دورية أكاديمية
Metagenomic analysis of the intestinal microbiome in goats on cactus and Salicornia -based diets.
| العنوان: | Metagenomic analysis of the intestinal microbiome in goats on cactus and Salicornia -based diets. |
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| المؤلفون: | Domínguez FF; Public Technological Higher Education Institute 'July 24, Zarumilla,' Tumbes, Perú.; Agrarian Sciences Faculty, National University of San Martin, Tarapoto, Perú., Crisanto MEV; Health Sciences Faculty, National University of Tumbes, Tumbes, Perú., Castro RLS; Health Sciences Faculty, National University of Tumbes, Tumbes, Perú., Rojas LV; Public Technological Higher Education Institute 'July 24, Zarumilla,' Tumbes, Perú., Cuba VMB; Public Technological Higher Education Institute 'July 24, Zarumilla,' Tumbes, Perú., Santos GRS; Public Technological Higher Education Institute 'July 24, Zarumilla,' Tumbes, Perú., Ramos CAL; Public Technological Higher Education Institute 'July 24, Zarumilla,' Tumbes, Perú., Mialhe E; Molecular Biotechnology Laboratory, INCABIOTEC, Tumbes, Perú. |
| المصدر: | Open veterinary journal [Open Vet J] 2022 Jan-Feb; Vol. 12 (1), pp. 61-68. Date of Electronic Publication: 2022 Jan 23. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Faculty of Veterinary Medicine, University of Tripoli Country of Publication: Libya NLM ID: 101653182 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2218-6050 (Electronic) Linking ISSN: 22186050 NLM ISO Abbreviation: Open Vet J Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Nov. 2016 -: Tripoli, Libya : Faculty of Veterinary Medicine, University of Tripoli Original Publication: Tripoli, Libya : Faculty of Veterinary Medicine, University of Tripoli : National Authority for Scientific Research |
| مواضيع طبية MeSH: | Cactaceae*/genetics , Chenopodiaceae*/genetics , Gastrointestinal Microbiome*/genetics, Animals ; Bacteroidetes/genetics ; Diet/veterinary ; Goats ; RNA, Ribosomal, 16S/genetics ; Soil |
| مستخلص: | Background: The Peruvian coast is characterized by its arid and saline soils, the cactus being an alternative for arid soils and Salicornia for saline soils. Therefore, it is necessary to develop nutrition based on the intestinal microbiota in goats. Aim: To identify the intestinal microbiota in goats through a metagenomic analysis. Methods: In this study, goats and kids were randomly selected and fed cacti and Salicornia as potential forage species compared to native grass to study the changes in the microbiota using massive sequencing using the 16S rRNA gene as a marker. Results: The sequencing results showed the taxonomic levels of Bacteroidetes and Firmicutes at the phylum level as the most abundant in creole goats' microbiome, varying from 18% to 36% and 47% to 66%, respectively. At the genus level, variants of the genus Ruminococcaceae stand out, related to cellulose degradation, as the most dominant in all samples, followed by Christensenellaceae , Rikenellaceae , and Prevotellaceae . Also, the genus Akkermansia appeared in greater abundance in kids fed with cactus, being necessary for being related to the intestinal mucosa's health and avoiding the adhesion of pathogens to the intestinal epithelium. Conclusion: These microbiota changes based on diets with high fiber content are necessary to understand the adaptation of this species to favorable dietary changes. Competing Interests: The authors declare that there is no conflict of interest. |
| References: | Sci Rep. 2015 Nov 13;5:16643. (PMID: 26563823) Can J Microbiol. 2019 Dec;65(12):859-869. (PMID: 31386822) Microbiome. 2015 Jul 01;3:28. (PMID: 26167280) BMC Microbiol. 2017 Sep 13;17(1):194. (PMID: 28903732) Clin Microbiol Rev. 2015 Jan;28(1):237-64. (PMID: 25567229) Nat Methods. 2013 Jan;10(1):57-9. (PMID: 23202435) Proc Natl Acad Sci U S A. 2013 Feb 26;110(9):3229-36. (PMID: 23391737) Front Microbiol. 2019 Feb 20;10:239. (PMID: 30873128) PLoS Biol. 2013;11(8):e1001631. (PMID: 23976878) PLoS One. 2020 Jan 30;15(1):e0228496. (PMID: 31999767) Proc Natl Acad Sci U S A. 2011 Mar 15;108 Suppl 1:4516-22. (PMID: 20534432) World J Microbiol Biotechnol. 2014 Feb;30(2):669-80. (PMID: 24068532) ISME J. 2012 Aug;6(8):1449-58. (PMID: 22437156) Proc Natl Acad Sci U S A. 2010 Nov 2;107(44):18933-8. (PMID: 20937875) Pediatr Res. 2012 Jul;72(1):77-85. (PMID: 22453296) Front Microbiol. 2017 Aug 24;8:1605. (PMID: 28883813) Microb Pathog. 2017 May;106:171-181. (PMID: 26875998) Microb Ecol. 2010 Apr;59(3):511-22. (PMID: 20037795) PLoS Biol. 2018 May 7;16(5):e2005653. (PMID: 29734329) J Appl Microbiol. 2016 Mar;120(3):560-70. (PMID: 26600313) BMC Genomics. 2017 Oct 18;18(1):800. (PMID: 29047329) Bioinformatics. 2011 Aug 15;27(16):2194-200. (PMID: 21700674) FEMS Microbiol Ecol. 2011 Nov;78(2):275-84. (PMID: 21692816) |
| فهرسة مساهمة: | Keywords: Bacteria; Bioinformatic; Forage; Microbiota; Ruminants |
| المشرفين على المادة: | 0 (RNA, Ribosomal, 16S) 0 (Soil) |
| تواريخ الأحداث: | Date Created: 20220328 Date Completed: 20220418 Latest Revision: 20220418 |
| رمز التحديث: | 20231215 |
| مُعرف محوري في PubMed: | PMC8956232 |
| DOI: | 10.5455/OVJ.2022.v12.i1.7 |
| PMID: | 35342733 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 2218-6050 |
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| DOI: | 10.5455/OVJ.2022.v12.i1.7 |