دورية أكاديمية
أعرض في EDS

A Glyphosate-Based Herbicide Cross-Selects for Antibiotic Resistance Genes in Bacterioplankton Communities.

التفاصيل البيبلوغرافية
العنوان: A Glyphosate-Based Herbicide Cross-Selects for Antibiotic Resistance Genes in Bacterioplankton Communities.
المؤلفون: Barbosa da Costa N; Département des Sciences Biologiques, Université de Montréal, Montreal, Canada.; Groupe de Recherche Interuniversitaire en Limnologie et Environnement Aquatique (GRIL), Montreal, Canada., Hébert MP; Groupe de Recherche Interuniversitaire en Limnologie et Environnement Aquatique (GRIL), Montreal, Canada.; Department of Biology, McGill Universitygrid.14709.3b, Montreal, Canada., Fugère V; Groupe de Recherche Interuniversitaire en Limnologie et Environnement Aquatique (GRIL), Montreal, Canada.; Québec Centre for Biodiversity Science (QCBS), Montreal, Canada.; Département des Sciences de l'Environnement, Université du Québec à Trois-Rivières, Trois-Rivières, Canada., Terrat Y; Département des Sciences Biologiques, Université de Montréal, Montreal, Canada., Fussmann GF; Groupe de Recherche Interuniversitaire en Limnologie et Environnement Aquatique (GRIL), Montreal, Canada.; Department of Biology, McGill Universitygrid.14709.3b, Montreal, Canada.; Québec Centre for Biodiversity Science (QCBS), Montreal, Canada., Gonzalez A; Department of Biology, McGill Universitygrid.14709.3b, Montreal, Canada.; Québec Centre for Biodiversity Science (QCBS), Montreal, Canada., Shapiro BJ; Département des Sciences Biologiques, Université de Montréal, Montreal, Canada.; Groupe de Recherche Interuniversitaire en Limnologie et Environnement Aquatique (GRIL), Montreal, Canada.; Québec Centre for Biodiversity Science (QCBS), Montreal, Canada.; Department of Microbiology and Immunology, McGill Universitygrid.14709.3b, Montreal, Canada.; McGill Genome Centre, McGill Universitygrid.14709.3b, Montreal, Canada.
المصدر: MSystems [mSystems] 2022 Apr 26; Vol. 7 (2), pp. e0148221. Date of Electronic Publication: 2022 Mar 10.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: American Society for Microbiology Country of Publication: United States NLM ID: 101680636 Publication Model: Print-Electronic Cited Medium: Print ISSN: 2379-5077 (Print) Linking ISSN: 23795077 NLM ISO Abbreviation: mSystems
أسماء مطبوعة: Original Publication: Washington, DC : American Society for Microbiology, [2016]-
مواضيع طبية MeSH: Herbicides*/pharmacology, Anti-Bacterial Agents/pharmacology ; Drug Resistance, Microbial ; Bacteria ; Aquatic Organisms ; Glyphosate
مستخلص: Agrochemicals often contaminate freshwater bodies, affecting microbial communities that underlie aquatic food webs. For example, the herbicide glyphosate has the potential to indirectly select for antibiotic-resistant bacteria. Such cross-selection could occur if the same genes (encoding efflux pumps, for example) confer resistance to both glyphosate and antibiotics. To test for cross-resistance in natural aquatic bacterial communities, we added a glyphosate-based herbicide (GBH) to 1,000-liter mesocosms filled with water from a pristine lake. Over 57 days, we tracked changes in bacterial communities with shotgun metagenomic sequencing and annotated metagenome-assembled genomes (MAGs) for the presence of known antibiotic resistance genes (ARGs), plasmids, and resistance mutations in the enzyme targeted by glyphosate (enolpyruvyl-shikimate-3-phosphate synthase; EPSPS). We found that high doses of GBH significantly increased ARG frequency and selected for multidrug efflux pumps in particular. The relative abundance of MAGs after a high dose of GBH was predictable based on the number of ARGs in their genomes (17% of variation explained) and, to a lesser extent, by resistance mutations in EPSPS. Together, these results indicate that GBHs can cross-select for antibiotic resistance in natural freshwater bacteria. IMPORTANCE Glyphosate-based herbicides (GBHs) such as Roundup formulations may have the unintended consequence of selecting for antibiotic resistance genes (ARGs), as demonstrated in previous experiments. However, the effects of GBHs on ARGs remain unknown in natural aquatic communities, which are often contaminated with pesticides from agricultural runoff. Moreover, the resistance provided by ARGs compared to canonical mutations in the glyphosate target enzyme, EPSPS, remains unclear. Here, we performed a freshwater mesocosm experiment showing that a GBH strongly selects for ARGs, particularly multidrug efflux pumps. These selective effects were evident after just a few days, and the ability of bacteria to survive and thrive after GBH stress was predictable by the number of ARGs in their genomes and, to a lesser extent, by mutations in EPSPS. Intensive GBH application may therefore have the unintended consequence of selecting for ARGs in natural freshwater communities.
