دورية أكاديمية
In Vivo Visualization of Mobile mRNA Particles in Plants Using BglG.
| العنوان: | In Vivo Visualization of Mobile mRNA Particles in Plants Using BglG. |
|---|---|
| المؤلفون: | Peña EJ; Instituto de Biotecnología y Biología Molecular (IBBM), Facultad de Ciencias Exactas-UNLP, CONICET, La Plata, Argentina., Heinlein M; Institut de Biologie Moléculaire des Plantes (IBMP), CNRS, Université de Strasbourg, Strasbourg, France. manfred.heinlein@ibmp-cnrs.unistra.fr. |
| المصدر: | Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2022; Vol. 2457, pp. 411-426. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Totowa, NJ : Humana Press Original Publication: Clifton, N.J. : Humana Press, |
| مواضيع طبية MeSH: | Escherichia coli*/genetics , Plant Viral Movement Proteins*/metabolism, RNA, Messenger/genetics ; RNA, Messenger/metabolism ; RNA, Viral/genetics ; RNA, Viral/metabolism ; Nicotiana/metabolism |
| مستخلص: | Cells have developed mechanisms for cytoplasmic RNA transport and localization that participate in the regulation and subcellular localization of protein synthesis. In addition, plants can exchange RNA molecules between cells through plasmodesmata and to distant tissues in the phloem. These mechanisms are hijacked by RNA viruses to establish their replication complexes and to disseminate their genomes throughout the plant organism with the help of virus-encoded movement proteins (MP). Live imaging of RNA molecules is a fundamental approach to understand the regulation and molecular basis of these processes. The most widely used experimental systems for the in vivo visualization of genetically encoded RNA molecules are based on fluorescently tagged RNA binding proteins that bind to specific motifs inserted into the RNA, thus allowing the tracking of the specific RNA molecule by fluorescent microscopy. Recently, we developed the use of the E. coli RNA binding protein BglG for the imaging of RNAs tagged with BglG-binding sites in planta. We describe here the detailed method by which we use this in vivo RNA tagging system for the real-time imaging of Tobacco mosaic virus (TMV) MP mRNA. (© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.) |
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| فهرسة مساهمة: | Keywords: BglG; Movement protein (MP); RNA imaging; RNA labeling; RNA localization; RNA transport; RNA visualization; Tobacco mosaic virus (TMV) |
| المشرفين على المادة: | 0 (Plant Viral Movement Proteins) 0 (RNA, Messenger) 0 (RNA, Viral) |
| تواريخ الأحداث: | Date Created: 20220329 Date Completed: 20220401 Latest Revision: 20231213 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1007/978-1-0716-2132-5_28 |
| PMID: | 35349157 |
| قاعدة البيانات: | MEDLINE |
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