دورية أكاديمية
أعرض في EDS

Reduction in Plasmodium falciparum Pfk13 and pfg377 allele diversity through time in southern Vietnam.

التفاصيل البيبلوغرافية
العنوان: Reduction in Plasmodium falciparum Pfk13 and pfg377 allele diversity through time in southern Vietnam.
المؤلفون: Thieu NQ; National Institute of Malariology, Parasitology and Entomology, Hanoi, Vietnam., Chinh VD; National Institute of Malariology, Parasitology and Entomology, Hanoi, Vietnam., Van Hanh T; National Institute of Malariology, Parasitology and Entomology, Hanoi, Vietnam., Van Dung N; National Institute of Malariology, Parasitology and Entomology, Hanoi, Vietnam., Takagi H; Department of Microbiology and Immunology, Aichi Medical University School of Medicine, Aichi, Japan., Annoura T; Department of Parasitology, National Institute of Infectious Diseases, Tokyo, Japan., Kawai S; Laboratory of Tropical Medicine and Parasitology, Dokkyo Medical University, Tochigi, Japan., Masuda G; Department of International Affairs and Tropical Medicine, Tokyo Women's Medical University, Tokyo, Japan.; School of Tropical Medicine and Global Health, Nagasaki University, 1-12-4 Sakamoto, Nagasaki, Nagasaki, 852-8523, Japan., Van Tuan N; Faculty of Medical Engineering, Phenikaa University, Hanoi, Vietnam., Hung VV; National Institute of Malariology, Parasitology and Entomology, Hanoi, Vietnam., Nakazawa S; Department of Protozoology, Institute of Tropical Medicine, Nagasaki University, Nagasaki, Japan., Culleton R; Department of Protozoology, Institute of Tropical Medicine, Nagasaki University, Nagasaki, Japan.; Division of Molecular Parasitology, Proteo-Science Centre, Ehime University, Ehime, Japan., Binh NTH; National Institute of Malariology, Parasitology and Entomology, Hanoi, Vietnam., Maeno Y; School of Tropical Medicine and Global Health, Nagasaki University, 1-12-4 Sakamoto, Nagasaki, Nagasaki, 852-8523, Japan. yoshimaeno@gmail.com.; Department of Virology and Parasitology, Fujita Health University School of Medicine, Aichi, Japan. yoshimaeno@gmail.com.
المصدر: Tropical medicine and health [Trop Med Health] 2022 Mar 01; Vol. 50 (1), pp. 19. Date of Electronic Publication: 2022 Mar 01.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: BioMed Central Ltd Country of Publication: Japan NLM ID: 101215093 Publication Model: Electronic Cited Medium: Print ISSN: 1348-8945 (Print) Linking ISSN: 13488945 NLM ISO Abbreviation: Trop Med Health Subsets: PubMed not MEDLINE
أسماء مطبوعة: Publication: <2016- > : London : BioMed Central Ltd.
Original Publication: Nagasaki, Japan : Japanese Society of Tropical Medicine, [2004]-
مستخلص: Background: Plasmodium falciparum has acquired resistance to artemisinin in Southeast Asia, with mutations in the P. falciparum Kelch-13 (Pfk13) gene associated with the resistance phenotype. The widespread use of Artemisinin-based combination therapy (ACT)s in Southeast Asia has led to the selection and spread of parasites carrying mutations in Pfk13. We characterised the allele diversity of Pfk13 and pfg377, an artemisinin-resistance neutral polymorphic gene, in parasite DNA extracted human blood from in southern Vietnam in 2003, 2012, 2015 and 2018.
Method: This study was conducted in Bu Gia Map commune, Binh Phuoc province, Vietnam, from May 2018 to January 2019. Twenty-four samples from 2018 to 2019, 30 from 2003, 24 from 2012 and 32 from 2015 were analysed. Malaria-infected human blood was collected by finger-prick and used for molecular analysis. A nested-PCR targeting the small subunit ribosomal RNA gene was used for Plasmodium species identification, followed by amplification and nucleotide sequencing of Pfk13 and region 3 of pfg377. Archived blood samples collected in the same region in 2012 and 2015 were also analysed as above for comparison.
Results: The genetic diversity of Pfk13 and pfg377 was lower in 2018-2019 compared to 2012 and 2015. The number of distinct Pfk13 mutants decreased from three in 2012 and 2015, P553L, V568G and C580Y, to one, C580Y in 2018-2019. In 2018-2019, the frequency of C580Y mutant strains was 71% (17/24 isolates). All samples were wild type in 2003. In 2012 and 2015, there were single-strain infections as well as co-infections with two mutant strains or with mutant and wild strains, whereas there were no co-infections in 2018. pfg377 allele diversity decreased from five alleles in 2012 to two alleles in 2018-2019.
Conclusion: The genetic diversity of P. falciparum was reduced at the two genetic loci surveyed in this study, Pfk13 and pfg377. In the case of the former gene, we observed an increase in the prevalence of parasites carrying the C580Y gene, known to confer reduced susceptibility to ACTs. The reduction in the diversity of pfg377 may be linked to the clonal expansion of parasite strains carrying the C580Y mutation, leading to an overall reduction in parasite genetic diversity across the population.
(© 2022. The Author(s).)
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معلومات مُعتمدة: 26360029 Japan Society for the Promotion of Science; 17H04513 Japan Society for the Promotion of Science; 20H04411 Japan Society for the Promotion of Science
فهرسة مساهمة: Keywords: Artemisinin resistance; Gametocyte; K13-propeller gene; Plasmodium falciparum; Vietnam; pfg377 gene
تواريخ الأحداث: Date Created: 20220302 Latest Revision: 20220318
رمز التحديث: 20240829
مُعرف محوري في PubMed: PMC8887123
DOI: 10.1186/s41182-022-00409-4
PMID: 35232492
قاعدة البيانات: MEDLINE
الوصف
تدمد:1348-8945
DOI:10.1186/s41182-022-00409-4