain Research, Seattle Children's Research Institute, Seattle, WA, USA. seps@uw.edu.; Department of Pediatrics, University of Washington, Seattle, WA, USA. seps@uw.edu.; Graduate Program in Neuroscience, University of Washington, Seattle, WA, USA. seps@uw.edu.
المصدر: Scientific reports [Sci Rep] 2022 Feb 25; Vol. 12 (1), pp. 3207. Date of Electronic Publication: 2022 Feb 25.
نوع المنشور: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : Nature Publishing Group, copyright 2011-
مواضيع طبية MeSH: Neurons*/metabolism , Synapses*/metabolism, Animals ; Cerebral Cortex/metabolism ; Homer Scaffolding Proteins/genetics ; Homer Scaffolding Proteins/metabolism ; Mice ; Mice, Knockout ; Microfilament Proteins/metabolism ; Nerve Tissue Proteins/genetics ; Nerve Tissue Proteins/metabolism
مستخلص: Neurons encode information by rapidly modifying synaptic protein complexes, which changes the strength of specific synaptic connections. Homer1 is abundantly expressed at glutamatergic synapses, and is known to alter its binding to metabotropic glutamate receptor 5 (mGlu5) in response to synaptic activity. However, Homer participates in many additional known interactions whose activity-dependence is unclear. Here, we used co-immunoprecipitation and label-free quantitative mass spectrometry to characterize activity-dependent interactions in the cerebral cortex of wildtype and Homer1 knockout mice. We identified a small, high-confidence protein network consisting of mGlu5, Shank2 and 3, and Homer1-3, of which only mGlu5 and Shank3 were significantly reduced following neuronal depolarization. We identified several other proteins that reduced their co-association in an activity-dependent manner, likely mediated by Shank proteins. We conclude that Homer1 dissociates from mGlu5 and Shank3 following depolarization, but our data suggest that direct Homer1 interactions in the cortex may be more limited than expected.
(© 2022. The Author(s).)
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معلومات مُعتمدة: P41 GM103533 United States GM NIGMS NIH HHS; R01 MH113545 United States MH NIMH NIH HHS; R01 MH121487 United States MH NIMH NIH HHS
المشرفين على المادة: 0 (Homer Scaffolding Proteins)
0 (Homer1 protein, mouse)
0 (Microfilament Proteins)
0 (Nerve Tissue Proteins)
0 (Shank2 protein, mouse)
0 (Shank3 protein, mouse)
تواريخ الأحداث: Date Created: 20220226 Date Completed: 20220412 Latest Revision: 20221024
رمز التحديث: 20240829
مُعرف محوري في PubMed: PMC8881602
DOI: 10.1038/s41598-022-07179-3
PMID: 35217690
قاعدة البيانات: MEDLINE
الوصف
تدمد:2045-2322
DOI:10.1038/s41598-022-07179-3