References: Mol Biol Evol. 2021 May 19;38(6):2337-2350. (PMID: 33592098)
Bioinformatics. 2015 May 15;31(10):1674-6. (PMID: 25609793)
ISME J. 2020 Aug;14(8):2179-2196. (PMID: 32424247)
Nat Methods. 2014 Nov;11(11):1144-6. (PMID: 25218180)
Genome Res. 2016 Dec;26(12):1721-1729. (PMID: 27852649)
Front Public Health. 2018 Jan 22;5:361. (PMID: 29404314)
Nat Ecol Evol. 2020 Apr;4(4):578-588. (PMID: 32123321)
Nucleic Acids Res. 2010 Nov;38(20):e191. (PMID: 20805240)
Genome Res. 2015 Jul;25(7):1043-55. (PMID: 25977477)
Chemosphere. 2003 Aug;52(7):1189-97. (PMID: 12821000)
PLoS Pathog. 2011 Jul;7(7):e1002158. (PMID: 21811410)
PeerJ. 2015 Oct 08;3:e1319. (PMID: 26500826)
PLoS Comput Biol. 2020 Apr 3;16(4):e1007781. (PMID: 32243433)
FEBS J. 2011 Aug;278(16):2753-66. (PMID: 21668647)
Ecol Appl. 2021 Oct;31(7):e02423. (PMID: 34288209)
J Ind Microbiol Biotechnol. 2012 Apr;39(4):641-7. (PMID: 22089966)
Nucleic Acids Res. 2014 Jan;42(Database issue):D206-14. (PMID: 24293654)
Annu Rev Biochem. 2004;73:241-68. (PMID: 15189142)
Sci Total Environ. 2019 Mar 1;654:177-189. (PMID: 30445319)
Oecologia. 2001 Oct;129(2):271-280. (PMID: 28547606)
Mol Ecol. 2021 Oct;30(19):4771-4788. (PMID: 34324752)
Bioengineered. 2021 Dec;12(1):63-69. (PMID: 33345705)
Nucleic Acids Res. 2020 Jan 8;48(D1):D517-D525. (PMID: 31665441)
Bioinformatics. 2014 Aug 1;30(15):2114-20. (PMID: 24695404)
FEMS Microbiol Rev. 2011 Sep;35(5):957-76. (PMID: 21711367)
Int J Antimicrob Agents. 2019 Jun;53(6):767-773. (PMID: 30885807)
Sci Total Environ. 2018 Mar;616-617:255-268. (PMID: 29117584)
Environ Sci Eur. 2016;28(1):3. (PMID: 27752438)
Nucleic Acids Res. 2002 Jun 1;30(11):2478-83. (PMID: 12034836)
PLoS Comput Biol. 2011 Oct;7(10):e1002195. (PMID: 22039361)
PeerJ. 2018 Oct 12;6:e5801. (PMID: 30345180)
Nat Methods. 2012 Mar 04;9(4):357-9. (PMID: 22388286)
Environ Pollut. 2020 May;260:114012. (PMID: 31995771)
Microbiology (Reading). 2017 Dec;163(12):1791-1801. (PMID: 29139345)
Nat Microbiol. 2018 Aug;3(8):963. (PMID: 30042441)
BMC Bioinformatics. 2010 Mar 08;11:119. (PMID: 20211023)
Environ Sci Pollut Res Int. 2021 Nov;28(43):60635-60648. (PMID: 34160765)
mBio. 2015 Mar 24;6(2):. (PMID: 25805724)
Bioinformatics. 2009 Aug 15;25(16):2078-9. (PMID: 19505943)
Proc Natl Acad Sci U S A. 2018 Oct 9;115(41):10305-10310. (PMID: 30249635)
J Hazard Mater. 2021 Apr 15;408:124556. (PMID: 33243645)
Environ Sci Technol. 2020 Jul 21;54(14):8770-8778. (PMID: 32551597)
Pest Manag Sci. 2008 Apr;64(4):319-25. (PMID: 18273882)
J Mol Biol. 1990 Oct 5;215(3):403-10. (PMID: 2231712)
Protoplasma. 2016 May;253(3):691-707. (PMID: 26660352)
Trends Microbiol. 2006 Apr;14(4):176-82. (PMID: 16537105)
فهرسة مساهمة: Keywords: antibiotic efflux pump; antibiotic resistance genes; antimicrobial resistance; cross-selection; efflux pumps; glyphosate; herbicide; herbicides; indirect selection; mesocosm; metagenomics; natural selection
المشرفين على المادة: 0 (Herbicides)
0 (Anti-Bacterial Agents)
0 (shikimate)
تواريخ الأحداث: Date Created: 20220310 Date Completed: 20221221 Latest Revision: 20231213
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC9040730
DOI: 10.1128/msystems.01482-21
PMID: 35266795
قاعدة البيانات: MEDLINE
الوصف
تدمد:2379-5077
DOI:10.1128/msystems.01482-21